TANG Ying-hong,SHEN Fan,LIU Fang,et al.Study on Tissue Culture of Rhododendron fortunei in Xinning[J].Northern Horticulture,2015,39(07):94-97.[doi:10.11937/bfyy.201507028]
新宁云锦杜鹃组织培养研究
- Title:
- Study on Tissue Culture of Rhododendron fortunei in Xinning
- Keywords:
- Rhododendron fortunei; tissue culture; culture medium
- 文献标志码:
- A
- 摘要:
- 以新宁云锦杜鹃茎段为外植体，利用植物组织培养技术，采用不同的外植体消毒方案，以1/4MS为基本培养基，添加了不同浓度的6-BA、ZT和NAA等植物生长调节物质，研究了不同培养条件对云锦杜鹃离体芽诱导、增殖、生根的影响。结果表明：最佳外植体消毒方案为75%乙醇30 s+5%次氯酸钠10 min，转瓶6次去除内生菌，芽诱导的最佳培养基配方是1/4MS+7 mg/L ZT+0.15 mg/L NAA，离体芽在1/4MS+1.0 mg/L ZT+0.10 mg/L NAA培养基上的增殖效果较好，幼苗在1/2MS+0.1 mg/L 6-BA+1.5 mg/L NAA培养基上的生根效果较好。
- Abstract:
- Taking leaves and stems of Rhododendron fortunei as explants，using plant tissue culture techniques，different explant disinfection programs were conducted and using 1/4MS as the basic medium supplemented with different concentrations of ZT and NAA plant growth regulating substances，in order to study the effect of different culture conditions on Rhododendron in vitro bud induction and proliferation.The results showed that the best explant disinfection program was 75% ethanol for 30 seconds and 5% sodium hypochlorite for 10 minutes，and the shoot apex had the highest induced rate.The best bud induction medium program was 1/4MS mixed with 7 mg/L ZT and 0.15 mg/L NAA，in vitro shoots had the best proliferation effect in medium with 1/4MS mixed with 1.0 mg/L ZT and 0.10 mg/L NAA,seedling had the best rooting effect in medium with 1/2MS+0.01 mg/L 6-BA+1.5 mg/L NAA.
参考文献/References:
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备注/Memo
第一作者简介：唐映红（1988-），女，湖南桑植人，硕士研究生，研究方向为作物遗传育种改良和植物组织培养。E-mail：462994081@qq.com. 责任作者：陈建荣（1973-），女，湖南芷江人，博士，副教授，现主要从事植物资源与应用研究及教学工作。基金项目：长沙市科技计划资助项目（K1205040-31）；湖南省科技计划资助项目（2014CK4018）；长沙学院人才引进资助项目(SF080402)。