LI Zheng,GAO Qingyu,ZHANG Bingxiu,et al.Effect of Growth Regulator on Bud Differentiation of Callus of Vaccinium uliginosum L.[J].Northern Horticulture,2018,42(22):29-36.[doi:10.11937/bfyy.20181428]
生长调节剂对笃斯越橘愈伤组织芽分化的影响
- Title:
- Effect of Growth Regulator on Bud Differentiation of Callus of Vaccinium uliginosum L.
- Keywords:
- Vaccinium uliginosum L.; callus; growth regulator; differentiation bud
- 文献标志码:
- A
- 摘要:
- 以质地紧实、绿色且有活力的笃斯越橘优系SL1的叶片和无芽茎段的愈伤组织为试材，利用WPM改良培养基，采用单因子变量和多因素正交组合的试验方法，研究了3种细胞分裂素TDZ、ZT、6BA，2种生长素IBA、NAA和蔗糖在不同浓度不同组分下对笃斯越橘优系SL1的叶片和无芽茎段2种愈伤组织分化芽的影响，以期为笃斯越橘种质资源保存和优系筛选提供参考。结果表明：单独使用细胞分裂素诱导愈伤组织分化再生时，ZT较TDZ和6BA更加适宜愈伤组织诱导分化，且最佳浓度为2.0 mg·L-1，叶片和茎段愈伤组织分化率分别可达85.67%和89.00%；同时使用TDZ、ZT、IBA和NAA时，叶片愈伤组织诱导效果优于茎段愈伤组织，分化率最高可达100.00%和93.00%，其最佳组分均为TDZ 1.0 mg·L-1+ZT 3.0 mg·L-1+IBA 0.05 mg·L-1+NAA 0 mg·L-1；蔗糖浓度对于叶片和茎段愈伤组织芽分化也有影响，且最适蔗糖浓度分别为25 g·L-1和20 g·L-1。综上所述，叶片愈伤组织更加适合分化芽的形成，且最佳培养基为WPM培养基+蔗糖20 g·L-1+琼脂8 g·L-1+TDZ 1.0 mg·L-1+ZT 3.0 mg·L-1+IBA 0.05 mg·L-1。
- Abstract:
- Callus explants of the leaf and stem segments without buds of Vaccinium uliginosum L.SL1,which were compact,green and energetic,were used as the test materials in WPM medium.The effect of TDZ,ZT and 6BA,growth regulators of IBA,NAA and sucrose in different concentrations on bud differentiation were studied by using the singlefactor variable and multifactor orthogonal combination method in order to provide a reference for the preservation of germplasm resources and the selection of superior system in the future.The results showed that by using ZT alone to induce callus differentiation buds,ZT was more suitable for callus differentiation bud than TDZ and 6BA,and the best concentration was 2.0 mg·L-1,the callus differentiation rate of leaf and stem was up to 85.67% and 89.00%.When using TDZ,ZT,IBA and NAA,differentiation rate of leaf callus was better than that in the stem segment callus,and the rate of differentiation was up to 100.00% and 93.00%.The best components were TDZ 1.0 mg·L-1+ZT 3.0 mg·L-1+IBA 0.05 mg·L-1+NAA 0 mg·L-1.Sucrose concentration had effect on the bud differentiation of callus of leaf and stem segment,the best sucrose concentrations were 25 g·L-1 and 20 g·L-1.In conclusion,leaf callus was more suitable for differentiation bud formation,and the best medium was WPM+sucrose 20 g·L-1+agar 8 g·L-1+TDZ 1.0 mg·L-1+ZT 3.0 mg·L-1+IBA 0.05 mg·L-1.
相似文献/References:
[1]李松丽,黄欢欢,蔡小东.两种凝固剂对冰糖橙愈伤组织增殖及植株再生的影响[J].北方园艺,2013,37(22):108.
