[1]朱强龙,赵玉龙,吕慧玲,等.西瓜CBL家族基因的鉴定与特征分析[J].北方园艺,2017,41(15):18-24.[doi:10.11937/bfyy.20170580]
　ZHU Qianglong,ZHAO Yulong,LYU Huiling,et al.Identification and Characterization of CBL Family Genes in Watermelon [J].Northern Horticulture,2017,41(15):18-24.[doi:10.11937/bfyy.20170580]
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西瓜CBL家族基因的鉴定与特征分析

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第41卷 期数: 2017年15 页码: 18-24 栏目: 出版日期: 2017-08-15
Title:
Identification and Characterization of CBL Family Genes in Watermelon 
作者:
朱强龙; 赵玉龙; 吕慧玲; 栾非时; 高鹏
（东北农业大学 园艺园林学院，农业部东北地区园艺作物生物学与种质创制重点实验室，黑龙江 哈尔滨 150030）
Author(s):
ZHU Qianglong; ZHAO Yulong; LYU Huiling; LUAN Feishi; GAO Peng
(College of Horticulture and Landscape Architecture，Northeast Agricultural University/Key Laboratory of Biology and Genetic Improvement of Horticulture Crops (Northeast region)，Ministry of Agriculture，Harbin，Heilongjiang 150030)
关键词:
西瓜; CBL基因; 分子结构特征; 蛋白结构域; 遗传多样性聚类; 顺式元件
Keywords:
watermelon(Citrullus lanatus); CBL gene; sequence characterization; protein structure; phylogeny; cis-elements
DOI:
10.11937/bfyy.20170580
文献标志码:
A
摘要:
CBL基因在植物逆境应答过程中具有重要作用，但在西瓜作物的抗逆育种研究中鲜有相关报道。该研究利用生物信息学的分析方法从已发表的西瓜全基因组中鉴定出7个CBL基因（ClaCBL1~ClaCBL7〖STBZ〗），并分析了它们在全基因组范围内的分布情况、分子结构特征、顺式元件以及系统进化等基本特征，为进一步研究西瓜CBL基因的功能提供参考。结果表明：西瓜CBL基因在全基因组中的分布是不均匀的，其中仅ClaCBL4〖STBZ〗基因有9个外显子，其余基因均为8个外显子，编码区序列大小在639～738 bp。在进化上西瓜CBL基因分为3个不同的类群；它们编码区域内的氨基酸序列均含有能与CIPK互作的FSPF高保守性位点及能与钙离子结合的3个EF-hand结构；除了ClaCBL1预测定位在胞外基质中，其余的ClaCBL定位在细胞内的不同部位；在西瓜CBL基因启动子上游的序列中存在多个应答不同逆境和植物激素的顺式元件，而且CBL家族不同成员基因含有的顺式元件的种类和数目各不相同。上述分析显示，西瓜CBL可能参与多种生物学过程，而且不同成员存在功能上的分化。
Abstract:
Plant CBL family genes play important roles in response to environmental stimuli.However,the available knowledge of CBL in watermelon is insufficient.In this study,seven CBL genes (ClaCBL1-ClaCBL7) 〖STBZ〗were annotated in watermelon genome by bioinformatics method and their genomic distribution,molecular feature,phylogeny and cis-elements were also analyzed for providing valuable information for further functional dissection of watermelon CBL genes.The results showed that watermelon CBL genes were unevenly distributed in genome,and only one (ClaCBL4〖STBZ〗) had nine exons and the rest of them had eight exons.The putative coding sequence length of CBL ranges from 639 bp to 738 bp.On the basis of phylogeny,they were divided into three groups.Their coded proteins had FPSF motif，which interacted with CIPK kinases，and three EF-hand motifs，which could bind calcium ions.The putative proteins of ClaCBL were all localized into different subcellular locations except ClaCBL1 in extracellular matrix.In addition,there are several cis-elements existed in upstream sequences of watermelon CBL genes,which could response to different environmental stimuli and phytohormones.And the cis-element types and number of different watermelon CBL genes were not identical.These results indicated that watermelon CBL genes might involve in multiple biological processes and they had different functions.

参考文献/References:

[1]LUAN S.The CBL-CIPK network in plant calcium signaling[J].Trends Plant Sci,2009(1):37-42.

[2]SANCHEZ-BARRENA M J,MARTINEZ-RIPOLL M,ALBERT A.Structural biology of a major signaling network that regulates plant abiotic stress:The CBL-CIPK mediated pathway[J].Int J Mol Sci,2013(3):5734-5749.

[3]KIM K N.Stress responses mediated by the CBL calcium sensors in plants[J].Plant Biotechnol Rep,2013(1):1-8.

[4]PANDEY G K,CHEONG Y H,KIM K N,et al.The calcium sensor calcineurin B-like 9 modulates abscisic acid sensitivity and biosynthesis in Arabidopsis[J].Plant Cell,2004(7):1912-1924.

[5]CHEONG Y H,KIM K N,PANDEY G K,et al.CBL1,a calcium sensor that differentially regulates salt,drought,and cold responses in Arabidopsis[J].Plant Cell,2003(8):1833-1845.

[6]LI Z Y,XU Z S,CHEN Y,et al.A novel role for Arabidopsis CBL1 in affecting plant responses to glucose and gibberellin during germination and seedling development[J/OL].PLoS One,2013(2):e56412.

[7]FUGLSANG A T,GUO Y,CUIN T A,et al.Arabidopsis protein kinase PKS5 inhibits the plasma membrane H+-ATPase by preventing interaction with 14-3-3 protein[J].Plant Cell,2007(5):1617-1634.

