JIAO Lei,CHAI Meng-ying,SHI Ming-sheng,et al.Study on the Extraction Technology of Total Flavonoids From Hawthorn Leaf in Alkaline Conditions[J].Northern Horticulture,2014,38(08):128-130.
碱性条件下山楂叶总黄酮的提取工艺研究
- Title:
- Study on the Extraction Technology of Total Flavonoids From Hawthorn Leaf in Alkaline Conditions
- 文章编号:
- 1001-0009(2014)08-0128-03
- Keywords:
- hawthorn leaves; flavonoids; alkaline; process optimization
- 分类号:
- S 661.5
- 文献标志码:
- A
- 摘要:
- 以山楂叶为试材，以液料比、pH值、提取温度、提取时间为考察指标，进行单因素试验和正交实验，研究了碱性条件下山楂叶中总黄酮的最佳提取工艺。结果表明：最佳提取工艺条件为液料比25∶1 mL/g、pH 8.5、提取温度为90℃，用60%的乙醇提取3 h，此条件下得到的总黄酮提取率为7.97 mg/g。
- Abstract:
- Taking hawthorn leaf as material,based on the different ratio of liquid to solid、pH、extraction temperature、extraction time，the single factor and orthogonal design experiments were conducted,and the optimal extraction process of total flavonoids from hawthorn leaves was obtained.The results showed that ratio of liqutid to material was 25∶1 mL/g,pH was 8.5,extraction temperature was 90℃ extracting with 60% ethanol in 3 h.Verifing in these conditions，flavonoids extracting reached 7.97 mg/g.
参考文献/References:
[1]侯宽照.中国种子植物科属辞典[M].北京：科学出版社,1958：115. [2]李志霞,吕德国,鲁巍巍,等.山楂叶总黄酮提取的主要影响因素研究[J].北方园艺,2010(5)：24-26. [3]郭永学,李楠,杨美燕,等.山楂属植物黄酮成分及其分析方法研究进展[J]．中成药,2005,27(1):112-115. [4]姚新生.天然药物化学[M].3版.北京：人民卫生出版社,2000. [5]李宁,李楚．银杏叶总黄酮提取工艺的正交设计研究[J].山西农业大学学报,2010,30(1)：74-76. [6]鲁巍巍,吕德国,李志霞,等.山楂叶总黄酮三种提取方法的比较研究[J].辽宁农业科技,2008（5）：9-12. [7]张元富,刘志辉,于行梅,等.山楂叶中黄酮类化合物的提取工艺研究[J].中国药业,2009,18(5)：42-43. [8]周海旭,高晗,曲良苗,等.响应面法优化山楂叶中黄酮提取工艺[J].河南农业大学学报,2011,45(6)：711-715.
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备注/Memo
第一作者简介：焦镭(1973-)，男，河南内黄人，硕士，副教授，现主要从事功能性食品研究与开发等工作。