REN Zhen.Research on Tissue Culture of Chosenia arbutifolia(Pall) A.Skv.[J].Northern Horticulture,2012,36(18):144-146.
钻天柳组织培养技术研究
- Title:
- Research on Tissue Culture of Chosenia arbutifolia(Pall) A.Skv.
- 文章编号:
- 1001-0009（2012）18-0144-03
- 分类号:
- S 792.12
- 文献标志码:
- B
- 摘要:
- 以钻天柳的休眠枝茎段为外植体，研究了杀菌时间、植物激素、栽培基质对其试管苗生根及移栽后成活率的影响。结果表明：以0.1%HgCl2表面灭菌8 min效果最好；在继代增殖培养阶段，MS+BA 1.00 mg/L+NAA 0.03 mg/L丛生芽诱导率和增殖率最高，分别为96.3%和（28.2±2.8）%。在1/2MS+ IBA 0.5 mg/L培养基中的生根效果最好，生根率达到98.5%，移栽时，选择蛭石+草炭土（体积比为1∶1）较好，且有利于试管苗的成活及生长。
- Abstract:
- Taking the dormancy stem section as explant,the effect of sterilization time,plant hormone and cultivation on rooting and trasplanting of Chosenia arbutifolia(Pall) A.Skv. were studied.The results showed that sterilization time was best with 0.1%HgCl2 in 8 min,in the subculture stage，shoots induction rate and multiplication rate were the highest，96.3% and (28.2±2.8)%，respectively on the medium MS+BA 1.00 mg/L+NAA 0.03 mg/L.The shoots roots well on the basic medium 1/2MS+IBA 0.5 mg/L and the rooting rate was 98.5%.In domestication，it was better to use vermiculite and leaf mold for the growth of tender shoots in the transplanting soils.
参考文献/References:
[1]
王向军
,
陶晶
,
张士俊
,
等
.
钻天柳腋芽组培快繁技术的研究
[J].
吉林林业科技
,2006,35(3)
：
8-11.
[2]闫学民,张凤杰,付玉杰,等.钻天柳繁育技术研究[J].辽宁林业科技,2001(1):3-5,28. [3]顾建新,李芳艳,王蓉,等.草莓组培工厂化育苗大规模生产技术[J].新疆农业科学,2008,45(A01):131-133. [4]陈兴华,何旭君,张华通,等.也门铁组培工厂化育苗技术路线[J].仲恺农业工程学院学报,2010,23(2):21-24. [5]高秀花,郭秀芬.大樱桃脱毒苗组培工厂化育苗技术[J].河北果树,2006(5):27-28. [6]顾地周,高捍东,顾美影,等.应用均匀设计法优化钻天柳嫩叶柄植株再生体系[J].东北林业大学学报,2009,37(11):21-23.
相似文献/References:
[1]王海新,赵艳,尤淑丽,等.植物生长调节剂“易丰收”对树莓组培苗生根的影响[J].北方园艺,2013,37(04):31.
[2]陶米林,刘清波,黄红梅,等.半夏组织培养体系的建立[J].北方园艺,2013,37(04):113.
[3]曹 虹,金荣荣.三倍体薄皮甜瓜未成熟胚子叶诱导不定芽的研究[J].北方园艺,2014,38(13):106.
CAO Hong,JIN Rong-rong.Study on Adventitious Bud From Immature Embryo Cotyledon of Triploid Muskmelon[J].Northern Horticulture,2014,38(18):106.
[4]张振霞,李彩萍,张秋枚,等.甘肃贝母的组织培养和植株再生研究[J].北方园艺,2014,38(10):80.
ZHANG Zhen-xia,LI Cai-ping,ZHANG Qiu-mei,et al.Study on Tissue Culture and Plant Regeneration of Fritillaria przewalskii Maxim[J].Northern Horticulture,2014,38(18):80.
[5]王玉娇,杨 忠,赵春莉,等.兴安白头翁组织培养与快繁技术研究[J].北方园艺,2014,38(10):84.
WANG Yu-jiao,YANG Zhong,ZHAO Chun-li,et al.Study on Tissue Culture and Rapid Propagation of Pulsatill dahurica（Fisch.）Spreng[J].Northern Horticulture,2014,38(18):84.
[6]朱 军,李晓瑾,孙 丽,等.药用植物玛咖离体快繁技术研究[J].北方园艺,2013,37(22):101.
ZHU Jun,LI Xiao-jin,SUN Li,et al.Study on the Rapid Propagation of Medicinal Plants Lepidium meyenii[J].Northern Horticulture,2013,37(18):101.
[7]杜 丽.香樟子叶胚培养的研究[J].北方园艺,2014,38(14):89.
DU Li.Research on Cotyledonary Embryo Culture of Cinnamomum camphora L.[J].Northern Horticulture,2014,38(18):89.
[8]赵丽莉,徐芳芳,荀 洋,等.桔梗花药愈伤组织诱导培养基筛选[J].北方园艺,2014,38(01):95.
ZHAO Li-li,XU Fang-fang,XUN Yang,et al.Screening of Callus Induction Medium of Platycodon grandiflorumAnther[J].Northern Horticulture,2014,38(18):95.
[9]李云玲,孙 虎,苗锦山,等.黄山贡菊茎尖脱毒快繁技术研究[J].北方园艺,2014,38(01):106.
LI Yun-ling,SUN Hu,MIAO Jin-shan,et al.Tissue Culture and Rapid Propagation of Chrysanthemum morifolium[J].Northern Horticulture,2014,38(18):106.
[10]陈丽文,杨利平,荣薏,等.金心也门铁工厂化育苗技术研究[J].北方园艺,2013,37(13):138.
CHEN Liwen,YANG Li-ping,RONG Yi,et al.Study on the Techniques of Industrialized Seedling Culture of Draceana arborea[J].Northern Horticulture,2013,37(18):138.
备注/Memo
作者简介：任真(1978-)，女，辽宁人，本科，工程师，现主要从事园林树木花卉栽培工作。E-mail：renzhen1978@163.com.