LI Yajiao,GUO Jiufeng,LIU Xiaoting,et al.Influence of High Voltage Electrostatic on Physiological and Biochemical Indexes of Licorice During Seedling Stage[J].Northern Horticulture,2017,41(02):153-157.[doi:10.11937/bfyy.201702037]
高压静电场处理对甘草苗期生理生化指标的影响
- Title:
- Influence of High Voltage Electrostatic on Physiological and Biochemical Indexes of Licorice During Seedling Stage
- 文献标志码:
- A
- 摘要:
- 以甘草为试材,采用高压静电场处理甘草种子的方法，研究了电压及处理时间对甘草苗期几项生理生化指标的影响。结果表明：电场处理降低了苗期的相对含水量、提高了苗期的根冠比、根长和株高。随着甘草苗龄的增加，整体上叶绿素a含量18 kV组高于对照组，15 kV组低于对照组;整体上叶绿素b含量18 kV组在前45 d高于对照组，45~90 d低于对照组，而15 kV组一直低于对照组。电场处理组的可溶性蛋白含量在30~75 d低于对照组，75~90 d整体略高于对照组。
- Abstract:
- Taking licorice as test material by high voltage electrostatic field,physiological and biochemical indexes which influenced by different voltage and processing time during licorice seedling stage were studied.The results showed that the high voltage electrostatic field reduced relative water content and increased root shoot ratio and root length and plant height of seedling stage licorice;with the increase of licorice seedling age, the content of chlorophyll a increased first and then decreased.The 18 kV group was higher than the control group while the 15 kV group was inverse.And the content of chlorophyll b in the 18 kV group was higher than control group at the first 45 days then lower than the control group during 45-90 days,while the 15 kV group was always lower than the control group;the content of soluble protein in high voltage electrostatic field treated group was lower than control group during 30-75 days but higher than control group during 75-90 days.
参考文献/References:
[1]安文芝,蔺海明.甘草标准化生产技术[M].北京:金盾出版社,2008. [2]WEI S L,WANG W Q,WANG H.Study on licorice resources and their sustainable utilization in centfer and western area of China[J].中国中药杂志,2003,28(3):202-206. [3]WANG J Y,LIU C S,WANG W Q.The investigation of the licorice resources in northeast China[J].中国中药杂志,2003,28(4):308-312. [4]刘育辰,陈有根,王丹,等.甘草化学成分研究[J].药物分析杂志,2011,31(7):1251-1255. [5]王青,苗文娟,向诚,等.乌拉尔甘草化学成分研究[J].中草药,2012,43(10):1886-1890. [6]刘洋洋,刘春生,曾斌芳,等.甘草种质资源研究进展[J].中草药,2013,44(24):3593-3598. [7]李明.甘草的研究概况[J].甘肃中医学院学报,2009,17(3):59-63. [8]LIU H L，TAN Y,MONIKA N,et al.Arbuscular mycorrhizal fungal colonization of Glycyrrhiza glabra roots enhances plant biomass,phosphorus uptake and concentration of root secondary metabolites[J].J Arid Land,2014,6(2):186-194. [9]李昂,张鸣,蔺海明,等.西北风蚀区种植甘草对地表微环境和土壤物理性状的影响[J].干旱区资源与环境,2014,28(10):128-132. [10]李昂,张鸣,蔺海明,等.种植甘草预防土壤风蚀效应[J].草业科学,2014,31(5):839-843. [11]KATARZYNA D,PETR D,VALENTINA P,et al.Effect of pulsed electric field on the germination of barley seeds[J].LWT-Food Science and Technology,2012,47(1):161-166. [12]MURR L E.Mechanism of plant cell damage in an electrostatic field[J].Nature,1964,4926:1305-1306. [13]杨体强,朱海英,袁德政,等.电场对油葵种子萌发影响的有效时间[J].核农学报,2013,27(6):879-883. [14]方晶.高压电场对干旱胁迫下黄瓜种子萌发的影响[J].北方园艺,2014(13):41-43. [15]高雪红.高压静电场预处理黄豆对其幼芽生物成份的影响[D].西安:陕西师范大学,2008. [16]张刚.静电场对菜豆、青椒和茄子冷害的作用及生理生化影响[D].北京:中国农业大学,2005. [17]SIDAWAY.Influence of electric field on seed germination[J].Nature,1966,5045:303-305. [18]黄洪云.高压静电场对小麦种子生物学效应的物理机理的研究[J].种子，2015,34(6):99-101. [19]李美清,吴沿友,李青林.高压静电场对水培番茄生理指标与产量的影响[J].农业机械学报,2015,46(11):145-150. [20]赵瑞平,孙丰梅,白殿海,等.高压静电场处理对番茄果实硬度变化的影响[J].北方园艺,2015(15):32-36. [21]CHRISTIAN J E,SIMONE B,MICHAEL P,et al.Effects of nanosecond pulsed electric field exposure on Arabidopsis thaliana[J].IEEE Transactions on Dielectrics and Electrical Insulation,2009,16(5):1322-1328. [22]EVELINA C.The influence of an electric field on the growth of soy seedlings[J].Journal of Electrostatics,2008,66:417-420. [23]王清元,卢贵忠,赵玉清.高压静电场对水稻种子萌发的试验研究[J].云南业大学学报,2005,20(1):147-150. [24]陈花,吴俊林,李晓军,等.电场处理荞麦种子对其幼苗抗旱性的影响[J].陕西师范大学学报(自然科学版),2009,37(1):78-82. [25]李亚娇,郭九峰,王淑研,等.负高压静电场对甘草种子萌发的影响[J].种子，2015,34(11):19-22. [26]叶菊,蔺海明,邱黛玉,等.几种甘草(Glycyrrhiza)光合特性、形态特征及生物量比较[J].中国沙漠,2014,34(2):456-464. [27]刘遵春,冯爱英,扈惠灵.柿树叶片叶绿素含量测定方法比较研究[J].河南科技学院学报(自然科学版),2014,42(3):12-15. [28]刘彩云.白肋烟叶片低叶绿素含量的生理生化分析[J].烟草科技,2014(5):75-77. [29]张梦莹,赵玉娟,李倩竹,等.梅花鹿茸可溶性蛋白提取工艺及免疫活性[J].东北林业大学学报,2014,42(9):158-163. [30]赵艳,李燕燕.利用PTN系统快速解析转基因水稻种子可溶性蛋白非预期变异的来源[J].中国农业科学,2015,48(22):4397-4407. [31]李晓忱,依艳丽.磁场对根瘤菌可溶性蛋白和粗蛋白含量的影响[J].水土保持学报,2014,28(2):302-312. [32]姜沅彤,赵岩,蔡恩博,等.灵芝水溶性蛋白提取工艺优化[J].食品安全质量检测报告,2014,5(5):1340-1348.
