ZHANG Xin-lin,YU Hong-chun,FANG Dan-dan,et al.The Screening of High Virulence Bacillus thuringiensis Strains to Chilo suppressalis (Walker)[J].Northern Horticulture,2014,38(16):112-114.
二化螟高毒力苏云金杆菌菌株的筛选
- Title:
- The Screening of High Virulence Bacillus thuringiensis Strains to Chilo suppressalis (Walker)
- 文章编号:
- 1001-0009(2014)16-0112-03
- Keywords:
- Chilo suppressalis (Walker); Bacillus thuringiensis; strains; bioassay; virulence; screening
- 分类号:
- S435.112+.1
- 文献标志码:
- A
- 摘要:
- 以高毒力苏云金芽孢杆菌(Bacillus thuringiensis,Bt)为供试菌株,以二化螟为试虫,初步研究了30个Bt菌株对1~2龄二化螟幼虫的毒力效果,进而以筛选出的5株较高青力Bt菌株进行2~3龄二化螟幼虫青力试验,以筛选出适合黑龙江省二化螟防治的Bt菌株。结果表明:Bt14菌株在饲喂菌液168 h后,二化螟的校正死亡率可达90%以上,毒力回归方程为y=0.6121x+0.8789,R2=0.9989,LC50=5.4×106芽孢/mL,致死剂量与其它菌株差异显著,表明该菌株对防治二化螟具有很好的应用前景。
- Abstract:
- Taking Chilo suppressalis as material,using high virulence Bt strains,the effect of 30 Bt strains on the virulence test of 1~2 instar ?Chilo suppressalis larvaes were studied,then the effect of 5 high virulence Bt strains that screened out on virulence of 2~3 instar Chilo supprealis larvaes were studied,to screen out suitable high virulence Bt strain to prevention Chilo suppressalis in Heilongjiang province.The results showed that the corrected mortality of Chilo suppressalis was 90% treated by Bt14 bacteria liquid after 168 h.And then its toxicity regression equations were:y=0.6121x+0.8789,R2=0.9989.Its LC50?was 5.4×106 spores/mL,which was significant difference from other Bt stains.The results suggested that Bt14 could have a good prospect for controlling Chilo suppressalis.
参考文献/References:
[1]徐艳博,王孟伦,韩兰芝,等.人工饲料、转Bt水稻及其亲本稻苗饲养下二化螟种群适合度的比较研究[J].应用昆虫学报,2012,49(6):1535-1540.
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JIN Da-yong,YU Yong-wan.Effect of Bacillus thuringiensis CAB109 on Growth and Development of Spodoptera exigua Larvae[J].Northern Horticulture,2013,37(16):122.
[2]杨 庆 仙.苏 云 金 杆 菌 增 效 细 菌 研 究 进 展[J].北方园艺,2008,32(01):0.[doi:10.11937/bfyy.200801020]
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备注/Memo
第一作者简介:张鑫琳(1986-),女,黑龙江人,硕士研究生,研究方向为害虫生物防治。E-mail:dnzhangxinlin@163.com.