LIU Yan-ji,HAO Gui-jie,JIANG Yan-yan,et al.Study on the Optimal Method of Tissue Culture on Different Species of Wild Actinidia arguta[J].Northern Horticulture,2012,36(17):124-126.
不同品种野生软枣猕猴桃最佳组培方法的研究
- Title:
- Study on the Optimal Method of Tissue Culture on Different Species of Wild Actinidia arguta
- 文章编号:
- 1001-0009(2012)17-0124-03
- 分类号:
- S 663.4
- 文献标志码:
- B
- 摘要:
- 以5个品种野生软枣猕猴桃（H1、H2、H3、H4和H5）茎段为外植体，采用6-BA和NAA的组合进行研究,筛选适宜于野软枣猕猴桃各品种快繁的最佳培养基，以期建立离体培养快繁体系。结果表明：品种和培养基对猕猴桃外植体扩繁有显著影响；H1在MS+6-BA 1.0 mg/L+NAA 0.2 mg/L的培养基中最适宜扩繁；H2 在MS+6-BA 1.5 mg/L +NAA 0.2 mg/L的培养基中最适宜扩繁；H3和H4在培养基MS+6-BA 1.5 mg/L +NAA 0.3 mg/L中最适宜扩繁；H5 在的培养基MS+6-BA 2.0 mg/L +NAA 0.2 mg/L中最适宜扩繁。
- Abstract:
- The tissue culture system about different species of wild Actinidia arguta (H1、H2、H3、H4 and H5) were established by using their stem segments explants.The best medium was studied and screened，which suited for different kinds of wild Actinidia arguta rapid propagation by the combination of 6-BA and NAA.The results indicated that the species and mediums had significantly impact on the stem segments explants.The most suitable medium for propagation in species H1 was MS+6-BA 1.0 mg/L+NAA 0.2 mg/L，the most suitable medium for propagation in species H2 was MS+6-BA 1.5 mg/L+NAA 0.2 mg/L；the most suitable medium for propagation in species H3 and H4 was MS+6-BA 1.5 mg/L+NAA 0.3 mg/L,the most suitable medium for propagation in species H5 was MS+6-BA 2.0 mg/L+NAA 0.2 mg/L.
参考文献/References:
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备注/Memo
第一作者简介：刘延吉（1959-），男，博士，副教授，研究方向为植物次生物质与功能食品。E-mail：yanjiliu@yahoo.com.cn.