XU Qi-hong,REN Ping-guo.Study on Cryo-preservation Technology with Vitrification of ‘Tongzi No.1’ Strawberry[J].Northern Horticulture,2012,36(20):117-119.
“童子一号”草莓玻璃化法超低温保存技术研究
- Title:
- Study on Cryo-preservation Technology with Vitrification of ‘Tongzi No.1’ Strawberry
- 文章编号:
- 1001-0009（2012）20-0117-03
- Keywords:
- strawberry; cryopreservation; vitrification
- 分类号:
- S 668.409+.3
- 文献标志码:
- A
- 摘要:
- 以玻璃化法进行“童子一号”草莓离体茎尖的超低温保存研究,探讨了低温锻炼时间、预培养时间、预处理时间、PVS2处理时间等对存活率的影响。结果表明：将4℃条件下低温锻炼30 d的健壮草莓苗茎尖，接种在固体预培养培养基中预培养4 d，预处理30 min后，吸出预处理液，加入PVS2，0℃处理80 min后，更换新鲜的PVS2，并迅速投入液氮60 min后，置于40℃快速化冻1 min；室温洗涤3次并取出茎尖，接种到再生培养基恢复培养；成活率最高可达43.00%，且再生植株分化正常。
- Abstract:
- Researching on the ‘Tongzi No.1’strawberry meristem-tip cryo-preservation with vitrification，the effect of various factors of vitrification，such as，low temperature acclimatization time，pre-culture time，pretreatment time，PVS2 treatment time，were studied on survival rate of strawberry meristem-tip after cryo-preservation.The results showed that after 30 d and acclimatizing strawberry meristem-tips at 4℃，they were cultured in solid preculture medium after 4 d；pretreat for 30 min，suck up the pre-culture medium，and then put them into PVS2 at 0℃ for 80 min；then put them in the fresh PVS2，pre-serve in LN for 1 h；and then throw them rapidly into water at 40℃ for 1 min；wash the shoot tips 3 times at room temperature and then plant the tips to regeneration medium.The survival rate could reach the highest about 43% and rege-nerative plants become divided regularly.
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备注/Memo
第一作者简介：徐启红（1973-），女，河南沈丘人，硕士，副教授，现主要从事植物生理生化的教学与研究工作。E-mail:xqhryf@126.com.