ZHU Chunyao,SUN Xiaogang,SUN Hongtong,et al.Establishment and Optimization of Tissue Culture System of Four Hosta plantagina Varieties[J].Northern Horticulture,2024,(18):49-58.[doi:10.11937/bfyy.20240563]
四个玉簪品种组织培养体系的建立与优化
- Title:
- Establishment and Optimization of Tissue Culture System of Four Hosta plantagina Varieties
- 文章编号:
- 1001-0009(2024)18-0049-10
- Keywords:
- Hosta plantagina; tissue culture; plant growth regulators; proliferation and differentiation; seedling transplanting
- 分类号:
- S 682
- 文献标志码:
- A
- 摘要:
- 以4个玉簪品种的幼芽外植体为试材，采用植物组织培养技术，研究了外植体不同灭菌时间、植物激素配比及浓度对不定芽诱导、增殖和生根以及不同基质配方对炼苗移栽的影响，以期为丰富东北地区的玉簪资源、丰富市场需求及玉簪工厂化生产提供参考依据。结果表明：最佳的外植体灭菌方式为75%酒精30 s+0.1%氯化汞溶液8 min；最佳丛生芽诱导培养基配方分别采用‘小黄叶’为MS+6-BA 3.5 mg·L-1+NAA 0.1 mg·L-1、‘金头饰’为MS+6-BA 2.5 mg·L-1+NAA 0.2 mg·L-1、‘莲花’和‘蓝男孩’为MS+6-BA 2.5 mg·L-1+NAA 0.1 mg·L-1；‘莲花’与‘蓝男孩’在MS+6-BA 3.0 mg·L-1+NAA 0.1 mg·L-1的继代增殖培养基下表达出高的增殖倍数,分别为4.06、6.71倍；‘金头饰’在MS+6-BA 4.0 mg·L-1+NAA 0.1 mg·L-1的诱导下,增殖倍数可达4.23；‘小黄叶’则在MS+6-BA 2.0 mg·L-1+0.2 mg·L-1 NAA的诱导下可增殖4.25倍；最佳生根培养基为‘小黄叶’1/2MS+IBA 0.5 mg·L-1、‘金头饰’和‘蓝男孩’1/2MS+NAA 0.05 mg·L-1、‘莲花’1/2MS+NAA 0.1 mg·L-1。炼苗试验中，最适宜做‘莲花’和‘蓝男孩’玉簪组培苗移栽的基质是上砂下土（厚度1∶1）的基质，成活率达100%；而‘小黄叶’和‘金头饰’玉簪选用砂∶土(1∶1)混合基质移栽的效果最好。
- Abstract:
- Taking the young shoot explants of four Hosta plantagina varieties as the test materials,the effects of different sterilisation times,phytohormone ratios and concentrations on the induction,proliferation and rooting of adventitious shoots,as well as the effects of different substrate formulations on seedling transplantation were investigated,in order to provide reference for enriching the resources of Hosta plantagina in the Northeast China,and for enriching the market demand as well as the factory production of Hosta plantagina.The results showed that the optimal sterilization method for explants was 75% alcohol 30 seconds+0.1% mercuric chloride solution for 8 minutes.The optimal formulations of clumping bud induction medium were H.‘Indigo Leaf’ MS+6-BA 3.5 mg·L-1+NAA 0.1 mg·L-1,H.‘Golden Tiara’ MS+6-BA 2.5 mg·L-1+NAA 0.2 mg·L-1,H.‘Flos Lotus’ and H.‘Blue Cadet’ MS+6-BA 2.5 mg·L-1+NAA 0.1 mg·L-1; H.‘Flos Lotus’and H.‘Blue Cadet’ showed high value-added of 4.06 and 6.71 times,respectively,under the induction of MS+6-BA 3.0 mg·L-1+NAA 0.1 mg·L-1,‘Golden Tiara’ showed 4.23 times under the induction of MS+6-BA 4.0 mg·L-1+NAA 0.1 mg·L-1.H.‘Indigo Leaf’ could proliferate 4.25-fold under the induction of MS+6-BA 2.0 mg·L-1+NAA 0.2 mg·L-1,and the optimal rooting medium was H.‘Indigo Leaf’ 1/2MS+IBA 0.5 mg·L-1,H.‘Golden Tiara’ and H.‘Blue Cadet’ 1/2MS+NAA 0.05 mg·L-1,and H.‘Flos Lotus’,1/2MS+NAA 0.1 mg·L-1.In the seedling refining test,the most suitable substrate for transplanting H.‘Flos Lotus’ and H.‘Blue Cadet’ tissue culture seedlings was the substrate of upper sand and lower soil (thickness 1∶1),and the survival rate was 100%.The H.‘Indigo Leaf’ and H.‘Golden Tiara’ were transplanted with sand∶soil (1∶1) mixed substrate for transplanting.
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备注/Memo
第一作者简介：朱纯瑶（2000-），女，硕士研究生，研究方向为园林植物与生态修复。E-mail：1493770448@qq.com.责任作者：孙晓刚（1969-），男，硕士，教授，现主要从事园林植物应用与风景园林规划设计等研究工作。E-mail：sunxiaogang@jlau.edu.cn.基金项目：吉林省科技发展计划资助项目（20230202100NC）。收稿日期：2024-02-02