LI Xiao-ling,BIAN Zhen,LU Xu-zhi,et al.Study on Rapid Propagation of Cerasus avium ‘Hongdeng’ by Tissue Culture[J].Northern Horticulture,2014,38(20):85-89.
“红灯”大樱桃的组织培养与快速繁殖
- Title:
- Study on Rapid Propagation of Cerasus avium ‘Hongdeng’ by Tissue Culture
- 文章编号:
- 1001-0009（2014）20-0085-05
- Keywords:
- Cerasus avium ‘Hongdeng’; tissue culture; rapid propagation
- 分类号:
- S 662.5
- 文献标志码:
- A
- 摘要:
- 以经引种驯化后的“红灯”大樱桃(Cerasus avium ‘Hongdeng’)耐寒植株的茎尖、腋芽和带腋芽的幼嫩茎段为外植体，对其组培快繁体系进行了比较系统的研究。结果表明：“红灯”大樱桃茎尖及茎段外植体在MS+6-BA 0.8 mg/L+NAA 0.02 mg/L+3% Sucrose+AC 0.5 g/L+0.7% Agar（pH 5.8）培养基上，芽萌发、生长状况较好，萌发率高达90.0%；其组培苗在MS+6-BA 0.6 mg/L+NAA 0.02 mg/L+GA30.1 mg/L+维生素C 40 mg/L+3% Sucrose+0.7% Agar（pH 5.8）培养基上继代培养25 d左右，继代增殖系数均可达到3.0左右；将其中长势良好的组培苗在 1/2MS+NAA 0.8 mg/L+1.5% Sucrose+0.7% Agar（pH 5.8）培养基上生根壮苗培养后，移栽成活率可达86.7%。
- Abstract:
- Taking stem apex,axillary buds and young stems with axillary buds from the cold-tolerant plants of Cerasus avium ‘Hongdeng’ after acclimatization as explants,the protocol of rapid propagation by tissue culture was groped.The results showed that 90% of adventitious buds germinated on the primary medium contained MS basal salts and vitamins,6-BA 0.8 mg/L,NAA 0.02 mg/L,AC 0.5 g/L and 3% sucrose which was solidified with 0.7% agar(pH 5.8).After the shoots in vitro were subcultured for 25 days and so on the subculture medium contained 6-BA 0.6 mg/L,NAA 0.02 mg/L,GA3 0.6 mg/L,vitamin C 40 mg/L,the propagation coefficient by subculture could be up to 3.0.The shoots were rooted and grew stronger the 1/2 MS medium contained NAA 0.8 mg/L,vitamin C 40 mg/L and 1.5% sucrose,and 86.7% of shoots with strong roots survived after they were transplanted to soil.
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备注/Memo
第一作者简介：李晓玲（1971-），女，吉林舒兰人，博士，副教授，现主要从事植物生物技术与分子生物学等研究工作。E-mail：lixiaoling@mail.ccut.edu.cn.
基金项目：吉林省教育厅资助项目（2012121）。
收稿日期：2014-03-29