SUN Rui,SHAO Hong,LIU Juan,et al.Study on POD Isoenzymes of Aristolochia contorta Bunge at Vegetative Growth Phase[J].Northern Horticulture,2012,36(16):86-87.
不同红掌品种在组培生产上的差异表现
- Title:
- Study on POD Isoenzymes of Aristolochia contorta Bunge at Vegetative Growth Phase
- 文章编号:
- 1001-0009(2012)16-0086-02
- Keywords:
- Aristolochia contorta Bunge; vegetative growth phase; POD; isoenzyme
- 分类号:
- S 682.1+4
- 文献标志码:
- B
- 摘要:
- 以“亚利桑那”、“阿拉巴马”、“粉冠军”3个红掌品种根茎、叶柄、叶片为外植体,研究比较了3个品种的外植体诱导、继代增殖、生根培养的差异表现。结果表明：根茎诱导无菌芽成功率为86.9~90.0%,叶片诱导愈伤组织成功率为80%~84%,叶柄诱导愈伤组织成功率为64%~68%,根茎一般较难诱导出愈伤组织；不同品种的红掌增殖继代的增殖倍率不同，“粉冠军”最高为6.8,其次为“亚利桑那”5.8,“阿拉巴马”为3.8。红掌的生根较易,不同品种的红掌生根率均可达到95%以上。
- Abstract:
- The POD enzymes from root，tender stem，old stem，petiole，unexpanded tender leaf，expanded tender leaf and mature leaf of Aristolochia contorta Bunge at vegetative growth phase were analyzed by vertical polyacrylamide gel electrophoresis (PAGE).The results showed that 10 bands of POD isoenzymes from 7 different tissues and organs of Aristolochia contorta Bunge at vegetative growth phase were expressed and POD isoenzymes had a tissue specificity.Unexpanded tender leaf which had abundant enzyme bands，stronger activity and higher content were the good materials for analysis on POD isoenzyme.There were the fewest enzyme bands in mature leaf，so it was not fit for analysis on POD isoenzyme.
参考文献/References:
[1]冯毓秀,林寿全,张秀琴.国产马兜铃属的植物和生药研究:资源利用[J].药学学报,1983,18(4):291-298. [2]中国科学院植物研究所.中国高等植物图鉴(第一册)[M].北京:科学出版社,1972:548. [3]李俊.国产马兜铃科（Aristolochiaceae）植物比较形态学研究[D].西安:陕西师范大学,2008. [4]程汝凤.青木香及其混淆品的鉴别[J].基层中药杂志,1996,10(3):12. [5]王智民.现代中药的化学研究方法[J].中国中药杂志,2000,25(2):70. [6]张海平,房伟民,陈发棣,等.睡莲不同器官和不同生长阶段的过氧化物酶和酯酶同工酶比较[J].浙江林学院学报,2010,27(2):246-250. [7]徐秀芳,张海洋,张丽敏.不同榛树叶中过氧化物酶同工酶的研究[J].林业科技,2005,30(2):1-3. [8]刘金龙,孙东发.“恩五叶蜜”绞股蓝过氧化氢同工酶研究[J].时珍国医国药,2006,17(10):7891-7891. [9]邵红,李秀霞,李修平,等.杂交榛抗寒变异愈伤组织的选择[J].林业科技,2011,36(4):8-10. [10]徐金星,刘忠民,刘翠明.大豆品种接种SMV叶片POD同工酶的研究[J].东北农业大学学报,2000,31(1):20-25. [11]丁玲,陈发棣,滕年军,等.菊花不同生长阶段不同器官POD和EST同工酶比较[J].西北植物学报,2007,27(10)：2029-2034.
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备注/Memo
第一作者简介：吴红英（1975-），女，本科，高级工程师，现从事林木花卉繁育研究及推广工作。E-mail:wuhongying2000-1@sohu.com.