LI Haili,TAN Feili,LIU Kaidong,et al.Tissue Culture and Plantlet Regeneration of Ipomoea pes-caprae[J].Northern Horticulture,2015,39(15):104-106.[doi:10.11937/bfyy.201515027]
厚藤的组织培养及植株再生
- Title:
- Tissue Culture and Plantlet Regeneration of Ipomoea pes-caprae
- Keywords:
- Ipomoea pes-caprae; tissue culture; plantlet regeneration
- 文献标志码:
- A
- 摘要:
- 以厚藤不同外植体为试材,MS为基本培养基进行组织培养,研究6-BA和NAA不同浓度组合对不同外植体愈伤组织诱导、不定芽分化及增殖的影响,以筛选出适合厚藤组织培养的最适外植体和培养基。结果表明:厚藤最佳的愈伤组织诱导培养基为MS+6-BA 0.75 mg/L+NAA 0.25 mg/L,最佳外植体为叶片;不定芽诱导培养基为MS+6-BA 1.0 mg/L+NAA 0.1~0.5 mg/L;在MS+6-BA 1.0 mg/L+NAA 0.3 mg/L培养基中增殖倍数最高。在1/2MS培养基上培养,生根率达87.5%。
- Abstract:
- Taking different explants of Ipomoea pes-caprae as material,using MS media with different combination of 6-BA,NAA concentration,the effect of 6-BA,NAA on callus induction,bud initiation,shoot proliferation were studied to optimize the tissue culture of Ipomoea pes-caprae. The results showed that the initial medium for the callus induction was MS+6-BA 0.75 mg/L+NAA 0.25 mg/L,and the optimal explants were leaves.The best medium for adventitious bud differentiation was MS+6-BA 1.0 mg/L+NAA 0.1-0.5 mg/L.The best subculture media for shoot proliferation was MS+6-BA 1.0 mg/L+NAA 0.3 mg/L.The rooting rate was 87.5% in 1/2MS medium.
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备注/Memo
第一作者简介:黎海利(1981-),女,广西宜州人,博士,讲师,研究方向为观赏园艺。E-mail:lihaili2425@126.com. 责任作者:刘锴栋(1982-),男,博士研究生,副研究员,研究方向为园艺学。E-mail:liukaidong2001@126.com. 基金项目:国家星火计划资助项目(2013GA780093);湛江市热带特色资源植物技术开发重点实验室资助项目(2014A06008);岭南师范学院博士启动资助项目(ZL09011)。