相似文献/References:
[1]段国锋,王争争,李丽娟,等.高压静电场对菜豆生理活性的影响[J].北方园艺,2014,38(11):24.
DUAN Guo-feng,WANG Zheng-zheng,LI Li-juan,et al.Effect of High Voltage Electrostatic Field on Physiological Activity of Kidney Bean[J].Northern Horticulture,2014,38(02):24.
[2]常桂英,孙立梅,赵 雪,等.长白山刺果甘草与甘草化学成分的比较研究[J].北方园艺,2014,38(10):146.
CHANG Gui-ying,SUN Li-mei,ZHAO Xue,et al.Comparative Study on the Chemical Constituents of Changbai Mountain Glycyrrhiza pallidiflora and Glycyrrhiza uralensis [J].Northern Horticulture,2014,38(02):146.
[3]余蕾.微波法提取甘草中类黄酮的工艺研究[J].北方园艺,2014,38(07):130.
YU Lei.Research on Flavonoid Extraction From Glycyrrhiza uralensis Fisch by Microwave Method[J].Northern Horticulture,2014,38(02):130.
[4]刘美杰,郝彩琴.宁夏乌拉尔甘草经济施磷量研究[J].北方园艺,2013,37(01):155.
LIU Mei-jie,HAO Cai-qin.Study on Economic Phosphate Levels of Ningxia Ural Licorice[J].Northern Horticulture,2013,37(02):155.
[5]舒康云,陶永元,徐成东,等.一种可食性涂膜保鲜液对樱桃保鲜效果的影响[J].北方园艺,2013,37(23):137.
SHU Kang-yun,TAO Yong-yuan,XU Cheng-dong,et al.Fresh-keeping Effect of an Edible Film on Cherry Preservation[J].Northern Horticulture,2013,37(02):137.
[6]马尧.左家地区甘草氮肥施用量的研究[J].北方园艺,2012,36(21):146.
MA Yao.Study on the Nitrogenous Fertilizer Application Rate on Glycyrrhiza uralensis in Zuojia Area[J].Northern Horticulture,2012,36(02):146.
[7]郭艳茹,林洁.乙醇浸提甘草中总黄酮的工艺研究[J].北方园艺,2013,37(24):158.
GUO Yan-ru,LIN Jie.Research on the Extraction Technology of Total Flavonoids from Licorice[J].Northern Horticulture,2013,37(02):158.
[8]赵瑞平,孙丰梅,白殿海,等.高压静电场处理对番茄果实硬度变化的影响[J].北方园艺,2015,39(15):32.[doi:10.11937/bfyy.201515007]
ZHAO Ruiping,SUN Fengmei,BAI Dianhai,et al.Effect of High Voltage Electrostatic Field Treatment on Physiology Properties of Firmness in Stored Green Mature Tomatoes[J].Northern Horticulture,2015,39(02):32.[doi:10.11937/bfyy.201515007]
[9]李彩虹,王彩艳,王晓静.宁夏道地甘草重金属残留特征及污染风险评价[J].北方园艺,2016,40(18):159.[doi:10.11937/bfyy.201618039]
LI Caihong,WANG Caiyan,WANG Xiaojing.Heavy Metal Residual Characteristics of Liquorice and Pollutional Risk Assessment in Ningxia[J].Northern Horticulture,2016,40(02):159.[doi:10.11937/bfyy.201618039]
[10]杨 光,王 玉 莉,周 玉 兰,等.高压静电场处理菜豆种子效果观察[J].北方园艺,2006,30(04):0.[doi:10.11937/bfyy.200604014]
[J].Northern Horticulture,2006,30(02):0.[doi:10.11937/bfyy.200604014]
备注/Memo
第一作者简介：李亚娇（1990-），女，硕士研究生，研究方向为环境生物物理。E-mail:726450605@qq.com.责任作者：郭九峰（1964-），男，博士，教授，硕士生导师，现主要从事生物物理与生物技术等研究工作。E-mail:guojf@sina.com.基金项目：国家自然科学基金资助项目（51467014）。