ZHAO Na-na,CHEN Wen-qiang,DENG Bai-wan,et al.Study of the Genetic Relationships of Main Lentinula edodes Cultispecies in Qinba Mountainous Area[J].Northern Horticulture,2014,38(16):140-143.
秦巴山区香菇主要栽培种亲缘关系的研究
- Title:
- Study of the Genetic Relationships of Main Lentinula edodes Cultispecies in Qinba Mountainous Area
- 文章编号:
- 1001-0009(2014)16-0140-04
- Keywords:
- Lentinula edodes; strain; morphological characteristics; ITS sequence; kinship.
- 分类号:
- S646
- 文献标志码:
- A
- 摘要:
- 以秦巴山区10株香菇主栽菌株为试材，采用组织培养方法对其菌丝生长速度、菌丝形态特征进行分析，采用CTAB法对其DNA提取、PCR扩增及扩增产物进行测序比对，依据ITS序列分析和构建系统进化树。结果表明：“香菇808”和“香菇507”两菌株具有较高生长优势且亲缘关系较远,可作为原生质体制备、融合及再生的菌株。该研究可为秦巴山区香菇主要栽培种亲缘关系的确定和新品种的培育提供理论依据。
- Abstract:
- Using ten main Lentinula edodes cultivated in Qinba mountainous area as malerals,the growth rate,mycelium morphology of they were analyzed by tissue cultured method.Meanwhile,CTAB method was used to extract genomic DNA from the mycelium,amplifying and sequencing PCR products,and ITS analysis of Lentinula edode and phylogenetic tree were conducted.The results showed that ‘Lentinula edodes 808’ and ‘Lentinula edodes 507’ owned growth advantage and distant relationship,as dominant strains protoplast formation,fusion and regeneration.Our findings established a solid foundation to determine the genetic kinship of cultivated species and the cultivation of new varieties in Qinba mountainous area.
参考文献/References:
［1］卯晓岚.中国蕈菌［M］.北京:科学出版社,2009:38.
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备注/Memo
第一作者简介：赵娜娜（1988-），女，甘肃庆阳人，硕士研究生，现主要从事微生物资源开发利用等研究工作。E-mail:xyzh_2080@yeah.net.