WANG Ping-ping,WANG Yu-jiao,CHEN Xu-hui,et al.Study on the Affecting Factors of Growth of Oreorchis patens Mycorrhizae in vitro[J].Northern Horticulture,2012,36(09):66-69.
山兰菌根真菌离体培养条件的研究
- Title:
- Study on the Affecting Factors of Growth of Oreorchis patens Mycorrhizae in vitro
- 文章编号:
- 1001-0009（2012）09-0066-04
- Keywords:
- Oreorchis patens; mycorrhizae; culture conditions; growth rate
- 分类号:
- S 682.31
- 文献标志码:
- A
- 摘要:
- 通过分离、纯化、培养山兰菌根菌，设置温度、光照、碳源、氮源和pH值等培养条件进行单因素试验，研究山兰菌根菌在不同培养条件下生长状况及培养性状，并对数据进行方差分析。结果表明：该山兰菌根菌在5～30℃都能生长，最适温度是20℃；在各种光照条件下均能生长，其中12 h光照、12 h黑暗条件下山兰菌根菌生长最好；适宜生长的pH 5.0～6.0；在碳源方面对葡萄糖利用最好；在氮源方面对甘氨酸利用最好。
- Abstract:
- By means of separating，purifying，cultivating mycorrhizae of Oreorchis patens，setting temperature，light condition，carbon sources，nitrogen source，acidity and alkalinity and so on for single-factor test，the growth conditions and culture traits of mycorrhizae of Oreorchis patens were studied in different culture conditions.The results showed that Oreorchis patens mycorrhizae could grow within 5～30℃，and 20℃ was its optimum temperature; In all kinds of light conditions could grow，the optimum condition was 12 hours of light and 12 hours of night; the optimum pH was pH 5.0 to 7.0; its optimal carbon sources was glucose; the optimal was glycine.
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备注/Memo
第一作者简介：王平平(1987-)，女，在读硕士，现主要从事濒危植物保护方面的研究工作。
责任作者：曲波(1972-)，女，博士，副教授，现主要从事植物资源保护研究工作。E-mail:qubo-@163.com。
基金项目：国家自然科学基金资助项目（31100299）；高等学校博士学科点专项科研基金资助项目（20112103120016）；辽宁省教育厅科学研究一般资助项目（L2011140）。
收稿日期：2012-02-22