CAO Yun-ying,HOU Hai-tao,LIU Yu-jie,et al.Primary Study on Tissue Culture and Physiology Character of Oncidium[J].Northern Horticulture,2014,38(21):114-118.
文心兰的组织培养及生理特性的初步研究
- Title:
- Primary Study on Tissue Culture and Physiology Character of Oncidium
- 文章编号:
- 1001-0009（2014）21-0114-05
- Keywords:
- Oncidium; tissue culture; protective enzyme activity; isozyme analysis
- 分类号:
- S 682.31
- 文献标志码:
- A
- 摘要:
- 以文心兰试管组织为材料,对适合原球茎、芽苗增殖、壮苗生根的培养基及其生长过程中部分生理生化指标进行了初步研究。结果表明：原球茎最佳增殖培养基为MS+1.0 mg/L 6-BA+0.4 mg/L NAA+1.0 g/L活性炭，不定芽最佳增殖培养基为MS+2.0 mg/L 6-BA+0.5 mg/L NAA+1.0 g/L活性炭，壮苗最佳培养基为MS+0.5 mg/L 6-BA+1.0 mg/L 2,4-D+1.0 g/L活性炭，最佳生根培养基为1/2 MS+0.5 mg/L NAA+1.0 g/L水解酪蛋白。生理测定结果表明，同一种材料由于转接前的培养基不同或不同的继代次数其超氧化物歧化酶（SOD）活性、过氧化物酶（POD）活性存在差异，分化苗转接前NAA为0.1 mg/L的SOD和POD活性高于NAA〖JP4〗为0.5 mg/L，继代次数增加后，其活性会增强，原球茎转接前6-BA为0.5 mg/L的活性比2.0 mg/L的活性高，同工酶谱也证明了这一点。表明原球茎增殖6-BA 2.0 mg/L好于0.5 mg/L，分化苗的增殖NAA 0.5 mg/L好于0.1 mg/L。
- Abstract:
- Taking Oncidium test-tube tissue as materials,the culture medium which was suitable for protocorm;bud seedling proliferation,making seedlings stronger and promoting the root growth of seedlings were screened;some physiological and biochemical indexes of growth process of plantlet regeneration and rooting were also monitored.The results showed that suitable proliferation culture medium for protocorm-like bodies (PLBs) was MS+1.0 mg/L 6-BA+0.4 mg/L NAA+1.0 g/L activated-charcoal,MS+2.0 mg/L 6-BA+0.5 mg/L NAA+1.0 g/L activated-carbon was suitable for adventitious bud multiplication.MS+0.5 mg/L 6-BA+1.0 mg/L 2,4-D+1.0 g/L activated-carbon was better for raising strong plants.1/2 MS+0.5 mg/L NAA+1.0 g/L casein hydrolysate was optimal for producing root.For the same sample,the activity and isozymes of superoxide dismutase and peroxidase because of different culture medium before transferring or the subculture time.SOD and POD activities of the differentiated seedlings sample which transferred from medium containing 0.1 mg/L NAA were higher than those transferred from 0.5 mg/L NAA.With the subculture times increased,its activities enhanced,the activity of sample from medium containing 0.5 mg/L 6-BA was higher than that from 2.0 mg/L 6-BA before transferring for PLBs,isozyme spectrum also proved this point.It suggested that medium containing 2.0 mg/L 6-BA were better than that containing 0.5 mg/L for PLBs proliferation,medium containing 0.5 mg/L NAA were better than that containing 0.1 mg/L for shoot multiplication.
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备注/Memo
第一作者简介：曹云英（1970-），女，博士，副教授，现主要从事植物生理等研究工作。E-mail:cyy@ntu.edu.cn.
基金项目：江苏省高校自然科学研究面上资助项目（13KJB210005）；南通市科技局农业科技创新与产业化资助项目（HL2012028）；南通大学前期预研资助项目（11ZY011）；南通大学博士启动基金资助项目（12B006）。
收稿日期：2014-05-19