LI Lixia,LI Tingting,ZHU Hong,et al.Different Factors on Callus Induction of Panax japonicus var.major[J].Northern Horticulture,2016,40(07):85-88.[doi:10.11937/bfyy.201607022]
濒危植物珠子参愈伤组织诱导因素分析
- Title:
- Different Factors on Callus Induction of Panax japonicus var.major
- Keywords:
- Panax japonicus var.major; tissue culture; callus
- 文献标志码:
- A
- 摘要:
- 以珠子参节、节间和越冬芽萌发后的叶、根为试材，研究了不同消毒方法、外植体、植物生长调节剂及培养条件等对珠子参愈伤组织诱导的影响，以期获得愈伤组织诱导的最佳方法。结果表明：最佳消毒方法是75%酒精消毒15 s后，越冬芽、节和节间分别加0.2%氯化汞消毒5、15、15 min；节间为愈伤组织诱导最适宜外植体；2,4-D 2.0 mg/L+KT 0.1 mg/L对节间诱导愈伤组织效果最好，诱导率为96.67%；以MS为基础培养基添加浓度为5 g/L的琼脂较适宜愈伤组织的诱导；温度25℃、光周期24 h/d、光照强度6 200 lx的条件有利于愈伤组织的诱导。
- Abstract:
- Taking knobs，internode and leaves，roots of winter buds of Panax japonicus var.major as experiment material，the effects of different disinfection method，explants，plant growth regulator and culture conditions on callus induction were studied to find the best method of callus induction.The results showed that the better disinfection treatment was 15 seconds with 75% C2H5OH，then 5 minutes with 0.2% HgCl2 for winter bud，but 15 minutes for knobs and internode.The internode was the best explant for callus induction.The induction rate 96.67% was the highest with 2,4-D 2.0 mg/L+KT 0.1 mg/L for internode.The MS medium with agar 5 g/L was suitable for induction of callus.Condition of the temperature 25℃，the photoperiod of 24 h/d and the light intensity of 6 200 lx was beneficial for callus induction.
参考文献/References:
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备注/Memo
第一作者简介：李利霞（1983-），女，硕士，助理研究员，现主要从事药用植物资源利用与种子种苗繁育和悬浮细胞培养等研究工作。E-mail：silktreedream@126.com. 基金项目：贵州省中药现代化科技产业研究开发专项资助项目（ZY字[2012]3005号）；贵阳市科技计划资助项目（2012HK-209-37）。