[8]TANG R J,ZHAO F G,GARCIA V J,et al.Tonoplast CBL-CIPK calcium signaling network regulates magnesium homeostasis in Arabidopsis[J].Proc Natl Acad Sci USA,2015(10):3134-3139.

[9]ECKERT C,OFFENBORN J N,HEINZ T,et al.The vacuolar calcium sensors CBL2 and CBL3 affect seed size and embryonic development in Arabidopsis thaliana[J].Plant J,2014(1):146-156.

[10]TANG R J,LIU H,YANG Y,et al.Tonoplast calcium sensors CBL2 and CBL3 control plant growth and ion homeostasis through regulating V-ATPase activity in Arabidopsis[J].Cell Res,2012(12):1650-1665.

[11]MAO J,MANIK S M N,SHI S,et al.Mechanisms and physiological roles of the CBL-CIPK networking system in Arabidopsis thaliana[J].Genes,2016,7(9):62.

[12]QUAN R,LIN H,MENDOZA I,et al.SCABP8/CBL10,a putative calcium sensor,interacts with the protein kinase SOS2 to protect Arabidopsis shoots from salt stress[J].Plant Cell,2007(4):1415-1431.

[13]JI H,PARDO J M,BATELLI G,et al.The salt overly sensitive (SOS) pathway:Established and emerging roles[J].Mol Plant,2013(2):275-286.

[14]CHEONG Y H,SUNG S J,KIM B G,et al.Constitutive overexpression of the calcium sensor CBL5 confers osmotic or drought stress tolerance in Arabidopsis[J].Mol Cells,2010(2):159-165.

[15]XU J,LI H D,CHEN L Q,et al.A protein kinase,interacting with two calcineurin B-like proteins,regulates K+ transporter AKT1 in Arabidopsis[J].Cell,2006(7):1347-1360.

[16]KOLUKISAOGLU U,WEINL S,BLAZEVIC D,et al.Calcium sensors and their interacting protein kinases:Genomics of the Arabidopsis and rice CBL-CIPK signaling networks[J].Plant Physiol,2004(1):43-58.

[17]GU Z,MA B,JIANG Y,et al.Expression analysis of the calcineurin B-like gene family in rice (Oryza sativa L.) under environmental stresses[J].Gene,2008(1-2):1-12.

[18]WANG M,GU D,LIU T,et al.Overexpression of a putative maize calcineurin B-like protein in Arabidopsis confers salt tolerance[J].Plant Mol Biol,2007(6):733-746.

[19]李利斌,刘开昌,王殿峰,等.玉米CBL基因的生物信息学分析[J].玉米科学,2010(1):6-11.

[20]MAHAJAN S,SOPORY S K,TUTEJA N.Cloning and characterization of CBL-CIPK signalling components from a legume (Pisum sativum)[J].FEBS J,2006(5):907-925.

[21]李利斌,刘开昌,王殿峰,等.高粱CBL家族基因的鉴定和初步分析[J].山东农业科学,2009(6):1-5.

[22]李利斌,王殿峰,刘立锋,等.大白菜CBL家族基因的鉴定和遗传进化分析[J].山东农业科学,2009(5):4-7.

[23]张永涛,刘立峰,李化银,等.三个新的大白菜CBL基因的鉴定和特征分析[J].山东农业科学,2012(12):7-10.

[24]刘淑梅,王施慧,刘明毓,等.番茄CBL家族基因的鉴定和遗传进化分析[J].分子植物育种,2015(10):2268-2273.

[25]刘明毓,纪复勤,马强,等.甜瓜CBL基因的鉴定和特征分析[J].山东农业科学,2015(10):17-21.

[26]FAN M,HUANG Y,ZHONG Y,et al.Comparative transcriptome profiling of potassium starvation responsiveness in two contrasting watermelon genotypes[J].Planta,2014(2):397-410.

[27]HU B,JIN J,GUO A Y,et al.GSDS 2.0:An upgraded gene feature visualization server[J].Bioinformatics,2015(8):1296-1297.

[28]TAMURA K,STECHER G,PETERSON D,et al.MEGA6:Molecular evolutionary genetics analysis version 6.0[J].Mol Biol Evol,2013(12):2725-2729.

[29]HORTON P,PARK K J,OBAYASGI T,et al.WoLF PSORT:Protein localization predictor[J].Nucleic Acids Res,2007(Web Server issue):585-587.

[30]EMANUELSSON O,BRUNAK S,VON H G,et al.Locating proteins in the cell using TargetP,SignalP and related tools[J].Nat Protoc,2007(4):953-971.

[31]LESCOT M,DHAIS P,THIJS G,et al.PlantCARE,a database of plant cis-acting regulatory elements and a portal to tools for in silico analysis of promoter sequences[J].Nucleic Acids Res,2002(1):325-327.

[32]THODAY - KENNEDY E L,JACOBS A K,ROY S J.The role of the CBL - CIPK calcium signalling network in regulating ion transport in response to abiotic stress[J].Plant Growth Regul,2015,76(1):3 - 12.

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备注/Memo

第一作者简介：朱强龙（1990-），男，博士研究生，研究方向为西瓜分子遗传育种。E-mail：longzhu2011@126.com.责任作者：高鹏（1982-），男，博士，副研究员，硕士生导师，现主要从事西甜瓜分子遗传育种等研究工作。E-mail：gaopeng_neau@163.com.基金项目：国家自然科学基金面上资助项目（31572144）；国家西甜瓜产业技术体系-分子育种岗位资助项目（CARS-26-02）。

收稿日期：2017-03-30

更新日期/Last Update: 2017-08-03