CUI Wei,ZHANG Mei-juan,SHA Wei.Screen of Sterilized Methods and Inoculation Culture Medium Filter for Explants of Grimmia pilifera[J].Northern Horticulture,2012,36(10):138-140.
毛尖紫萼藓外植体消毒方法及接种培养基的筛选
- Title:
- Screen of Sterilized Methods and Inoculation Culture Medium Filter for Explants of Grimmia pilifera
- 文章编号:
- 1001-0009（2012）10-0138-03
- Keywords:
- Grimmia pilifera; tissue culture; sterilization method; culture medium
- 分类号:
- S 603.6
- 文献标志码:
- A
- 摘要:
- 以毛尖紫萼藓(Grimmia pilifera P.Beauv.)配子体为试材，以Benek、MS、Knop 3种培养基为基本培养基,通过预培养和预处理，借助旋流振荡器，对毛尖紫萼藓组织培养消毒方法及接种培养基类型进行了筛选。结果表明：预培养15 d左右的外植体,0.025%升汞溶液旋流振荡105 s能有效地对外植体进行消毒,Benek培养基适合毛尖紫萼藓返绿生长。
- Abstract:
- Chosen Grimmia pilifera gametophytic as test material,Benek，MS，Knop as basic culture medium,the tissue culture of disinfection method and inoculation medium types were screened,through pre-culture,pretreatment,had the aid of swirl oscillator.The results indicated that explants pre-culture for about fifteen days，swirl oscillating by 0.025% mercuric chloride solution for 105 seconds cound disinfect effectively for explants.Benek culture medium was suitable for Grimmia pilifera to return to green growth.
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备注/Memo
第一作者简介：崔巍（1985-），男，硕士,研究方向为苔藓植物组织培养。E-mail:cuiwei313@163.com。责任作者：沙伟（1963-），女，博士，教授，博士生导师，现从事植物学和植物遗传学教学与科研工作。E-mail: shw1129@263.net。
基金项目：国家自然科学基金资助项目（31070180）。
收稿日期：2012-02-24