LIU Zuo-mei,WANG Lan-ying,SONG Xiang-lan,et al.Study on in vitro Culture and Proliferation of Vaccinium corymbosum[J].Northern Horticulture,2013,37(12):103-105.
“南高丛”蓝莓离体培养及增殖技术的研究
- Title:
- Study on in vitro Culture and Proliferation of Vaccinium corymbosum
- 文章编号:
- 1001-0009（2013）12-0103-03
- Keywords:
- Vaccinium corymbosum; culture in vitro; culture media; phytohormone
- 分类号:
- S 663.9
- 文献标志码:
- A
- 摘要:
- 以1 a生“南高丛”蓝莓茎段为试材，对其进行了外植体消毒、丛生芽诱导和增殖培养研究。结果表明：茎段在75%乙醇湿润30 s，0.1%升汞灭菌5～8 min的条件下较易获得无菌外植体；丛生芽诱导最适宜培养基为改良WPM+ZT 1.0 mg/L，萌动率达95%，芽体饱满，健壮；丛生芽增殖培养最适宜培养基为改良WPM+KT 0.5 mg/L，增殖系数为4.1。
- Abstract:
- Taking 1-year-old stem segment of Vaccinium corymbosum as test materials, explants sterilizing,multiple shoot induction and multiplication culture were studied.The results showed that the optimum sterilizing condition was 70% alcohol for 30 s,0.1% HgCl2 for 30 min,by which more explants with higher proliferation rate were obtained.The adventitious bud induction medium were modified WPM+ZT 1.0 mg/L and the highest induction frequency reached 95%,with plumpness and heftiness of bud.The optimal culture medium of bud was modified WPM+ZT 0.5 mg/L,with the multiplication coefficient of 4.1.
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备注/Memo
第一作者简介：刘作梅（1981-），女，江西赣州人，硕士，工程师，现主要从事植物组织培养与林木培育等研究工作。E-mail：liuzuomei703@126.com.
基金项目：江西省科技厅科技支撑计划资助项目（20112BBF60055）。
收稿日期：2013-01-24