XU Qi-hong,REN Ping-guo.Study on Cryo-preservation Technology with Vitrification of ‘Tongzi No.1’ Strawberry[J].Northern Horticulture,2012,36(20):117-119.
“童子一号”草莓玻璃化法超低温保存技术研究
- Title:
- Study on Cryo-preservation Technology with Vitrification of ‘Tongzi No.1’ Strawberry
- 文章编号:
- 1001-0009（2012）20-0117-03
- Keywords:
- strawberry; cryopreservation; vitrification
- 分类号:
- S 668.409+.3
- 文献标志码:
- A
- 摘要:
- 以玻璃化法进行“童子一号”草莓离体茎尖的超低温保存研究,探讨了低温锻炼时间、预培养时间、预处理时间、PVS2处理时间等对存活率的影响。结果表明：将4℃条件下低温锻炼30 d的健壮草莓苗茎尖，接种在固体预培养培养基中预培养4 d，预处理30 min后，吸出预处理液，加入PVS2，0℃处理80 min后，更换新鲜的PVS2，并迅速投入液氮60 min后，置于40℃快速化冻1 min；室温洗涤3次并取出茎尖，接种到再生培养基恢复培养；成活率最高可达43.00%，且再生植株分化正常。
- Abstract:
- Researching on the ‘Tongzi No.1’strawberry meristem-tip cryo-preservation with vitrification，the effect of various factors of vitrification，such as，low temperature acclimatization time，pre-culture time，pretreatment time，PVS2 treatment time，were studied on survival rate of strawberry meristem-tip after cryo-preservation.The results showed that after 30 d and acclimatizing strawberry meristem-tips at 4℃，they were cultured in solid preculture medium after 4 d；pretreat for 30 min，suck up the pre-culture medium，and then put them into PVS2 at 0℃ for 80 min；then put them in the fresh PVS2，pre-serve in LN for 1 h；and then throw them rapidly into water at 40℃ for 1 min；wash the shoot tips 3 times at room temperature and then plant the tips to regeneration medium.The survival rate could reach the highest about 43% and rege-nerative plants become divided regularly.
参考文献/References:
[1]朱至清.植物细胞工程[M].北京:化学出版社,2003：34-51. [2]薛庆善.体外培养的原理和技术[M].北京:科学出版社,2001:1173-1179. [3]Nag K K,Street H E.Carrot embryogenesis from frozen cultured cells[J].Nature,1973,245:270-272. [4]王关林,方宏筠.植物基因工程原理与技术[M].北京:科学出版社,1998. [5]王子成,邓秀新.玻璃化法超低温保存柑桔茎尖及植株再生[J].园艺学报，2001,28(4):301-306. [6]吴永杰,赵艳华,周明德.苹果休眠茎尖的超低温保存研究[J].华北农学报，1999,14(1)：129-133. [7]李广武,郑从义,唐兵.低温生物学[M].湖南:科学技术出版社,1998. [8]肖洁凝,黄学林.茎尖和芽的超低温保存[J].生物工程进展,1999,19(5):46-51. [9]赵云,潘涛,王茂林.组织培养技术在植物种质保存中的应用[J].种子,1995(6):58-60. [10]马锋旺,李嘉瑞.杏原生质体的超低温保存[J].园艺学报,1998,25(4):329-332. [11]简令成,孙龙华.猕猴桃茎段的超低温保存[J].植物学报,1989,31(1):66-68. [12]Sakai A.Development of cryopreservation techniques.Florent E,Hiroko T.Cryopreservation of tropical plant germplasm-current research progress and aplication[C].Tsukuba：Japan Internation Research Center for Agriculture Sciences,2000：1-7. [13]王君晖,边红武,黄纯农.植物样品包埋脱水法超低温保存的研究进展[J].植物学通报,1999,16(5):582-585. [14]赵艳华,吴永杰,陈霜莹,等.包埋千燥超低温保存苹果离体茎尖[J].园艺学报,1998,25(1):93-95. [15]吴雪梅,汤浩茹.包埋玻璃化法超低温保存植物种质的研究进展[J].植物学通报，2005（22）:238-245. [16]王君晖,张毅翔,刘峰,等.铁皮石斛种子、原球茎和类原球茎体的超低温保存研究[J].园艺学报,1999,26(1):59-61.
