[1]孙波,易宝元,杨连花,等.湖南主栽西瓜品种指纹图谱构建及遗传多样性分析[J].北方园艺,2023,(10):31-36.[doi:10.11937/bfyy.20223483]
　SUN Bo,YI Baoyuan,YANG Lianhua,et al.Establishment of Fingerprint and Genetic Diversity Analysis of Main Watermelon Cultivars in Hunan Province[J].Northern Horticulture,2023,(10):31-36.[doi:10.11937/bfyy.20223483]
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湖南主栽西瓜品种指纹图谱构建及遗传多样性分析

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 期数: 2023年10 页码: 31-36 栏目: 出版日期: 2023-05-30
Title:
Establishment of Fingerprint and Genetic Diversity Analysis of Main Watermelon Cultivars in Hunan Province
作者:
孙波1; 2; 易宝元1; 杨连花1; 罗华1; 李良明1; 邓大成1
（1.邵阳市农业科学研究院，湖南 邵阳 422000；2.湖南雪峰种业有限责任公司，湖南 邵阳 422000）
Author(s):
SUN Bo1; 2; YI Baoyuan1; YANG Lianhua1; LUO Hua1; LI Liangming1; DENG Dacheng1
(1.Shaoyang Academy of Agricultural Sciences，Shaoyang,Hunan 422000；2.Hunan Xuefeng Seeds Co.Ltd.，Shaoyang,Hunan 422000)
关键词:
湖南; 西瓜; SSR标记; 指纹图谱; 遗传多样性
Keywords:
Hunan; watermelon; SSR marker; fingerprint; genetic diversity
DOI:
10.11937/bfyy.20223483
文献标志码:
A
摘要:
以湖南地区主栽西瓜品种中11份种质资源为试材，通过对23对核心SSR引物的筛选来构建指纹图谱，并进行遗传多样性分析，以期为湖南地区的西瓜品种保护、西瓜育种方向及种质资源创新提供参考依据。结果表明：仅用8对多态性引物便成功构建出11份西瓜品种的指纹图谱。聚类分析结果显示，大部分品种分子标记遗传分析与表型一致，但仍有部分品种与表型存在差异，推测可能是引物位点过少导致。在遗传距离0.66处，可将供试西瓜品种分为四大类。
Abstract:
Taking 11 germplasm resources of the main watermelon cultivars in Hunan as test material，the fingerprint was constructed by screening 23 pairs of core SSR primers，and the genetic diversity analysis was carried out，in order to provide reference for the protection of watermelon varieties，the direction of watermelon breeding and the innovation of germplasm resources in Hunan region.The results showed that only 8 pairs of polymorphic primers were used to construct the fingerprints of 11 watermelon varieties.The results of cluster analysis showed that the genetic analysis of molecular markers of most cultivars was consistent with phenotype，but there were still some varieties and phenotypes that differed，which was speculated to be caused by too few primer sites.At a genetic distance of 0.66，the watermelon varieties tested could be divided into four categories.

参考文献/References:

