MA Jingui,GUO Shuying.Tissue Culture Technique for Weigela ‘Red Prince’+vat[J].Northern Horticulture,2017,41(10):92-94.[doi:10.11937/bfyy.201710021]
金叶锦带组培技术
- Title:
- Tissue Culture Technique for Weigela ‘Red Prince’+vat
- Keywords:
- Weigela‘Red Prince’+vat; tissue culture; hormone combination
- 文献标志码:
- A
- 摘要:
- 以金叶锦带的腋芽为外植体，采用组织培养法，通过在培养基中添加不同浓度激素，筛选最佳培养基，为金叶锦带的大面积推广提供技术支撑。结果表明：最佳诱导培养基为MS+0.6 mg?L-1 6-BA+0.1 mg?L-1 NAA+蔗糖30 g?L-1+琼脂7 g?L-1，诱导率达86.3%；最佳增殖培养基为MS+1.0 mg?L-1 6-BA+1.0 mg?L-1 GA3+〖JP〗蔗糖30 g?L-1+琼脂7 g?L-1，增殖系数7.250 6；最佳生根培养基为1/2MS+1.0 mg?L-1 IBA+蔗糖30 g?L-1+琼脂7 g?L-1，生根率100.0%。
- Abstract:
- Buds of Weigela ‘Red Prince’+vat were used as explants.By using tissue culture methods，the optimum medium for Weigela ‘Red Prince’+vat were selected via adding different concentrations of hormone,to provide technique reference for promotion of Weigela‘Red Prince’+vat.The results showed that the inducing culture medium of MS+0.6 mg?L-1 6-BA+0.1 mg?L-1 NAA+sucrose 30 g?L-1+agar 7 g?L-1 was optimal for inducement，the inducement rate was 86.3%;the best proliferation medium was MS+1.0 mg?L-1 6-BA+1.0 mg?L-1 GA3+sucrose 30 g?L-1+agar 7 g?L-1，the proliferation times was 7.250 6;the best rooting medium formula was 1/2MS+1.0 mg?L-1 IBA+sucrose 30 g?L-1+agar 7 g?L-1,the rooting rate was 100.0%.
参考文献/References:
[1]翟咏,胡二红.金叶锦带在太原地区的繁殖及推广[J].山西科技,2014(6):158-159. [2]李久亮,苑兆和,招雪晴,等.加拿大紫荆‘森林火焰’组培快繁技术研究[J].中国农学通报,2011(6):64-67. [3]张爽,梁本国.植物组织培养[M].武汉：华中科技大学出版社,2013:52-53.
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备注/Memo
第一作者简介：马金贵(1964-)，男，河北丰润人，硕士，副教授，现主要从事园林植物栽培教学与研究等工作。E-mail:tsguoshuying@163.com.责任作者：郭淑英（1964-）,女，本科，教授，现主要从事园林植物栽培与应用教学与研究等工作。基金项目：河北省科技厅科学研究计划资助项目（15236830）。