WANG Wen-yuan,WANG Wen-he,ZHOU Wen-qiang,et al.The Callus Induction and Shoot Differentiation of Pedicels in Tissue Culture of Iris ensata L.[J].Northern Horticulture,2012,36(21):92-94.
花菖蒲类鸢尾组培愈伤组织诱导及芽的分化研究
- Title:
- The Callus Induction and Shoot Differentiation of Pedicels in Tissue Culture of Iris ensata L.
- 文章编号:
- 1001-0009（2012）21-0092-03
- Keywords:
- Iris ensata L.; tissue culture; induction; differentiation
- 分类号:
- S 682.2+4
- 文献标志码:
- A
- 摘要:
- 以日本花菖蒲类鸢尾的2个品种为试材，进行了愈伤组织的诱导和不定芽分化的组织培养试验。结果表明：日本花菖蒲类鸢尾组织培养最佳的外植体是花茎，而且是在花苞长度为2~5 cm左右处取样最佳，灭菌以采用0.1% HgCl2消毒10 min效果最好，污染率仅为15%，成活率能达75%。诱导愈伤组织和不定芽分化的最佳培养基为MS+6-BA 1.0 mg/L+NAA 1.0 mg/L，愈伤组织诱导率为59.09%，不定芽的分化率为50%。
- Abstract:
- Two species of Japanese Iris ensata L.were used as test materials to develop callus induction and shoot differentiation of pedicels.The results showed that flower stalks was the best explants when the length of flower bud was 2~5 cm，and disinfected with 0.1% HgCl2 for 10 min were the most effective with the average contamination rate of 15.00% and survival rate of 75%.The optimal medium developing callus induction and shoot differentiation of pedicels was MS medium supplemented with 1.0 mg/L 6-BA and 1.0 mg/L NAA，the induction rate was 59.09% and differentiation rate was 50%.
参考文献/References:
[1]Austin C.IRISES：A gardener’s encyclopedia[M].Timber Press，2005:235-238. [2]赵毓棠.鸢尾欣赏与栽培利用[M].北京:金盾出版社,2005. [3]黄苏珍,郭维明.中国鸢尾属观赏植物资源的研究与利用[J].中国野生植物资源研究,2003,22(1):4-7. [4]陈晨,毕晓颖,卢明艳.德国鸢尾组织培养快速繁殖技术研究[J].沈阳农业大学学报,2010,41(1):27-32. [5]黄苏珍,韩玉林,谢明云,等.德国鸢尾的组织培养[J].江苏林业科技,2000,27(6):37-44. [6]黄苏珍,韩玉林,谢明云,等.杂种鸢尾的组织培养与植株再生[J].植物生理学通讯,2003,36(6):638. [7]吴月燕,毛军平,周倩倩.路易斯鸢尾组织培养过程中愈伤组织的诱导和芽的分化[J].浙江农业科学,2009(1):86-89. [8]徐立军,管耀义,张建佐.有髯鸢尾“爱抚”的组培快繁研究[J].河北林业科技,2005,8(4):79. [9]黄苏珍,韩玉林,谢明云,等.杂种鸢尾的组织培养和植株再生[J].植物生理学通讯,2003,39(6):638. [10]黄淑珍,谢明云,佟海英,等.荷兰鸢尾的组织培养[J].植物资源与环境,1999,8(3):48-52.
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备注/Memo
第一作者简介：王文元（1971-），男，辽宁沈阳人，硕士，高级工程师，现主要从事园林植物引种栽培和繁殖技术的研究工作。E-mail:wwyxyf@yahoo.com.cn.