JIANG Liangzhen,MA Jianhua,HUANG Peng,et al.Tissue Culture and Rapid Propagation of Tripterygium wilfordii Seedlings Based on Air Rooting[J].Northern Horticulture,2019,43(15):119-125.[doi:10.11937/bfyy.20184075]
基于气生根的雷公藤植株的快速繁殖技术
- Title:
- Tissue Culture and Rapid Propagation of Tripterygium wilfordii Seedlings Based on Air Rooting
- Keywords:
- Tripterygium wilfordii; tissue culture; rapid proliferation; air suspension rooting; plant hormones
- 文献标志码:
- A
- 摘要:
- 以雷公藤幼嫩茎段为外植体材料，研究雷公藤无菌体系的建立，植物生长调节剂以及培养方式对诱导腋芽萌发和茎段快速生根的影响，以期获得雷公藤植株的快速繁殖技术。结果表明：雷公藤外植体的最佳消毒方式是75% 酒精30 s+0.1%升汞10 min；腋芽萌发的最佳培养基为MS+1.5 mg?L-1 6-BA+0.2 mg?L-1 NAA，最佳培养条件为温度(23±1)℃，湿度70%±5%，光/暗培养12 h/12 h，腋芽的萌发率为98.3%；生根的最佳培养基为1/2 MS+0.2 mg?L-1 NAA+0.1 mg?L-1 IAA+0.03%活性炭，最佳培养条件为叶片支撑的带芽茎段空气悬浮培养，温度为(23±1)℃，湿度为70%±5%，光/暗培养12 h/12 h，不定根的萌发率达到91.5%。该空气生根的雷公藤体外培养体系方法简单，月增殖系数可达到3.8，可同时快速实现雷公藤植株的增殖培养和生根培养。
- Abstract:
- To develop new tissue culture and rapid propagation systems of Tripterygium wilfordii Hook.f.,the young shoots were used as explants to induce axillary buds germination.Germinated axillary buds were cut for root inducing and elongation of stems and leaves.The elongated stems were excised into budded stem segments,from which roots were induced in a specific culture medium to achieve rapid plant proliferation.The results showed that the best sterilization effects were achieved when the explants were soaked in 75% alcohol for 30 seconds,washed three times with sterile water,soaked in 0.1% mercury liter solution for 10 minutes.The most effective medium for axillary bud germination was MS medium with 1.5 mg?L-1 6-BA+0.2 mg?L-1 NAA.The efficacy could reach 98.3%.The optimum medium for inducing rapid rooting of stem segments was 1/2MS medium with 0.2 mg?L-1 NAA+0.1 mg?L-1 IAA+0.03% activated carbon.The optimal root-inducing condition was air suspension culture with the lower stem ends of ecology at about 1 cm height from the medium at (23±1)℃,and the humidity being 70%±5% by the 12 h/12 h light/dark culture.The germination efficacy of adventitious roots reached 91.5%.In this study,a tissue culture system for rapid propagation of Tripterygium wilfordii seedlings was established.With this simple method,the monthly proliferation coefficient could reach 3.8.Cutting and transferring budded stem segments to root-inducing medium could realize rapid proliferation of stems and leaves and rooting at the same time.
参考文献/References:
[1]涂育合.雷公藤栽培与利用[M].北京：中国农业出版社,2006.[2]刘为萍,刘素香,唐慧珠,等.雷公藤研究新进展[J].中草药,2010,41(7):1215-1218.[3]汪群红,胡敏.雷公藤的药理作用与毒副作用[J].中国药业,2010,19(19):85-86.[4]强春倩,刘世任,都本敏.雷公藤药理研究进展[J].中国中医急症,2006,15(2):108-109.[5]薛璟,贾晓斌,谭晓斌,等 雷公藤化学成分及其毒性研究进展[J].中华中医药杂志,2010(5):726-733.[6]LIU Q.Triptolide and its expanding multiple pharmacological functions[J].International Immunopharmacology,2011,11(3):377-383.[7]LI X J,JIANG Z Z,ZHANG L Y.Triptolide：Progress on research in pharmacodynamics and toxicology[J].J Ethnopharmacol，2014,155(1):67-79.[8]李建友,夏素霞,宋少江.雷公藤二萜类化学成分及在研新药的研究进展[J].沈阳药科大学学报,2012(11):901-908.[9]卞晓霞.雷公藤红素药理作用研究进展[J].中国当代医药,2009,16(13):18-19.[10]任献青,鲁静,孟祥乐,等 雷公藤红素药理作用最新研究进展[J].中华中医药杂志,2013(9):2679-2682.[11]林照授,田有圳,涂育合,等.雷公藤苗木的繁育方法[J].林业科技通讯,2013(4):23-25.[12]曾黎辉,吕柳新.木本果树遗传转化研究进展[J].果树学报,2002,19(3):191-198.[13]赖呈纯,余亚白,谢鸿根,等.雷公藤组织培养快繁技术初探[J].武夷科学,2006,22(1):182-184.[14]刘希华,曾淑兰,丁昌俊,等.雷公藤以芽繁芽组织培养研究[J].西南林业大学学报,2009,29(1):35-38.[15]李琰，冯俊涛，陈新雨,等.雷公藤胚性愈伤组织再生植株的增殖及其稳定性[J].林业科学,2009,45(1):57-61.[16]陈凌艳,陈拓,荣俊冬,等.雷公藤组织培养快速繁殖技术研究[J].江苏林业科技,2016,43(2):8-12.[17]孙乙铭,徐建中,王志安,等.HPLC测定不同采收年限雷公藤根及不同部位雷公藤甲素含量[J].中国现代应用药学,2009(11):904-906.
