[1]周智龙,蔺新兰,夏天,等.不同胡萝卜基因型SOC1-2启动子克隆及活性分析[J].北方园艺,2026,(12):11-18.[doi:10.11937/bfyy.20253715]
　ZHOU Zhilong,LIN Xinlan,XIA Tian,et al.Cloning and Activity Analysis of SOC1-2 Promoter in Different Carrot Genotypes[J].Northern Horticulture,2026,(12):11-18.[doi:10.11937/bfyy.20253715]
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不同胡萝卜基因型SOC1-2启动子克隆及活性分析

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 期数: 2026年12 页码: 11-18 栏目: 出版日期: 2026-06-30
Title:
Cloning and Activity Analysis of SOC1-2 Promoter in Different Carrot Genotypes
文章编号:
1001-0009(2026)12-0011-08
作者:
周智龙1; 蔺新兰1; 夏天1; 喻彬恒1; 李萌2; 林原1
（1.湖南农业大学 园艺学院，湖南省马铃薯工程技术研究中心，蔬菜生物学湖南省重点实验室，湖南 长沙 410128；2.中南林业科技大学 生命与环境科学学院，湖南 长沙 410004）
Author(s):
ZHOU Zhilong1; LIN Xinlan1; XIA Tian1; YU Binheng1; LI Meng2; LIN Yuan1
(1.College of Horticulture,Hunan Agricultural University/Hunan Province Potato Engineering Technology Research Center/Hunan Provincial Key Laboratory for Vegetable Biology,Changsha，Hunan 410128;2.College of Life and Environmental Sciences,Central South University of Forestry and Technology,Changsha,Hunan 410004)
关键词:
胡萝卜; SOC1; 启动子; 光照; 激素
Keywords:
Daucus carota L.; SOC1; promoter; light; hormones
分类号:
S631.2
DOI:
10.11937/bfyy.20253715
文献标志码:
A
摘要:
以6种不同的胡萝卜品种为试材，采用烟草瞬时表达系统和活体成像技术检测启动子活性的方法，研究了在光照、黑暗、不同浓度的激素赤霉素（GA3）和脱落酸(ABA)处理下的启动子活性，以期探究胡萝卜SOC1基因启动子的调控机制，解析胡萝卜开花分子机制。结果表明：光照和ABA均可诱导各启动子的活性，经36 h光照处理后，启动子活性显著上升，上升倍数可达6.52~12.06倍；经ABA处理后，启动子活性也均有显著上升，分别上升了1.66~5.60倍。而部分基因型启动子经GA3处理后被显著抑制，其活性分别下降了1.44~2.40倍。因此，光照和ABA可诱导DcSOC1-2启动子的表达，而部分基因型DcSOC1-2启动子会被GA3负向调控。
Abstract:
Taking six different carrot varieties as the test materials,the promoter activity was detected by tobacco transient expression system and in vivo imaging technology under the conditions of light,dark,and different concentrations of hormones GA3 and ABA,in order to explore the regulatory mechanism of the carrot SOC1 gene promoter and analyze the molecular mechanism of carrot flowering.The results showed that both light and ABA could induce the activity of each promoter.After 36 hours of light treatment,the promoter activity increased significantly,with an increase of about 6.52 to 12.06 times.After ABA treatment,the promoter activity also increased significantly,with an increase of about 1.66 to 5.60 times,respectively.However,some genotype promoters were significantly inhibited by GA3 treatment,and their activities decreased by about 1.44 and 2.40 times,respectively.Therefore,light and ABA could induce the expression of DcSOC1-2 promoter,while some genotype DcSOC1-2 promoters were negatively regulated by GA3.

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备注/Memo

第一作者简介：周智龙（2000-），男，硕士研究生，研究方向为农艺与种业。E-mail：2542872202@qq.com.责任作者：林原（1985-），女，博士，副教授，现主要从事马铃薯遗传育种、马铃薯抗逆、发育分子机制等研究工作。E-mail：linyuan@hunau.edu.cn.基金项目：湖南农业大学引进人才科研启动资助项目（540742000699）；长沙市自然科学基金资助项目（kq2402251）。收稿日期：2025-10-27

更新日期/Last Update: 2026-06-30