[1]焦迎春,余梅,邱冬梅.“草原一号”液体菌种培养基的响应面优化[J].北方园艺,2016,40(02):139-142.[doi:10.11937/bfyy.201602036]
　JIAO Yingchun,YU Mei,QIU Dongmei.Optimizing of the Response Surface Composite Experiment in the Culture Medium of the Liquid of ‘Prairie Strains No.1’[J].Northern Horticulture,2016,40(02):139-142.[doi:10.11937/bfyy.201602036]
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“草原一号”液体菌种培养基的响应面优化

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第40卷 期数: 2016年02 页码: 139-142 栏目: 出版日期: 2016-01-30
Title:
Optimizing of the Response Surface Composite Experiment in the Culture Medium of the Liquid of ‘Prairie Strains No.1’
作者:
焦迎春1; 余梅2; 邱冬梅1
（1.青海大学 农牧学院，青海 西宁 810016；2.青海普兰特药业有限公司，青海 西宁 810007）
Author(s):
JIAO Yingchun1; YU Mei2; QIU Dongmei1
（1.Department of Biological Sciences，Qinghai University，Xining，Qinghai 810016；2.Qinghai Plateau Pharmaceutial Co.Ltd.，Xining，Qinghai 810007）
关键词:
“草原一号”; 液体菌种; 培养基; 响应面; 生物量
Keywords:
Agaricus bisporus (Lange) Imbach.sp; liquid strain; culture medium; the response surface composite; biomass
DOI:
10.11937/bfyy.201602036
文献标志码:
A
摘要:
以1株野生菌株“草原一号”为研究对象，采用单因素试验和响应面试验法，研究培养基中不同营养因子对菌丝体生物量的影响。结果表明：甘露醇29.47 g/L，蛋白胨7.39 g/L，复合维生素B1 1.02 g/L，硫酸镁为0.6 g/L，菌丝体生物量最高可达到25.023 g/L，与试验模型预测值一致。
Abstract:
Taking ‘Prairie Strains No.1’ as the research object，using the single factor test method and combining with the response surface composite experiment method，different nutritional factors impact on the mycelium biomass was investigated.The results showed that grassland best fermentation culture medium for the No.1：mannitol was 29.47 g/L，peptone was 7.39 g/L，vitamin B1 complex was 1.02 g/L，magnesium sulfate was 0.6 g/L，mycelium biomass was the highest,could reach 25.023 g/L，and the response surface prediction were basically identical.

参考文献/References:

 

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备注/Memo

第一作者简介：焦迎春(1973-)，女，河北景县人，硕士，教授,研究方向为食用菌资源的开发利用。E-mail:jyc_22@163.com. 基金项目：青海省科技厅1020资助项目（2013-N-506）；国家自然基金资助项目（31460391）。

更新日期/Last Update: 2016-04-07