WANG Mixue,SHENG Zhicun,CHEN Xiaolan.Optimization of Extraction and Characterization of Polysaccharide From Stropharia rugosoannulata Fermentation Mycelium[J].Northern Horticulture,2020,44(01):111-116.[doi:10.11937/bfyy.20191112]
大球盖菇发酵菌丝体多糖提取条件优化与结构分析
- Title:
- Optimization of Extraction and Characterization of Polysaccharide From Stropharia rugosoannulata Fermentation Mycelium
- Keywords:
- Stropharia rugosoannulata; mycelium polysaccharide; extraction process; structural characterization
- 文献标志码:
- A
- 摘要:
- 以大球盖菇发酵菌丝为试材，采用响应面法Box-Behnken设计研究了大球盖菇发酵菌丝体提取多糖最佳工艺条件，对其提取工艺进行优化，对结构进行初步表征。结果表明：最佳工艺条件为液料比26∶1 mL?g-1、提取温度82 ℃、提取时间3.5 h，大球盖菇菌丝体多糖提取量为15.12%。柱前衍生化高效液相色谱分析单糖组成，包含甘露糖、核糖、鼠李糖、葡萄糖、木糖、阿拉伯糖和果糖，对应摩尔比为0.76∶1.64∶0.65∶1.00∶1.24∶1.18∶0.20。红外光谱分析具有典型的糖特征吸收。该研究结果为大球盖菇液体培养菌丝体开发利用提供参考依据。
- Abstract:
- In order to study the optimum extraction conditions and preliminarily characterization of polysaccharide from fermentation mycelium of Stropharia rugosoannulata.Box-Behnken design of response surface method was employed to optimize the extraction process.The results showed that the liquid-solid ratio,extraction temperature,extraction time of optimum process conditions were 26∶1 mL?g-1,82 ℃,3.5 hours,respectively.Under these conditions,the polysaccharides yield was 15.12%.The monosaccharide composition analysis by pre-column derivatization HPLC method showed that the mycelium polysaccharide was consisted of mannose,ribose,rhamnose,glucose，xylose,arabinose and fructose in a molar ratio of 0.76∶1.64∶0.65∶1.00∶1.24∶1.18∶0.20.Meanwhile，infraredspectra showed the polysaccharide had a typical characteristic absorption of sugars.The results of this study would provide a theoretical basis for the development and utilization of mycelium from liquid fermentation of Stropharia rugosoannulata.
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备注/Memo
第一作者简介：王米雪（1990-），女，硕士，助教，研究方向为天然药物化学。E-mail：wmx13615192752@163.com.责任作者：陈晓兰（1979-），女，博士，副教授，研究方向为中药资源开发与应用。E-mail：cxl7972563@163.com.基金项目：国家自然科学基金青年科学基金资助项目（31702286）；江苏农牧科技职业学院科研资助项目（NSFPT201801）。收稿日期：2019-04-30