ZHANG Yi-zihan,ZHANG Qun,WANG Ping-ping,et al.Optimization of Culture Conditions on the Liparis Mycorrhizal Fungi[J].Northern Horticulture,2014,38(07):102-106.
羊耳蒜菌根真菌培养条件的优化
- Title:
- Optimization of Culture Conditions on the Liparis Mycorrhizal Fungi
- 文章编号:
- 1001-0009(2014)07-0102-05
- Keywords:
- Liparis; mycorrhizal fungi; culture conditions; growth rate
- 分类号:
- S 682.31
- 文献标志码:
- A
- 摘要:
- 以兰科羊耳蒜菌根为试材，通过分离、纯化、培养其菌根真菌，在单因素试验基础上利用正交实验方法筛选温度、碳源、氮源和培养基pH值等培养条件来建立羊耳蒜菌根真菌培养体系。结果表明：羊耳蒜菌根真菌菌落白色或浅黄色,呈均匀辐射状向外生长；生长面有稀松或较丰富的气生菌丝，带有透明液滴。通过对菌落生长速率的测定与分析，4种因素的最佳综合水平为碳源为葡萄糖，浓度为2%；氮源为氯化铵，浓度为0.5%；pH为7，温度为25℃；菌落的生长速率随着天数的递增而以较快的速度下降，在第10天基本趋于一个恒定的值。
- Abstract:
- Taking Liparis arethusa mycorrhizal fungi as material,through separation,purification and cultured,using orthogonal experimental method to set culture conditions that include temperature,carbon source,nitrogen source and medium pH value,the Liparis ?mycorrhizal fungi culture system which based on the single-factor test were established.The results showed that the white or pale yellow colonies of Liparis ?mycorrhizal fungi showed the uniform radial outward growth,growth surface of sloppy or rich aerial hyphae with transparent droplets.Through measurement and analysis of colony growth rate,four factors best level were set the carbon source was concentration of 2% glucose,the nitrogen source was concentration of 0.5% ammonium chloride,pH value of 7,temperature of 25℃.Colony growth rate decreased at faster speed,with the increase in the number of days,and finally tended to a constant value in the tenth.
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备注/Memo
第一作者简介：张艺子涵（1989-），女，硕士研究生，研究方向为农牧业生物工程。