WANG Daqing,ZHANG Jiao.Study on the Establishment of Regeneration System of Spiraea×bumalda[J].Northern Horticulture,2016,40(08):89-92.[doi:10.11937/bfyy.201608025]
绣线菊再生体系的建立
- Title:
- Study on the Establishment of Regeneration System of Spiraea×bumalda
- 文献标志码:
- A
- 摘要:
- 以金山绣线菊的茎尖、茎段、叶片、叶柄为外植体，以MS培养基为基本培养基，在初代、继代增殖和生根培养等不同阶段通过添加不同种类和浓度的激素进行对比试验，建立了适宜金山绣线菊组织培养的再生体系。结果表明：诱导愈伤组织培养基为：MS+1.0 mg/L 6-BA+0.1 mg/L NAA+2.0 mg/L 2,4-D，愈伤组织诱导不定芽培养基为：MS+0.8 mg/L TDZ+0.10 mg/L NAA，生根培养基为：1/2MS+0.25 mg/L 6-BA+1.0 mg/L NAA，腋芽增殖培养基为：MS+2.0 mg/L 6-BA+0.2 mg/L NAA。
- Abstract:
- The stem apex，stem，leaves and petiole of Spiraea×bumalda ‘Golden Mound’ were taken as explants for tissue culture in vitro.Different species and concentrations of hormones were added to MS medium during the different periods of first，subculture and rooting culture for establishing of regeneration system suitable for tissue culture of Spiraea×bumalda ‘Golden Mound’.The results showed that，medium for callus induction was MS+1.0 mg/L 6-BA+0.1 mg/L NAA+2.0 mg/L 2,4-D，medium for adventitious bud induction was MS+0.8 mg/L TDZ+0.10 mg/L NAA，medium for rooting was 1/2MS+0.25 mg/L 6-BA+1.0 mg/L NAA，medium for axillary bud proliferation was MS+2.0 mg/L 6-BA+0.2 mg/L NAA.
参考文献/References:
[1]赵冰,张欣欣.粉花绣线菊的组织培养与快繁技术[J].北方园艺,2010(13):157-159. [2]李晶,孙海滨,刘强.不同因子对华北绣线菊离体培养启动的影响[J].中国林副特产,2010(5):25-27. [3]徐倩.华北绣线菊引种繁育研究[D].青岛:青岛农业大学,2007. [4]陈发棣,腾年军,房伟民,等.三个菊花品种花器官愈伤组织辐射效应的研究[J].中南林学院学报,2003(5):27-29. [5]李云峰,王玉贤.日本绣线菊的组织培养[J].植物生理学通讯,1987(2):51. [6]胡益明,甘烦远,彭丽萍,等.星花绣线菊的组织培养及快速繁殖[J].植物生理学通讯,200l(3):235-236. [7]赵沛基,甘烦远,沈月毛.椭圆叶绣线菊的组织培养[J].植物生理学通讯,2004(1):71. [8]胡益明,甘烦远,彭丽萍,等.药用植物粉花绣线菊的组织培养[J].中草药,2001，32(11):1030-1033. [9]萤雅茹,王瑞库,王丽艳.石刁柏、珍珠梅、柳叶绣线菊腋芽茎段的离体培养[J].黑龙江八一农垦大学学报,1994(4):23-26. [10]张立磊,李保印,郭巧玲,等.彩叶植物金叶绣线菊组织培养研究[J].经济林研究,2005(1):47-49. [11]刘慧民,仉茜,苏青,等.18种绣线菊苗期抗寒性评价与筛选[J].园艺学报,2014，41(12):2427-2436. [12]冯楠楠.6种绣线菊抗寒能力的比较研究[D].哈尔滨:东北农业大学,2008. [13]杨玉想.盐碱地区金山绣线菊的繁殖与栽培技术[J].河北林业科技,2005(6):51. [14]周春华,尤超,陈凝华.百合组织培养研究进展[J].北方园艺,2013(14):193-195. [15]JACOBSSON S,KARLTORP K.Micropropagation of wild cherry K-I[J].Rastenievdni Nauki,1995(7/8):97-98. [16]薛银芳,赵大球,周春华,等.芍药组织培养的研究进展[J].北方园艺,2012(4):167-170. [17]李厚华,阙怡,费昭雪,等.红肉苹果组织培养及转基因体系的建立与优化[J].北方园艺,2011(15):175-179. [18]郭风云.芍药组织培养技术的研究[D].北京:北京林业大学,2001. [19]贾海慧.影响樱桃试管苗玻璃化的因素与克服措施[J].山东林业科技,2010(4):72-73. [20]任东植,李峰,曲云琴.影响枣组培苗玻璃化的几个因素及其防治[J].植物生理学通讯,2000(1):21-23.
