LI Xiuqi,JIA Baohua,HAO Haohao,et al.Investigation and Pathogen Identification of Watermelon Virus Disease in Typical Hilly Area of South Taihang[J].Northern Horticulture,2024,(11):35-39.[doi:10.11937/bfyy.20233447]
南太行典型丘陵山区西瓜病毒病调查及病原鉴定
- Title:
- Investigation and Pathogen Identification of Watermelon Virus Disease in Typical Hilly Area of South Taihang
- 文章编号:
- 1001-0009(2024)11-0035-05
- Keywords:
- watermelon; viral disease; pathogen identification; South Taihang
- 分类号:
- S 651
- 文献标志码:
- A
- 摘要:
- 以南太行典型丘陵山区露地和小拱棚种植西瓜为试材，对其病毒病的田间发生情况及发病症状进行了调查，采用小RNA 测序和RT-PCR方法研究了侵染西瓜病毒病的病原，以期为该地区西瓜病毒病防控策略制定提供参考依据。结果表明：该地区西瓜病毒病田间一般从5月上中旬开始发病，症状以花叶、皱缩为主，使用16种病毒引物在14个病样中共检测出7种病毒，分别是西瓜花叶病毒（WMV）、小西葫芦黄花叶病毒（ZYMV）、番木瓜畸形花叶病毒（PLDMV）、甜瓜蚜传黄化病毒（MABYV）、西瓜皱叶相关病毒 2号（WCLaV2）、西瓜潜隐病毒（CiLCV）和甜瓜混合病毒 1号（CMAV1）。其中，WMV、CiLCV和CMAV1 3种病毒检出率为100.0%，MABYV病毒检出率最低，为28.6%。田间西瓜病毒病发生均为复合侵染，试验中病样一般感染4～7种不同种类的病毒。进一步对WMV的全长基因组核苷酸序列进行一致性比较和分析，发现来自该区域的WMV分离物具有较高的遗传多样性，表明该病毒在该地区可能存在多个初始侵染源。
- Abstract:
- The field occurrence and symptoms of watermelon virus disease were used as test materials and were investigated in the open field and small arch shed planted in the typical hilly area of South Taihang，and the pathogen of watermelon virus disease was studied by small RNA sequencing and RT-PCR，in order to provide reference for the development of watermelon virus disease prevention and control strategy in this area.The results showed that the watermelon virus disease in the field generally started from early to middle of May，and the symptoms were mainly mosaic and wrinkling.A total of 7 viruses were detected in 14 disease samples using 16 virus primers.They were watermelon mosaic virus (WMV)，zucchini yellow mosaic virus (ZYMV)，papaya malformed mosaic virus (PLDMV)，melon aphid yeltization virus (MABYV)，watermelon ruffle associated virus 2 (WCLaV2)，watermelon crypto-virus (CiLCV) and melon mixed virus 1 (CMAV1).The detection rate of WMV，CiLCV and CMAV1 virus was 100%，and the detection rate of MABYV virus was the lowest (28.6%).In the field，watermelon virus disease occurred in complex infection，and the disease samples were generally infected with 4-7 different kinds of viruses.Further consistency comparison and analysis of the full-length genomic nucleotide sequence of WMV showed that WMV isolates from this region had high genetic diversity，indicating that the virus may have multiple initial infection sources in this region.
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备注/Memo
第一作者简介：李秀启（1975-），男，硕士，副研究员，现主要从事高效农业及农业产业发展等研究工作。E-mail：13938176372@139.com.基金项目：济源科技攻关资助项目（23021009）。收稿日期：2023-10-11