相似文献/References:
[1]王海新,赵艳,尤淑丽,等.植物生长调节剂“易丰收”对树莓组培苗生根的影响[J].北方园艺,2013,37(04):31.
[2]陶米林,刘清波,黄红梅,等.半夏组织培养体系的建立[J].北方园艺,2013,37(04):113.
[3]曹 虹,金荣荣.三倍体薄皮甜瓜未成熟胚子叶诱导不定芽的研究[J].北方园艺,2014,38(13):106.
CAO Hong,JIN Rong-rong.Study on Adventitious Bud From Immature Embryo Cotyledon of Triploid Muskmelon[J].Northern Horticulture,2014,38(15):106.
[4]张振霞,李彩萍,张秋枚,等.甘肃贝母的组织培养和植株再生研究[J].北方园艺,2014,38(10):80.
ZHANG Zhen-xia,LI Cai-ping,ZHANG Qiu-mei,et al.Study on Tissue Culture and Plant Regeneration of Fritillaria przewalskii Maxim[J].Northern Horticulture,2014,38(15):80.
[5]王玉娇,杨 忠,赵春莉,等.兴安白头翁组织培养与快繁技术研究[J].北方园艺,2014,38(10):84.
WANG Yu-jiao,YANG Zhong,ZHAO Chun-li,et al.Study on Tissue Culture and Rapid Propagation of Pulsatill dahurica（Fisch.）Spreng[J].Northern Horticulture,2014,38(15):84.
[6]朱 军,李晓瑾,孙 丽,等.药用植物玛咖离体快繁技术研究[J].北方园艺,2013,37(22):101.
ZHU Jun,LI Xiao-jin,SUN Li,et al.Study on the Rapid Propagation of Medicinal Plants Lepidium meyenii[J].Northern Horticulture,2013,37(15):101.
[7]杜 丽.香樟子叶胚培养的研究[J].北方园艺,2014,38(14):89.
DU Li.Research on Cotyledonary Embryo Culture of Cinnamomum camphora L.[J].Northern Horticulture,2014,38(15):89.
[8]赵丽莉,徐芳芳,荀 洋,等.桔梗花药愈伤组织诱导培养基筛选[J].北方园艺,2014,38(01):95.
ZHAO Li-li,XU Fang-fang,XUN Yang,et al.Screening of Callus Induction Medium of Platycodon grandiflorumAnther[J].Northern Horticulture,2014,38(15):95.
[9]李云玲,孙 虎,苗锦山,等.黄山贡菊茎尖脱毒快繁技术研究[J].北方园艺,2014,38(01):106.
LI Yun-ling,SUN Hu,MIAO Jin-shan,et al.Tissue Culture and Rapid Propagation of Chrysanthemum morifolium[J].Northern Horticulture,2014,38(15):106.
[10]陈丽文,杨利平,荣薏,等.金心也门铁工厂化育苗技术研究[J].北方园艺,2013,37(13):138.
CHEN Liwen,YANG Li-ping,RONG Yi,et al.Study on the Techniques of Industrialized Seedling Culture of Draceana arborea[J].Northern Horticulture,2013,37(15):138.
备注/Memo
第一作者简介：姜良珍(1987-)，女，山东临沂人，博士，讲师，研究方向为药用植物分子生物学。E-mail：lzhjiang1987@sina.com.责任作者：赵琦（1981-），男，河北满城人，博士，副教授，研究方向为药用植物分子生物学。E-mail：zhaoqi@cdu.edu.cn.基金项目：成都大学人才工程基金资助项目（2081918009）；成都大学校青年基金资助项目（2080518011）。收稿日期：2019-03-06