YANG Huanhuan,JIANG Jingbin,LI Jingfu,et al.Rapid Detection of Tomato Yellow Leaf Curl Virus Resistant Gene ty-5 in Tomato by PCR[J].Northern Horticulture,2016,40(14):110-114.[doi:10.11937/bfyy.201614027]
番茄抗黄化曲叶病ty-5基因的PCＲ快速检测
- Title:
- Rapid Detection of Tomato Yellow Leaf Curl Virus Resistant Gene ty-5 in Tomato by PCR
- Keywords:
- tomato yellow leaf curl virus; ty-5; SSR; PCR
- 文献标志码:
- A
- 摘要:
- 以番茄抗病材料CLN32120a-23（含ty-5 基因）和感病材料Moneymaker（不含抗病基因）为试材，通过杂交、自交获得的F1、F2代，利用SSR分子标记技术筛选出了1个与抗病性状共分离的共显性SSR标记。结果表明：该标记在CLN32120a-23中可以扩增出550 bp的条带，在Moneymaker中可以扩增出780 bp的条带，在F1中可以扩增出上述2条条带，标记结果与田间鉴定基本一致，证明该标记能够区分抗病材料、感病材料及杂合抗病材料，是与番茄抗黄化曲叶病毒病基因ty-5紧密连锁的共显性标记。对27份番茄材料进行检测，鉴定出有15份材料基因型为ty-5/ty-5，分子标记检测与田间检测结果吻合率达80%。该标记可用于番茄抗黄化曲叶病毒ty-5基因的PCR快速检测，同时为ty-5分子标记辅助选择育种的应用提供一定的参考依据。
- Abstract:
- Taking CLN32120a-23（resistant materials）and Moneymaker（susceptible materials）as experimental materials which is resistant to yellow leaf curl virus in tomato，F1、F2 were obtained through hybridization，selfcross，using SSR technology，a molecular marker was obtained.The results showed that SSR marker had a 550 bp amplification fragment in homozygous resistant lines，a 780 bp fragment in susceptible lines and both 780 bp and 550 bp fragments in heterozygous resistant lines.The detection results were consistent with those of field identification.So the SSR marker，tightly linked to ty-5 gene，could distinguish homozygous and heterozygous resistant lines and susceptible lines.27 tomato materials individuals were detected.There were 15 homozygous resistant lines，the molecular detection was 80% coincident with the results of field identification.The results showed that ty-5 SSR marker could be used for rapid identification of ty-5 resistant gene in tomato.
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备注/Memo
第一作者简介：杨欢欢（1988-），女，博士研究生，研究方向为蔬菜育种。E-mail:huanyaya0126@sina.com.责任作者：李景富（1943-），男，博士，教授，博士生导师，现主要从事番茄种质资源的研究与利用等工作。E-mail：Lijf_2005@126.com.基金项目：国家自然科学基金资助项目（31272171）；黑龙江省杰出青年科学基金资助项目（JC201204）；“十二五”国家科技支撑资助项目（2012BAD02B02-7）。