GUAN Qiuzhu,DONG Xiaochang,DONG Ran,et al.Study on Tissue Culture Rapid Propagation and Tissue Micro-cuttage Technology of Pyrus betulifolia[J].Northern Horticulture,2024,(20):23-29.[doi:10.11937/bfyy.20241744]
杜梨组培快繁及组培苗微扦插技术研究
- Title:
- Study on Tissue Culture Rapid Propagation and Tissue Micro-cuttage Technology of Pyrus betulifolia
- 文章编号:
- 1001-0009(2024)20-0023-07
- 分类号:
- S 661.2
- 文献标志码:
- A
- 摘要:
- 以种子来源的4个杜梨株系组培苗为试材，采用植物组织培养和组培苗微扦插的方法，研究了不同浓度细胞分裂素6-BA和IBA处理对杜梨丛生芽增殖的影响以及不同浓度IBA和生长素（NAA）处理对杜梨组培苗离体生根和微扦插生根的影响，以期为稳定高效杜梨组培快繁体系的建立提供参考依据。结果表明：基因型和6-BA浓度对杜梨增殖系数具有极显著影响，IBA浓度对增殖系数具有显著影响；低浓度的生长素更适合用于杜梨组培苗的生根诱导。综合考虑组培苗增殖和生根情况，DL30株系表现最优，其适宜的增殖培养基为MS＋1.0 mg·L-1 6-BA＋0.2 mg·L-1 IBA，增殖系数为3.4±0.35，适宜的生根培养基为 1/2MS+0.2 mg·L-1 IBA，生根率达60.0%±20.0%。IBA和NAA 蘸根处理对杜梨组培苗微扦插生根有显著影响，杜梨株系DL30组培苗通过1 000 mg·L-1的IBA蘸根处理后微扦插生根率为46.7%±8.8%。
- Abstract:
- Tissue culture plantlets of 4 Pyrus betulifolia lines originated from seed were used as the test materials.The methods of plant tissue culture and tissue culture seedling micro-cutting were adopted.The effects of different concentrations of 6-BA singly or in combination with IBA on the in vitro shoot proliferation and the influence of different concentrations of IBA or NAA on rooting of micro-shoots by in vitro and ex vitro was studied,in order to provide reference for the establishment of the tissue culture and rapid proliferation system of Pyrus betulifolia.The results showed that the genotype and 6-BA concentration had a highly significant effect on the proliferation coefficient，and the IBA concentration had a significant effect on the proliferation coefficient.As for root induction，low concentration of auxin was better than high concentration.DL30 showed higher proliferation coefficient and rooting rate.The optimum medium for DL30 shoot proliferation was MS＋1.0 mg·L-1 6-BA＋0.2 mg·L-1 IBA，with a proliferation coefficient 3.4±0.35.For the in vitro rooting experiments，the highest rooting rate of DL30 was 60.0%±20.0% on the medium containing 1/2MS+0.2 mg·L-1 IBA.For the ex vitro rooting experiments，rooting of micro-shoots was significantly influenced by auxin applied as quick-dip method，and the highest rooting rate (46.7%±8.8%) was obtained in DL30 at the treatment of 1 000 mg·L-1 IBA.
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备注/Memo
第一作者简介：关秋竹（1981-），女，博士，助理研究员，现主要从事果树生物技术等研究工作。E-mail：guanqiuzhu@163.com.责任作者：魏树伟（1981-），男，博士，副研究员，现主要从事梨育种及栽培等研究工作。E-mail：weisw2007@163.com.基金项目：国家梨产业技术体系资助项目（CARS-28-37）；中央引导地方科技发展专项资助项目（YDZX2023067）；国家盐碱地综合利用技术创新中心“揭榜挂帅”资助项目（GYJ202300X）。收稿日期：2024-04-23