SONG Shuang,LIU Yu,XU Feng,et al.Pathogenic Test of Four Different Pseudomonas tolaasii Strains to Pleurotus ostreatus CCEF-99[J].Northern Horticulture,2017,41(02):150-152.[doi:10.11937/bfyy.201702036]
四个托拉斯假单胞杆菌菌株对平菇CCEF-99的致病力测定
- Title:
- Pathogenic Test of Four Different Pseudomonas tolaasii Strains to Pleurotus ostreatus CCEF-99
- 文献标志码:
- A
- 摘要:
- 托拉斯假单胞杆菌(Pseudomonas tolaasii)是引起食用菌细菌性褐斑病的病原菌，该研究利用平皿对峙和子实体接种的方法，研究了4个不同托拉斯假单胞杆菌菌株对平菇CCEF-99菌丝体和子实体生长的影响，评价不同菌株的致病能力，为平菇品种抗病性鉴定提供备选鉴别菌株。结果表明：菌株JZB2120025的致病力最弱，病斑面积小，颜色呈淡黄色；菌株JCM21583的致病力最强，产生的病斑凹陷，颜色呈棕褐色，可作为平菇抗病性状检测的备选菌株。
- Abstract:
- Brown blotch disease，a serious mushroom disease，is usually caused by the bacterial pathogens of Pseudomonas tolaasii.In this study，the influence of four different P.tolaasii strains on the mycelium and fruit body growth of Pleurotus ostreatus CCEF-99 were tested to screen strains with strong pathogenicity and to provide backup strains for identifying resistance of mushroom varieties.The results showed that，the strain JCM21583 had the strongest pathogenic effect and could be used as identification strain to test the resistance ability of P.ostreatus against brown blotch disease.
参考文献/References:
[1]张瑞颖,胡丹丹,左雪梅,等.平菇和双孢蘑菇细菌性褐斑病研究进展[J].植物保护学报,2007,34(5):549-554. [2]HUTCHISON M L,JOHNSTONE K.Evidence for the involvement of the surface active properties of the extracellular toxin tolaasin in the manifestation of brown blotch disease symptoms by Pseudomonas tolaasii on Agaricus bisporus[J].Physiological and Molecular Plant Pathology,1993,42(5):373-384. [3]TOLAAS A G.A bacterial disease of cultivated mushrooms[J].Phytopathology,1915（5):51-54. [4]赵鸣.山东省主要棉区棉花黄萎病菌致病力分化及其生物学特性的研究[D].泰安:山东农业大学，2010. [5]徐晖,梁瑞玲.平菇CCEF99菌株特性与栽培要点[J].食用菌,2000(4):16-17. [6]徐岩岩,陈璐,李金萍,等.平菇细菌性褐斑病病原菌RT-PCR检测方法的建立及其应用[J].园艺学报,2013,40(1):169-178. [7]刘川,陈强,张金霞,等.平菇细菌性黄斑病发病因素分析[J].食用菌学报,2013,20(1):101-105. [8]赵肖静,石延霞,谢学文,等.平菇细菌性褐斑病诱抗菌株筛选及诱抗效果[J].食用菌学报,2013,20(1):96-100.
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XU Yanyan,LIU Xiaoguang,PENG Yanfang,et al.Effect of Refined Bitter Almond Shell Pyroligneous Acid on Brown Blotch Disease of Pleurotus ostreatus[J].Northern Horticulture,2016,40(02):148.[doi:10.11937/bfyy.201611039]
备注/Memo
第一作者简介：宋爽（1987-），女，硕士，研究实习员，现主要从事食用菌病虫害防控等研究工作。E-mail：songshuang1025@163.com.责任作者：刘宇（1968-），男，本科，研究员，现主要从事食用菌育种及栽培等研究工作。E-mail：ly6828@sina.com.基金项目：国家食用菌产业技术体系资助项目(CARS-24)；北京市科技计划重大资助项目(D151100004315002)；国家支撑计划资助项目(2013BAD16B03)。