[1]田凡,朱国胜,桂阳,等.硬叶兜兰菌根真菌的分离及培养特性研究[J].北方园艺,2012,36(07):61-64.
　TIAN Fan,ZHU Guo-sheng,GUI Yang,et al.Study on Isolation and Culture Characteristics of Paphiopedilum micranthum Mycorrhizal Fungi[J].Northern Horticulture,2012,36(07):61-64.

点击复制

硬叶兜兰菌根真菌的分离及培养特性研究

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第36卷期数: 2012年07期页码: 61-64 栏目: 出版日期: 2012-07-16

Title:: Study on Isolation and Culture Characteristics of Paphiopedilum micranthum Mycorrhizal Fungi

文章编号:: 1001-0009（2012）07-0061-04

作者:: 田凡¹; 朱国胜²; 3; 桂阳²; 3; 白新祥¹; 1.贵州大学，贵州贵阳 550025；
2.贵州省农业科学院，贵州省现代中药材研究所，贵州贵阳 550006；
3.贵州省农业生物技术重点实验室，贵州贵阳 550006

Author(s):: TIAN Fan¹; ZHU Guo-sheng ²; 3; GUI Yang ²; 3; BAI Xin-xiang¹; 1.Guizhou University，Guiyang，Guizhou 550025;
2.Institute of Morden Chinese Medical Materials，Guizhou Academy of Agricaltural Sciences，Guiyang，Guizhou 550006；
3.Guizhou Key Laboratory of Agricultural Biotechnology，Guiyang，Guizhou 550006

关键词:: 硬叶兜兰; 菌根真菌; 菌丝团; 分离; 培养

Keywords:: Paphiopedilum micranthum; mycorrhizal fungi; peloton; isolation; culture

分类号:: S 682.31

文献标志码:: A

摘要:: 采用单菌丝团法分离硬叶兜兰菌根真菌，纯化后，用打孔法培养菌株并观察形态特征并测定生长速度，初步鉴定了8株内生真菌（DJYYDL001、DJYYDL003、DJYYDL004、DJYYDL006、DJYYDL008、DJYYDL009、DJYYDL011、DJYYDL015）。结果表明：单菌丝团法分离出的菌丝团在恒温培养12 h后镜检及吸取萌发菌丝团为最佳时间。没有经过升汞处理的根段菌丝团污染率最大，而处理2~3 min后根段的菌丝团污染率最小。

Abstract:: Eight mycorrhizal endophyte strains were isolated from a single peloton of Paphiopedilum micranthum.After purification，the strains were cultured by the hole method and the morphological characteristics were observed.At the same time，the growth rate was determinated.Isolated strains were named as DJYYDL001,DJYYDL003,DJYYDL004，DJYYDL006，DJYYDL008,DJYYDL009,DJYYDL011,DJYYDL015.The results showed that the single sclerotium isolated from the pelotons were cultured in thermostatic,after 12 hours，microscopy and absorb the hyphae grow group was the best time.Without mercuric chloride treatment root sclerotium pollution rate，while processing 2~3 min posterior root segment sclerotium contamination rate minimum.

参考文献/References:

[1]陈心启,吉占和.中国兰花全书[M].北京:中国林业出版社,1997:255-256.
[2]刘仲健,陈心启,陈利君,等.中国兜兰属植物[M].北京:科学出版社,2009:41-47.
[3]龙波,龙春林.兜兰属植物及其研究现状[J].自然杂志,2006,28(6):341-344.
[4]李明,张灼.杏黄兜兰菌根研究与应用[J].生物学杂志,2001,18(6):17-18.
[5]罗玉容.硬叶兜兰菌根真菌的观察、分离和鉴定[D].广州:华南农业大学,2009.
[6]Li Y，Yang Z L，Li S Y，et al.Mycorrhizal specificity,preference,and plasticity of six slipper orchids from South Western China[J].Mycorrhiza,2010,20:559-568.
[7]Zhu G S,Yu Z N,Gui Y,et al.A novel technique for isolating orchid mycorrhizal fungi[J].Fungal Diversity,2008,33:123-137.
[8]朱国胜.贵州特色药用兰科植物杜鹃兰和独蒜兰共生真菌研究与应用[D].武汉：华中农业大学,2009.
[9]黄永会,朱国胜,刘作易,等.杜鹃兰菌根结构显微观察初报[J].贵州农业科学,2007,35(1):16-17.
[10]罗毅波,贾建生,王春玲.中国兰科植物保育的现状和展望[J].生物多样性,2003,11(1):70-77.

