[1]周 敏,龙秀琴,刘 燕.锈叶杜鹃愈伤组织诱导和增殖研究[J].北方园艺,2015,39(02):88-90.[doi:10.11937/bfyy.201502025]
　ZHOU Min,LONG Xiu-qin,LIU Yan.Study on the Callus Induction and Multiplication of Rhododendron siderophyllum[J].Northern Horticulture,2015,39(02):88-90.[doi:10.11937/bfyy.201502025]
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锈叶杜鹃愈伤组织诱导和增殖研究

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第39卷 期数: 2015年02 页码: 88-90 栏目: 出版日期: 2015-01-30
Title:
Study on the Callus Induction and Multiplication of Rhododendron siderophyllum
作者:
周 敏1; 龙秀琴2; 刘 燕3
(1.遵义医药高等专科学校,贵州 遵义 563000;2.贵州省山地资源所,贵州 贵阳 550001;3.贵州省生物研究所,贵州 贵阳 550009)
Author(s):
ZHOU Min1; LONG Xiu-qin2; LIU Yan3
(1.Zunyi Medical and Pharmaceutical College,Zunyi,Guizhou 563000；2.Guizhou Research Center of Karst Resource Environment and Development,Guiyang，Guizhou 550001；3.Guizhou Institutes of Biology,Guiyang，Guizhou 550009)
关键词:
锈叶杜鹃; 愈伤组织; 诱导; 增殖
Keywords:
Rhododendron siderophyllum; callus; induction; multiplication
DOI:
10.11937/bfyy.201502025
文献标志码:
A
摘要:
以锈叶杜鹃无菌种子萌发后的胚轴和子叶为试材，对影响其愈伤组织诱导和增殖的几个关键因子进行了研究。结果表明：MS培养基较适宜愈伤组织的诱导和培养；TDZ和NAA浓度对愈伤组织诱导有很大影响，TDZ 0.50 mg/L＋NAA 0.05 mg/L对愈伤组织诱导效果较好，诱导率为77.78%；MS+TDZ 0.10 mg/L+NAA 0.05 mg/L对愈伤组织增殖效果较好，增殖率为96.67%，增殖倍数为4.50；愈伤组织需及时转瓶，转瓶时间较晚容易褐化死亡，转瓶时间以40 d为宜。
Abstract:
Taking cotyledon and hypocotyl of seed of Rhododendron siderophyllum as experiment material，the several key factors on callus induction and multiplication of Rhododendron siderophyllum in tussue culture were studied.The results showed that MS medium was suitable for induction and multiplication of callus.The concentrations of TDZ and NAA had a strong influence on callus induction.The MS medium supplemented with TDZ 0.50 mg/L and NAA 0.05 mg/L had high induction effect，and the induction rate was 77.78%.The MS medium supplemented with TDZ 0.10 mg/L and NAA 0.05 mg/L was suitable for callus multiplication，the multiplication rate could reach 96.67%，the multiplication ratio was 4.50.In order to keep callus multiplication more and more，the callus needed to transfer medium again and again.The ideal time for transferring medium was 40 days，so that it could avoid browning of callus.

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备注/Memo

第一作者简介：周敏(1980-)，女，贵州遵义人，硕士，讲师，现主要从事植物组培及栽培技术等研究工作。E-mail:minzhou211@126.com.责任作者：刘燕(1981-),女，贵州贵阳人，硕士，副研究员，现主要从事花卉开发与繁殖等研究工作。基金项目：贵州省科技厅重大专项基金资助项目（黔科合重大专项字[2011]6001）；贵阳市科技局资助项目（筑科合同[20121027]）。

更新日期/Last Update: 2015-02-24