[1]赵颖雷,朱祝军,任莉.“东方蜜一号”甜瓜组织培养与快速繁殖研究[J].北方园艺,2012,36(18):122-124.
　ZHAO Ying-lei,ZHU Zhu-jun,REN Li.Study on Tissue Culture and Rapid Propagation of ‘Dongfangmi No.1’ Melon[J].Northern Horticulture,2012,36(18):122-124.
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“东方蜜一号”甜瓜组织培养与快速繁殖研究

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第36卷 期数: 2012年18期 页码: 122-124 栏目: 出版日期: 2012-09-30
Title:
Study on Tissue Culture and Rapid Propagation of ‘Dongfangmi No.1’ Melon
文章编号:
1001-0009(2012)18-0122-03
作者:
赵颖雷1; 朱祝军2; 任莉3
1.浙江农林大学 风景园林与建筑学院，浙江 临安 311300；
2.浙江农林大学 农业与食品科学学院，浙江 临安 311300；
3.新疆石河子大学 农学院，新疆 石河子 832000
Author(s):
ZHAO Ying-lei1; ZHU Zhu-jun2; REN Li3
1.College of Landscape and Architecture，Zhejiang Agricultural and Forestry University，Lin’an，Zhejiang 311300；
2.College of Agriculture and Food Science，Zhejiang Agricultural and Forestry University，Lin’an，Zhejiang 311300;
3.College of Agronomy，Shehezi University，Shehezi，Xinjiang 832000
关键词:
“东方蜜一号”甜瓜; 组织培养; 快速繁殖
Keywords:
Dongfangmi No.1’ melon; tissue culture; rapid propagation
分类号:
S 652.603.6
文献标志码:
B
摘要:
选取“东方蜜一号”甜瓜的新生叶片为外植体，接种于含有不同激素配比的MS培养基中，诱导愈伤组织、不定芽与不定根的形成，比较不同激素浓度对外植体脱分化和再分化的影响。结果表明：“东方蜜一号”甜瓜叶片离体培养的最佳愈伤组织诱导培养基为MS+2,4-D0.10 mg/L+6-BA 1.50 mg/L，诱导率达91%，最佳不定芽诱导培养基为MS+2,4-D 0.01 mg/L+6-BA 1.50 mg/L，平均不定芽个数为3.52个；最适生根诱导培养基为MS+2,4-D 0.05 mg/L+NAA 0.20 mg/L+IBA 0.60 mg/L,生根率达85%。
Abstract:
New borning leaves of ‘Dongfangmi No.1’ melon were selected as explant and planted into the MS medium with different concentration and variety of endocrine to induce the formation of callus and adventitious buds and roots.The results showed that the most ideal medium for callus inducing was MS+2,4-D 0.10 mg/L+6-BA 1.50 mg/L with the best induction rate 91%；The most ideal medium for adventitious buds inducing was MS+2,4-D 0.01 mg/L+6-BA 1.50 mg/L with the best average 3.52；the best rooting medium was MS+2,4-D 0.05 mg/L+NAA 0.20 mg/L+IBA 0.60 mg/L with the best rooting rate 85%.

参考文献/References:

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备注/Memo

第一作者简介：赵颖雷（1986-），男，在读硕士，研究方向为园林植物与观赏园艺。

责任作者：朱祝军（1968-），男，博士，教授，博士生导师，现主要从事园艺作物生理和分子生物学研究工作。E-mail：Zhuzj@zafu.edu.cn.
基金项目：浙江省大学生科技创新活动计划资助项目。

更新日期/Last Update: 2014-08-29