[1]田凡,廖小锋,王莲辉,等.硬叶兜兰菌根真菌的分类鉴定[J].北方园艺,2017,41(24):116-122.[doi:10.11937/bfyy.20171417]
　TIAN Fan,LIAO Xiaofeng,WANG Lianhui,et al.Classification and Identifition of Mycorrhizal Fungi of Paphiopedilum micranthum[J].Northern Horticulture,2017,41(24):116-122.[doi:10.11937/bfyy.20171417]
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硬叶兜兰菌根真菌的分类鉴定

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第41卷 期数: 2017年24 页码: 116-122 栏目: 出版日期: 2017-12-25
Title:
Classification and Identifition of Mycorrhizal Fungi of
Paphiopedilum micranthum
作者:
田凡1; 廖小锋2; 王莲辉1; 朱国胜3; 桂阳3; 白新祥4
1.贵州省林业科学研究院，贵州 贵阳 550005；2.贵州科学院 山地资源研究所，贵州 贵阳 550001； 3.贵州农业科学院 现代中药材研究所，贵州 贵阳 550006；4.贵州大学 林学院，贵州 贵阳 550025
Author(s):
TIAN Fan1; LIAO Xiaofeng2; WANG Lianhui1; ZHU Guosheng3; GUI Yang3; BAI Xinxiang4
1.Guizhou Forestry Academy,Guiyang,Guizhou 550005;2.Institute of Mountain Resources,Guizhou Academy of Sciences,Guiyang，Guizhou 550001;3.Institute of Morden Chinese Medical Materials,Guizhou Academy of Agricaltural Sciences,Guiyang,Guizhou 550006;4.College of Forestry,Guizhou University，Guiyang,Guizhou 550025
关键词:
硬叶兜兰; 菌根真菌; 丝核菌; 序列分析; 鉴定
Keywords:
Paphiopedilum micranthum; mycorrhizal fungi; Rhizoctonia; rDNA ITS analysis; identifition
DOI:
10.11937/bfyy.20171417
文献标志码:
A
摘要:
以硬叶兜兰菌根真菌为研究对象，采用传统形态学结合rDNA-ITS区的PCR扩增、测序，采用NCBI BLAST在线软件对ITS区序列进行了分析，对硬叶兜兰菌根真菌进行分类鉴定。结果表明：对29株菌根真菌进行rDNA-ITS序列分析共鉴定出23个属，为瘤菌根菌属、胶膜菌属、丝核菌属，镰刀菌属、栓菌属、附毛菌属、拟迷孔菌属、小脆柄菇属、拟层孔菌属、毛壳属、侧耳属、轮枝菌属、弯孢聚壳属等，分离菌株最多的是瘤菌根菌属和胶膜菌属，分别占分离总数的16%和19%；鉴定到1个科，为胶膜菌科；12株优势菌根具有不同的宏观和微观形态，其均未有分生孢子，菌丝分枝点附近形成一个隔及细胞双核，11株菌株具念珠状细胞，大多数具有香味。
Abstract:
Classification and identification of mycorrhizal were associated with Paphiopedilum micranthum,the method of morphological was combined with the ITS gene fragments were PCR amplified and sequenced,the rDNA ITS regions were analyzed by means of the software of NCBI BLAST.The results showed that the 29 strains with different micro- and macro-morphological.Secondly,rDNA-ITS sequence analysis of 29 predo minant strains.The fungi were identified into 23 genus and 1 family,23 strains belong to Epulorhiza,Tulasnella,Rhizoctonia,Fusarium,Trametes,Trichaptum,Daedaleopsis,Psathyrella,Fomitopsi,Chaetomium,Pleurotus,Lecanicillium,Eutypella,Lecanicillium,Peniophorella,which their frequency were 16% and 19%.Only one family belonged to Tulasnellaceae.12 strains of dominant mycorrhiza had different microcosmic and macroscopic,none of them had conidiophore,near the hyphal branching-points formed of septum and conjugate nuclei,11 strains mycorrhiza with intestiniform cells,the most of mycorrhiza had a distinct aroma.

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备注/Memo

第一作者简介：田凡（1985-），女，硕士，工程师，现主要从事植物生物技术等研究工作。E-mail:tianfan850507@126.com.

责任作者：白新祥（1979-），男，博士，副教授，现主要从事观赏植物保育等研究工作。E-mail:xinxiangbai@126.com.
基金项目：贵州省农业攻关资助项目（黔科合支撑[2017]2524）；贵州省林业厅青年人才基金资助项目（黔林科合J[2013]2号）；贵州乡土珍稀花卉研发团队服务企业行动计划资助项目（黔科合平台人才[2016]5711）；贵州省社会发展资助项目（黔科合SY字[2015]3019号）。
收稿日期：2017-07-25

更新日期/Last Update: 2017-12-29