相似文献/References:
[1]王海新,赵艳,尤淑丽,等.植物生长调节剂“易丰收”对树莓组培苗生根的影响[J].北方园艺,2013,37(04):31.
[2]陶米林,刘清波,黄红梅,等.半夏组织培养体系的建立[J].北方园艺,2013,37(04):113.
[3]曹 虹,金荣荣.三倍体薄皮甜瓜未成熟胚子叶诱导不定芽的研究[J].北方园艺,2014,38(13):106.
CAO Hong,JIN Rong-rong.Study on Adventitious Bud From Immature Embryo Cotyledon of Triploid Muskmelon[J].Northern Horticulture,2014,38(08):106.
[4]张振霞,李彩萍,张秋枚,等.甘肃贝母的组织培养和植株再生研究[J].北方园艺,2014,38(10):80.
ZHANG Zhen-xia,LI Cai-ping,ZHANG Qiu-mei,et al.Study on Tissue Culture and Plant Regeneration of Fritillaria przewalskii Maxim[J].Northern Horticulture,2014,38(08):80.
[5]王玉娇,杨 忠,赵春莉,等.兴安白头翁组织培养与快繁技术研究[J].北方园艺,2014,38(10):84.
WANG Yu-jiao,YANG Zhong,ZHAO Chun-li,et al.Study on Tissue Culture and Rapid Propagation of Pulsatill dahurica（Fisch.）Spreng[J].Northern Horticulture,2014,38(08):84.
[6]朱 军,李晓瑾,孙 丽,等.药用植物玛咖离体快繁技术研究[J].北方园艺,2013,37(22):101.
ZHU Jun,LI Xiao-jin,SUN Li,et al.Study on the Rapid Propagation of Medicinal Plants Lepidium meyenii[J].Northern Horticulture,2013,37(08):101.
[7]杜 丽.香樟子叶胚培养的研究[J].北方园艺,2014,38(14):89.
DU Li.Research on Cotyledonary Embryo Culture of Cinnamomum camphora L.[J].Northern Horticulture,2014,38(08):89.
[8]赵丽莉,徐芳芳,荀 洋,等.桔梗花药愈伤组织诱导培养基筛选[J].北方园艺,2014,38(01):95.
ZHAO Li-li,XU Fang-fang,XUN Yang,et al.Screening of Callus Induction Medium of Platycodon grandiflorumAnther[J].Northern Horticulture,2014,38(08):95.
[9]李云玲,孙 虎,苗锦山,等.黄山贡菊茎尖脱毒快繁技术研究[J].北方园艺,2014,38(01):106.
LI Yun-ling,SUN Hu,MIAO Jin-shan,et al.Tissue Culture and Rapid Propagation of Chrysanthemum morifolium[J].Northern Horticulture,2014,38(08):106.
[10]陈丽文,杨利平,荣薏,等.金心也门铁工厂化育苗技术研究[J].北方园艺,2013,37(13):138.
CHEN Liwen,YANG Li-ping,RONG Yi,et al.Study on the Techniques of Industrialized Seedling Culture of Draceana arborea[J].Northern Horticulture,2013,37(08):138.
备注/Memo
第一作者简介：王大庆（1969-），男，博士，研究员，现主要从事农业经济等研究工作。E-mail:183189637@qq.com.