LI Sen,GAO Lixia,LIU Nian,et al.Tissue Culture of Vaccinium corymbosum ‘O’Neal’[J].Northern Horticulture,2016,40(09):102-105.[doi:10.11937/bfyy.201609027]
高丛越橘“奥尼尔”的组织培养
- Title:
- Tissue Culture of Vaccinium corymbosum ‘O’Neal’
- 关键词:
- 高丛越橘 “奥尼尔”; 组织培养; 增殖
- Keywords:
- Vaccinium corymbosum ‘O’Neal’; tissue culture; proliferation
- 文献标志码:
- A
- 摘要:
- 以高丛越橘“奥尼尔”的带芽茎段为外植体，采用组织培养技术，研究了不同配比的ZT、6-BA、NAA和IBA植物激素对高丛越橘“奥尼尔”外植体诱导的影响。结果表明：最佳的外植体消毒条件为0.1% HgCl2 4 min，萌芽率可达71.4%。腋芽诱导的初代培养宜用WPM+1.0 mg/L ZT培养基，侧枝能够萌发，苗高4.7 cm；适宜的增殖培养基为WPM+2.0 mg/L ZT，增殖系数达到13.0；试管苗的生根培养基为1/2WPM+0.1 mg/L IBA。当试管苗长至5 cm出瓶移栽，成活率可达80%以上。
- Abstract:
- Taking stems with buds of Vaccinium corymbosum ‘O’Neal’ as explants，the effect of different combination of ZT，6-BA，NAA and IBA added into the media were tested.The results showed that the optimal disinfection treatment was soaking into 0.1% HgCl2 for 4 min with induction rate of 71.4%.The optimal medium for bud induction was WPM+1.0 mg/L ZT on which some branches germinated and plantlet length reached to be 4.7 cm.The suitable proliferation medium was WPM+2.0 mg/L ZT and the proliferation coefficient achieved to be 13.0.The optimum medium for root induction was 1/2WPM+0.1 mg/L IBA.The plantlets reached to be 5 cm in length and could be transplanted outside and the survival rate exceeded 80%.
参考文献/References:
[1]苑兆和.世界蓝莓生产历史与发展趋势[J].落叶果树，2003,20(1):49-52. [2]刘庆忠，赵红军.越桔高效栽培与加工利用[M].北京：中国农业出版社，2003：64-71. [3]张长青,李广平,朱士农,等.兔眼越橘茎段快繁高效技术研究[J].果树学报，2007,24(6):837-840. [4]刘树英，安伟，孔令学，等.兔眼越橘芽的诱导及再生[J].吉林农业大学学报,2004,26(6):632-635. [5]黄文江,刘庆忠,阚显照.高灌蓝莓离体繁殖的研究[J].安徽师范大学学报(自然科学版),2004,27(3):314-317. [6]朱宏芬,沈岚,黄坚,等.兔眼蓝莓“灿烂”组织培养与植株再生研究[J].北方园艺,2012(19):105-107. [7]李丽容,金开正.兔眼蓝莓组培快繁试验[J].中国南方果树,2010,39(1):71-72. [8]张力思,魏海蓉,艾呈祥,等.培养基组分对蓝莓组培增殖效率的影响[J].落叶果树,2006,38(4):13-14. [9]刘庆忠,赵红军.高灌蓝莓的组织培养及快速繁殖[J].植物生理学通讯，2002,38(3):253. [10]宁志怨,江芹,陈静娴,等.蓝莓丛生芽的诱导及植株再生[J].分子植物育种，2007,5(Z1):64-66.
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备注/Memo
第一作者简介：李森（1982-），男，硕士，助理研究员，现主要从事园艺植物良种繁育与商品化生产研究及科研管理等工作。E-mail：88045361@qq.com. 责任作者：张施君（1974-），女，博士，副研究员，现主要从事园艺植物育种与栽培研究及教学等工作。E-mail：guanyehuazu@163.com. 基金项目：广东省科技计划资助项目（2013B020304006）；广东省科技厅产学研合作资助项目（2013B090200065）；广东省科技计划资助项目（广东省落叶果树工程技术研究中心）（2015B090903082）。