LI Yuqi,TANG Siyu,WANG Xiangru,et al.Transcriptome Analysis of Sea Buckthorn Source Composite Coating Agent Regulating the Expression of Browning Gene in Fresh Cut Apples[J].Northern Horticulture,2026,(12):19-29.[doi:10.11937/bfyy.20254341]
沙棘源复合涂膜剂调控鲜切苹果褐变基因表达的转录组学分析
- Title:
- Transcriptome Analysis of Sea Buckthorn Source Composite Coating Agent Regulating the Expression of Browning Gene in Fresh Cut Apples
- 文章编号:
- 1001-0009(2026)12-0019-11
- Keywords:
- genes; sea buckthorn polyphenol; sea buckthorn essential oil; fresh cut apples; browning; microcapsules
- 分类号:
- S793.6
- 文献标志码:
- A
- 摘要:
- 以沙棘多酚和沙棘精油为试材，基于微胶囊技术制备了沙棘源-大豆分离蛋白（SPI）复合涂膜剂并将其应用于鲜切苹果，采用转录组技术研究了苹果基因的表达情况，以期阐明复合涂膜剂对鲜切苹果褐变的调控机制,为今后鲜切苹果的品质维持提供参考依据。结果表明：转录组测序共获得6 377个差异表达基因（DEGs），并初步确定复合涂膜剂对褐变的调控与苯丙烷生物合成、类黄酮生物合成和亚油酸代谢等途径有关。进一步的分析表明，复合涂膜剂处理贮存4 d后，相比对照，多酚氧化酶（PPO）、过氧化物酶（POD）、黄酮醇合成酶（FLS）、磷脂酶 D（PLD）和分泌型磷脂酶A2（sPLA2）基因的相对表达量分别降低了23.06%、82.64%、56.62%、20.80%和43.73%，相关性分析表明,MdPPO、MdPOD、MdFLS、MdPLD和MdsPLA2等基因表达与鲜切苹果褐变均呈正相关，与复合涂膜剂抑制鲜切苹果褐变有关。
- Abstract:
- Taking sea buckthorn polyphenols and sea buckthorn essential oil as test materials,sea buckthorn-soybean protein isolate (SPI) composite coating agent was prepared based on microcapsule technology and applied to fresh cut apples.The expression of apple genes was studied by transcriptome technology,in order to elucidate the regulation mechanism of composite coating agent on browning of fresh cut apples,and provide reference for maintaining the quality of fresh-cut apples in the future.The results showed that a total of 6 377 differentially expressed genes (DEGs) were obtained by transcriptome sequencing.The pathways of phenylpropanoid biosynthesis,flavonoid biosynthesis and linoleic acid metabolism were preliminarily determined to be related to the regulation of composite coating agent on browning.Further analysis showed that the relative expression levels of polyphenol oxidase (PPO),peroxidase (POD),flavonol synthase (FLS),phospholipase D (PLD) and secretory phospholipase A2 (sPLA2) genes in the composite coating group decreased by 23.06%,82.64%,56.62%,20.80% and 43.73% respectively,compared with the control group after 4 days of storage. Correlation analysis showed that the expressions of MdPPO,MdPOD,MdFLS,MdPLD and MdsPLA2 were positively correlated with the browning of fresh cut apples,which was related to the inhibition of browning of fresh cut apples by the composite coatings.
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备注/Memo
第一作者简介：李雨奇（1995-），女，硕士，讲师，现主要从事果蔬贮藏与保鲜等研究工作。E-mail：liyuqi7868@163.com.基金项目：山西省基础研究计划自由探索类资助项目（202303021222258）；山西省高等学校科技创新资助项目（2023L373）；吕梁市重研发计划农业领域资助项目（2025NY26）。收稿日期：2025-12-18