相似文献/References:

[1]赵菊莲.丛枝菌根真菌诱导草莓枯萎病抗性机理研究[J].北方园艺,2013,37(17):115.
　ZHAO Ju-lian.Study on Mechanism of Strawberry Fusarium Wilt Resistance Induced by Arbuscular Mycorrhizae Fungus[J].Northern Horticulture,2013,37(07):115.
[2]龚娜,肖军,杨镇,等.不同菌根真菌对蓝莓根际土壤微生物数量的影响[J].北方园艺,2013,37(17):175.
　GONG Na,XIAO Jun,YANG Zhen,et al.Effects of Different Mycorrhizal Fungi on Soil Microbial Number in the Blueberry Rhizosphere[J].Northern Horticulture,2013,37(07):175.
[3]张艺子涵,张群,王平平,等.羊耳蒜菌根真菌培养条件的优化[J].北方园艺,2014,38(07):102.
　ZHANG Yi-zihan,ZHANG Qun,WANG Ping-ping,et al.Optimization of Culture Conditions on the Liparis Mycorrhizal Fungi[J].Northern Horticulture,2014,38(07):102.
[4]王平平,王玉娇,陈旭辉,等.山兰菌根真菌离体培养条件的研究[J].北方园艺,2012,36(09):66.
　WANG Ping-ping,WANG Yu-jiao,CHEN Xu-hui,et al.Study on the Affecting Factors of Growth of Oreorchis patens Mycorrhizae in vitro[J].Northern Horticulture,2012,36(07):66.
[5]王平平,陈旭辉,曲波,等.不同培养条件对绶草菌根真菌生长的影响[J].北方园艺,2012,36(19):170.
　WANG Ping-ping,CHEN Xu-hui,QU Bo,et al.Effect of the Different Culture Conditions on the Growth of Spiranthes sinensis Mycorrhizae[J].Northern Horticulture,2012,36(07):170.
[6]段雪甜,陈雨薇,王芳,等.连瓣兰组织培养及菌根化研究[J].北方园艺,2015,39(15):111.[doi:10.11937/bfyy.201515029]
　DUAN Xuetian,CHEN Yuwei,WANG Fang,et al.Study on Tissue Culture and Mycorrhizal Fungi of Cymbidium lianpan[J].Northern Horticulture,2015,39(07):111.[doi:10.11937/bfyy.201515029]
[7]殷小琳,等.菌根对植物抗盐碱性的影响机理研究[J].北方园艺,2010,34(05):0.[doi:10.11937/bfyy.201005085]
　YIN Xiao -lin,W ANG Dong-mei,DING Guo -dong,et al.Research on Mycorrhizal Fungi on Resist of saline -alkali[J].Northern Horticulture,2010,34(07):0.[doi:10.11937/bfyy.201005085]
[8]丁长春,李　蕾,夏念和.硬叶兜兰的无菌播种和试管成苗[J].北方园艺,2011,35(05):0.[doi:10.11937/bfyy.201105043]
　DING Chang -chun,LI Lei,et al.Aseptic Sowing and invitro Seedling Culture of Paphiopedilummicranthum T. T ang &F. T. Wang[J].Northern Horticulture,2011,35(07):0.[doi:10.11937/bfyy.201105043]
[9]李　焱.菌根真菌和腐植酸有机肥对樱桃番茄产量和品质的影响[J].北方园艺,2009,33(08):0.[doi:10.11937/bfyy.200908029]
　LI Yan.Effects of Arbuscular Mycorrhizal Fungi on Yield and Quality of Cherry T omato under Humic Acid[J].Northern Horticulture,2009,33(07):0.[doi:10.11937/bfyy.200908029]
[10]周玉杰,杨福孙,宋希强,等.菌根真菌对华石斛幼苗生长及光合性能的影响[J].北方园艺,2009,33(12):0.[doi:10.11937/bfyy.2009012003]
　ZHOU Yu -jie,YANG Fu -sun,SONG Xi -qiang,et al.Effects of Mycorrhizal Fungi on Seedling’ s Growth and Photosynthetic Capability of Dendrobiumsinense ,Endemic to Hainan[J].Northern Horticulture,2009,33(07):0.[doi:10.11937/bfyy.2009012003]
[11]田凡,廖小锋,王莲辉,等.硬叶兜兰菌根真菌的分类鉴定[J].北方园艺,2017,41(24):116.[doi:10.11937/bfyy.20171417]
　TIAN Fan,LIAO Xiaofeng,WANG Lianhui,et al.Classification and Identifition of Mycorrhizal Fungi of Paphiopedilum micranthum[J].Northern Horticulture,2017,41(07):116.[doi:10.11937/bfyy.20171417]

备注/Memo

第一作者简介：田凡(1985-)，女，硕士，现主要从事兰科植物菌根真菌研究工作。
责任作者：白新祥(1979-)，男，博士，副教授，现主要从事兰科花卉资源的遗传多样性与保护利用研究工作。E-mail：254715174@qq.com。
基金项目：贵州省科技厅社会发展科技攻关资助项目（黔科合SY字[2008]3020号）；贵州大学人才引进基金资助项目（贵大人基合字[2007]41号）；贵州省农业科学院博士科研启动资助项目（黔农科院人才启动项目[2009]006号）；贵州省科技计划资助项目（黔科合院所创能[2010]4002）
收稿日期：2011-12-28

更新日期/Last Update: 2014-09-03