相似文献/References:
[1]杜来顺,冯建军,李晓华,等.生姜玻璃化法超低温保存技术[J].北方园艺,2014,38(13):123.
DU Lai-shun,FENG Jian-jun,LI Xiao-hua,et al.Ginger Vitrification Cryopreservation Technology[J].Northern Horticulture,2014,38(20):123.
[2]杨 爽,李海鹏,杨培鑑,等.微生物菌肥对草莓光合特性的影响[J].北方园艺,2014,38(11):165.
YANG Shuang,LI Hai-peng,YANG Pei-jian,et al.Effect of Biofertilizer on Photosynthesis Characters of Strawberries[J].Northern Horticulture,2014,38(20):165.
[3]杨 爽,李海鹏,杨培鑑,等.不同草莓品种对白粉病的抗性调查[J].北方园艺,2014,38(10):104.
YANG Shuang,LI Hai-peng,YANG Pei-jian,et al.Survey on the Resistance to the Powdery Mildew of Different Strawberries Cultivars[J].Northern Horticulture,2014,38(20):104.
[4]李 邵,田 婧,鲁少尉,等.设施草莓高架分层育苗系统育苗效果研究[J].北方园艺,2014,38(14):41.
LI Shao,TIAN Jing,LU Shao-wei,et al.Study on Nursery Effect of an Elevated and Layered Seedling Raising System for Greenhouse Strawberry[J].Northern Horticulture,2014,38(20):41.
[5]刘万达,张丙秀,魏媛媛,等.草莓DHAR基因密码子偏好性分析[J].北方园艺,2014,38(14):92.
LIU Wan-da,ZHANG Bing-xiu,WEI Yuan-yuan,et al.Analysis of Codon Bias of DHAR Gene in Strawberry[J].Northern Horticulture,2014,38(20):92.
[6]梁新安,梁芳芳.高品质草莓日光温室栽培技术[J].北方园艺,2013,37(13):56.
LIANG Xin-an,LIANG Fang-fang.High Quality Strawberry Cultivation Techniques in Solar Greenhouse[J].Northern Horticulture,2013,37(20):56.
[7]李星月,刘奇志,李贺勤,等.大棚草莓棉蚜分布规律及其生态调控意义研究[J].北方园艺,2014,38(02):124.
LI Xing-yue,LIU Qi-zhi,LI He-qin,et al.Research on Spatial Distribution and Ecological Control of Aphis gossypii Glover in Greenhouse Strawberry[J].Northern Horticulture,2014,38(20):124.
[8]赵菊莲.丛枝菌根真菌诱导草莓枯萎病抗性机理研究[J].北方园艺,2013,37(17):115.
ZHAO Ju-lian.Study on Mechanism of Strawberry Fusarium Wilt Resistance Induced by Arbuscular Mycorrhizae Fungus[J].Northern Horticulture,2013,37(20):115.
[9]万 红,陶 磅,孔令明,等.草莓有机生态型盆栽基质研究[J].北方园艺,2014,38(08):149.
WAN Hong,TAO Pang,KONG Ling-ming,et al.Research on Substrate for Eco-organic Pot Culture of Strawberry[J].Northern Horticulture,2014,38(20):149.
[10]李建龙,王永和,成太祥,等.蚯蚓粪改良剂对草莓产量和品质的影响[J].北方园艺,2014,38(06):163.
LI Jian-long,WANG Yong-he,CHENG Tai-xiang,et al.Effect of Earthworm-cast Modifier on the Yield and Quality of Strawberry[J].Northern Horticulture,2014,38(20):163.
备注/Memo
第一作者简介：徐启红（1973-），女，河南沈丘人，硕士，副教授，现主要从事植物生理生化的教学与研究工作。E-mail:xqhryf@126.com.