DING Yao,LI Jinrong,JIANG Lijian,et al.Effect of Several Factors on Proliferation and Growth of Prunus avium ‘Mingzhu’ in vitro[J].Northern Horticulture,2015,39(22):103-105.[doi:10.11937/bfyy.201522027]
几种因素对大樱桃“明珠”组培苗增殖生长的影响
- Title:
- Effect of Several Factors on Proliferation and Growth of Prunus avium ‘Mingzhu’ in vitro
- Keywords:
- Prunus avium; tissue culture; proliferation; bioreactor
- 文献标志码:
- A
- 摘要:
- 以大连栽植的大樱桃品种“明珠”茎段为外植体，采用组织培养方式，研究了BA、BA与生长素组合、培养瓶透气滤膜有无、不同培养方法对“明珠”组培苗增殖生长的影响。结果表明：在BA 0.5 mg/L+IBA 0.1 mg/L处理中，大樱桃组培苗增殖生长效果最好，分化数量最多，为9.0个/株；在培养瓶盖无透气滤膜的培养瓶中，大樱桃组培苗生长健壮，丛生苗数最多，为6.4个/株；接触式生物反应器培养方法最适合大樱桃的增殖生长，丛生苗数量可达到8.2个/株。
- Abstract:
- The effect of BA，BA/auxin，ventilated filter and culture methods on proliferation and growth of ‘Mingzhu’ by the stems of Prunus avium ‘Mingzhu’ as explants for tissue culture were studied.The results showed that 0.5 mg/L BA and 0.1 mg/L IBA were combined in the medium，the proliferation effect was the best，and the differentiation number reached 9.0.‘Mingzhu’ seedlings grew strongly in the bottle without ventilating filter membrane with 6.4 cluster seedlings per plant.The contact bioreactor culture method was the most suitable for the growth of the proliferation of ‘Mingzhu’，the number of clusters could reach 8.2 per plants.
参考文献/References:
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备注/Memo
第一作者简介：丁遥(1989-)，女，硕士研究生，研究方向为植物组织培养。E-mail：lianmeilan2001@163.com.责任作者：朴炫春(1963-)，男，博士，教授，现主要从事植物组织培养及生物反应器应用等研究工作。E-mail：nyypxc@ybu.edu.cn.基金项目：延边大学大学生创新创业训练计划资助项目（ydbksky2015389）。