QI Haijun,GAO Hui,CHEN Fenfen,et al.The Optimum Callus Induction Media for Panax ginseng Leaf Callus Induction by Using Uniform Design[J].Northern Horticulture,2016,40(14):106-109.[doi:10.11937/bfyy.201614026]
均匀设计法优化人参叶片愈伤组织诱导培养基
- Title:
- The Optimum Callus Induction Media for Panax ginseng Leaf Callus Induction by Using Uniform Design
- Keywords:
- Panax ginseng leaf; callus; uniform design; optimum media
- 文献标志码:
- A
- 摘要:
- 以人参叶片为试材，MS为基本培养基，采用均匀设计和DPS软件分析的方法，研究了2,4-D、KT、NAA、6-BA等4种不同激素组合对人参愈伤组织诱导的影响，以期筛选出诱导人参叶片愈伤组织的最佳激素组合。结果表明：人参叶片愈伤组织诱导的最佳培养基为MS+2,4-D 2 mg?L-1+NAA 0.66 mg?L-1+KT 1.0 mg?L-1。验证试验表明，在添加最佳激素组合的MS培养基中接种新的叶片外植体，30 d后愈伤组织诱导率为93%，单个叶片外植体上愈伤组织小块平均数达1.82.
- Abstract:
- Taking Panax ginseng leaf as material，with MS as basic medium，the effects of 2,4-D，KT，NAA and 6-BA on callus induction were tested by using the uniform design.Then using the DPS software analyzed the data and find out the optimum callus induction media for callus induction.The results showed that the optimum induction media was MS+2，4-D 2 mg?L-1+NAA 0.66 mg?L-1+KT 1.0 mg?L-1.Verification test indicated the callus induction rate was up to 93% after 30 days culture on the optimum media，and the callus average value on single leaf explant was 1.82.
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备注/Memo
第一作者简介：齐海军（1989-），男，山西孝义人，硕士研究生，研究方向为生物技术在作物遗传育种中的应用。E-mail：649052084@qq.com.责任作者：金东淳（1963-），男，吉林图们人，博士，副教授，研究方向为生物技术在作物遗传育种中的应用。E-mail：jdc1200@ybu.edu.cn.