HE Yanhong,WU Gaoyin,BAI Yue,et al.Callus Induction and Plant Regeneration of Armeniaca sibirica[J].Northern Horticulture,2016,40(04):101-106.[doi:10.11937/bfyy.201604027]
山杏愈伤组织诱导及植株再生
- Title:
- Callus Induction and Plant Regeneration of Armeniaca sibirica
- Keywords:
- Armeniaca sibirica; callus; plant regeneration
- 文献标志码:
- A
- 摘要:
- 以山杏成熟胚和胚乳为外植体，以MS为基本培养基进行组织培养，研究了基于山杏经愈伤组织途径建立的再生植株技术。结果表明：以胚乳为外植体诱导愈伤组织的适宜培养基为MS+6-苄基腺嘌呤（6-BA） 1.0 mg/L+2，4-二氯苯氧乙酸（2,4-D） 0.5 mg/L+蔗糖30 g/L+琼脂3 g/L；愈伤组织的继代及分化培养基为MS+6-BA 1.0 mg/L+萘乙酸（NAA）0.1 mg/L+蔗糖30 g/L+琼脂3 g/L；以成熟胚诱导愈伤组织和丛生芽增殖分化培养基为MS+6-BA 2.0 mg/L+蔗糖30 g/L+琼脂3 g/L；生根培养基为1/2MS+NAA 0.5 mg/L+蔗糖30 g/L+琼脂3 g/L。
- Abstract:
- Taking mature embryo and endosperm of Armeniaca sibirica as explants，using MS media with different combinations of hormones，the regeneration plant of Armeniaca sibirica was established.The results showed that the formula of MS+6-BA 1.0 mg/L+2,4-D 0.5 mg/L+sucrose 30 g/L+agar 3 g/L was better at the stage of inducing callus when endosperm was adopted as explant，and better medium of callus subculture and differentiation was MS+6-BA 1.0 mg/L+NAA 0.1 mg/L+sucrose 30 g/L+agar 3 g/L.MS+6-BA 2.0 mg/L+sucrose 30 g/L+agar 3 g/L was the optimal medium for callus induction from mature embryo and the multiple shoots from callus，and during the proliferating period of clustered shoots，the rooting medium was 1/2 MS+NAA 0.5 mg/L+sucrose 30 g/L+agar 3 g/L.
参考文献/References:
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备注/Memo
第一作者简介：何炎红（1979-），女，内蒙古呼和浩特人，博士，副教授，研究方向为森林培育。E-mail：hyh20012008@imau.edu.cn.责任作者：白玉娥（1968-），女，教授，研究方向为林木遗传育种。E-mail:bye666@163.com.基金项目：国家科技支撑资助项目（2013BAD14B02）。