LI Song-li,HUANG Huan-huan,CAI Xiao-dong.Effects of Two Types of Gelling Agents on the Callus Proliferation and Plant Regeneration of Citrus sinensis L.Osbeck cv.Bingtang[J].Northern Horticulture,2013,37(22):108.
[2]闫海霞,黄昌艳,何荆洲,等.观赏凤梨组织培养的影响因素分析[J].北方园艺,2013,37(22):196.
YAN Hai-xia,HUANG Chang-yan,HE Jing-zhou,et al.Analysis of the Influence Factors in Tissue Culture of Ornamental Bromeliads[J].Northern Horticulture,2013,37(22):196.
[3]王 婷,智永祺,周 坚.长筒石蒜叶片薄层培养诱导愈伤组织发生的研究[J].北方园艺,2014,38(01):91.
WANG Ting,ZHI Yong-qi,ZHOU Jian.Study on Callus Induction of the Leaf Blades of Lycoris longitubaUsing Thin Cell Layer Culture[J].Northern Horticulture,2014,38(22):91.
[4]焦战战,张东向,赵静,等.黄芩愈伤组织中黄芩苷与过氧化物酶活性相关性分析[J].北方园艺,2013,37(13):108.
JIAO Zhan-zhan,ZHANG Dong-xiang,ZHAO Jing,et al.Analysis of the Correlation Between Baicalin and Peroxidase Activity in the Callus of Scutellaria baicalensi Georgi[J].Northern Horticulture,2013,37(22):108.
[5]罗君琴,徐建国,王平.不同培养条件对柑桔胚性愈伤组织离体诱导的影响[J].北方园艺,2013,37(13):125.
LUO Jun-qin,XU Jian-guo,WANG Ping.Effect of Different Culture Condition on Embryogenic Callus in Vitro of Citrus[J].Northern Horticulture,2013,37(22):125.
[6]马少梅.北海道黄杨再生体系建立研究进展[J].北方园艺,2013,37(13):208.
MA Shao-mei.Research Advances on the Establishment of Regeneration System of Euonymus japonicus[J].Northern Horticulture,2013,37(22):208.
[7]于 娜,董宽虎.白羊草成熟种子组织培养再生体系研究[J].北方园艺,2014,38(02):104.
YU Na,DONG Kuan-hu.Research on Tissue Culture of Regeneration System of the Mature Seeds of Bothriochloa ischaemum [J].Northern Horticulture,2014,38(22):104.
[8]张丽霞,王毅彰.植物生长调节剂对花籽山药愈伤组织诱导形成的影响[J].北方园艺,2013,37(14):115.
ZHANG Li-xiaWANG Yi-zhang.Effect of Plant Growth Substances on Callus Induction of Seed Yam Forming[J].Northern Horticulture,2013,37(22):115.
[9]李金英,赵春莉,张馥茜,等.黑穗醋栗愈伤组织诱导研究[J].北方园艺,2013,37(17):87.
LI Jin-ying,ZHAO Chun-li,ZHANG Fu-qian,et al.Study on the Callus Induction of Blackcurrant[J].Northern Horticulture,2013,37(22):87.
[10]于艳萍,韩善峰,赵登超,等.嫩枝扦插不同处理对“香玲”核桃组织再生能力的影响[J].北方园艺,2013,37(17):91.
YU Yan-ping,HAN Shan-feng,ZHAO Deng-chao,et al.Effect of Different Softwood Cutting Treatments on the Tissue Regenerating Ability of Juglans regia ’Xiangling’[J].Northern Horticulture,2013,37(22):91.
备注/Memo
第一作者简介：李征（1993），女，硕士研究生，研究方向为果树栽培育种及生物技术。Email:1076274503@qq.com.责任作者：高庆玉（1960），男，博士，教授，博士生导师，研究方向为果树育种栽培及育种。Email:gaoqingyu@tom.com.基金项目：公益性行业（农业）科研专项资助项目（201103037）。收稿日期：2018-07-03