REN Zhen.Research on Tissue Culture of Chosenia arbutifolia(Pall) A.Skv.[J].Northern Horticulture,2012,36(18):144-146.
钻天柳组织培养技术研究
- Title:
- Research on Tissue Culture of Chosenia arbutifolia(Pall) A.Skv.
- 文章编号:
- 1001-0009（2012）18-0144-03
- 分类号:
- S 792.12
- 文献标志码:
- B
- 摘要:
- 以钻天柳的休眠枝茎段为外植体，研究了杀菌时间、植物激素、栽培基质对其试管苗生根及移栽后成活率的影响。结果表明：以0.1%HgCl2表面灭菌8 min效果最好；在继代增殖培养阶段，MS+BA 1.00 mg/L+NAA 0.03 mg/L丛生芽诱导率和增殖率最高，分别为96.3%和（28.2±2.8）%。在1/2MS+ IBA 0.5 mg/L培养基中的生根效果最好，生根率达到98.5%，移栽时，选择蛭石+草炭土（体积比为1∶1）较好，且有利于试管苗的成活及生长。
- Abstract:
- Taking the dormancy stem section as explant,the effect of sterilization time,plant hormone and cultivation on rooting and trasplanting of Chosenia arbutifolia(Pall) A.Skv. were studied.The results showed that sterilization time was best with 0.1%HgCl2 in 8 min,in the subculture stage，shoots induction rate and multiplication rate were the highest，96.3% and (28.2±2.8)%，respectively on the medium MS+BA 1.00 mg/L+NAA 0.03 mg/L.The shoots roots well on the basic medium 1/2MS+IBA 0.5 mg/L and the rooting rate was 98.5%.In domestication，it was better to use vermiculite and leaf mold for the growth of tender shoots in the transplanting soils.
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备注/Memo
作者简介：任真(1978-)，女，辽宁人，本科，工程师，现主要从事园林树木花卉栽培工作。E-mail：renzhen1978@163.com.