[1]佚名.2017年全国各地蔬菜、西瓜、甜瓜、草莓、马铃薯播种面积和产量[J].中国蔬菜,2019(11):22.[2]彭丁文,朱智勇,刘跃荣,等.南方主要两系不育系遗传多样性分析及指纹图谱构建[J].杂交水稻,2021,36(4):76-81.[3]杨扬,仇律雯,田红丽，等.308个糯玉米审定品种SSR标记遗传多样性分析[J].植物遗传资源学报,2022,23(1):169-176.[4]郑永胜,王丽媛,段丽丽，等.350份小麦种质的SSR遗传多样性分析[J].山东农业科学,2020,52(12):1-6.[5]GUO S，ZHANG J，SUN H，et al.The draft genome of watermelon (Citrullus lanatus) and resequencing of 20 diverse accessions[J].Nature Genetics，2013,45(1):51-58.[6]李超汉,宋荣浩,杨红娟，等.西瓜种质资源鉴定评价及其DNA指纹图谱构建[J].分子植物种,2021,19(8):2678-2689.[7]赵胜杰,朱红菊,路绪强,等.利用核心简单重复序列(SSR)标记分析无籽西瓜品种的DNA指纹及遗传多样性[J].农业生物技术报,2014,22(2):188-194.[8]范建光.基于SSR标记的西瓜DNA指纹图谱库的构建[D].泰安:山东农业大学,2013.[9]柳唐镜,李文信,洪日新,等.华南西瓜主要品种DNA指纹图谱构建研究(摘要)[J].中国瓜菜,2014,27(S1):81-82.[10]ZHANG H Y，WANG HUI，GUO G S,et al.Identification and validation of a core set of microsatellite markers for genetic diversity analysis in watermelon，Citrullus lanatus Thunb.Matsum.& Nakai[J].Euphytuca,2012,186:329-342.[11]刘泽发,孙小武,董亚静.SRAP标记鉴定西瓜种子纯度方法研究[J].中国瓜菜,2009,22(1):5-8.[12]张海英，许勇．十大技术之一（一）西瓜资源与品种核酸指纹鉴定关键技术[J].中国瓜菜，2013，26（5）：63-65．[13]NEI M，LI W H.Mathematical model for studying genetic variation interims of restriction Endonucleases[J].Proceeding of the National Academy Sciences of the United States of the America，1979，76：5269-5273.[14]徐彦刚,姚协丰,李良俊,等.西瓜核心种质遗传多样性分析及指纹图谱构建[J].分子植物育种,2021,19(1):305-317.[15]范建光,张海英,宫国义,等.西瓜DUS测试标准品种SSR指纹图谱构建及应用[J].植物遗传资源学报,2013,14(5):892-899.[16]王铭,刘江,王长彪,等.109份西瓜育种材料果实性状的遗传多样性分析[J].中国瓜菜,2020,33(10):23-28.[17]胡建斌,马肖静,李琼.薄皮甜瓜表型性状的主成分分析[J].江西农业学报,2010,22(12):30-33.[18]李清,郭禄芹,方晓霞,等.100份西瓜种质果实品质相关性状的遗传多样性分析[J].中国瓜菜,2019,32(1):12-17.[19]栾非时,闫令文,刘树森,等.西瓜种质资源背景选择标记筛选及遗传多样性分析[J].东北农业大学学报,2021,52(7):19-32.[20]高宁宁,李晓慧,康利允,等.基于SRAP分子标记的51份西瓜抗、感病毒病种质资源遗传多样性分析[J].南方农业学报,2021,52(5):1174-1182.[21]郭禄芹,赵世豪,朱华玉,等.167份西瓜种质材料的遗传多样性分析[J].中国瓜菜,2018,31(1):2,5-11.[22]ZHANG X P，RHODES B B，SKORUPSKA H.RAPD Molecular markers in watermelon[J].Hortscience，1993,28(5):223.[23]LEE S J，SHIN J S，PARK K W，et al.Detection of genetic diversity using RAPD-PCR and sugar analysis in watermelon [Citrullus lanantus (Thunb.) Mansf.] germplasm[J].Theor Appl Genet，1996，92(6)：719-725.

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备注/Memo

第一作者简介：孙波（1993-），男，硕士，农艺师，现主要从事蔬菜遗传育种及种子质量检测等研究工作。E-mail：1021331329@qq.com.责任作者：李良明（1968-），男，本科，高级农艺师，现主要从事土肥、作物育种及栽培等研究工作。E-mail：15802521340@163.com.基金项目：国家西甜瓜产业技术体系资助项目（CARS-25）；邵阳市小荷人才资助项目；邵阳市农业科学研究院人才托举资助项目（2020TJ02）。收稿日期：2022-08-25

更新日期/Last Update: 2023-06-27