QIAN Xueting,CHEN Wenqiang,DENG Baiwan,et al.Optimization of Liquid Culture Mediums of Auricularia auricular Strain Collected From Qinba Mountains by Response Surface Methodology[J].Northern Horticulture,2015,39(18):157-162.[doi:10.11937/bfyy.201518042]
秦巴山区黑木耳液体种培养基的响应面优化
- Title:
- Optimization of Liquid Culture Mediums of Auricularia auricular Strain Collected From Qinba Mountains by Response Surface Methodology
- 关键词:
- 黑木耳; 菌丝生物量; 液体种培养基; Box-Behnken设计
- 文献标志码:
- A
- 摘要:
- 以秦巴山区黑木耳菌株‘神农A8’为试材，在单因素试验的基础上，通过Box-Behnken设计对黑木耳液体种培养基进行响应面优化。结果表明：黑木耳液体种培养基最佳碳源、氮源分别是葡萄糖、牛肉粉，葡萄糖浓度、牛肉粉浓度以及KH2PO4浓度对菌丝生物量影响较大，优化得到黑木耳液体种培养基的配方是2.16%葡萄糖、0.48%牛肉粉、15%马铃薯、0.33% KH2PO4、0.1% MgSO4?7H2O，菌丝生物量的预测值为11.11 g/L，采用上述优化的黑木耳液体种培养基进行验证性试验，测得菌丝生物量为11.37 g/L，与预测值的相对误差为2.34%。因此，说明响应面法优化黑木耳液体种培养基是有效可行的。
- Abstract:
- Taking Auricularia auricular ‘Shennong A8’ collected from Qinba Mountains as materials，on the base of single facter experiments，the liquid culture medium of Auricularia auricular strain was optimized by Box-Behnken design.The results showed that the best carbon source was glucose and the best nitrogen source was beef powder，and the concentration of glucose，beef powder and KH2PO4 were the influential parameters on the mycelial biomass.And the best liquid culture medium was achieved and listed as follows：2.16% glucose，0.48% beef powder，15% potato，0.33% KH2PO4，0.1% MgSO4?7H2O.Under the optimal conditions，the mycelial biomass of Auricularia auricular was 11.37 g/L，compared with the theoretical value 11.11 g/L，the relative error was 2.34%.So the response surface method is effective and feasible for the optimization of liquid culture mediums of Auricularia auricular strain.
参考文献/References:
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备注/Memo
第一作者简介：钱雪婷（1990-），女，陕西汉中人，硕士研究生，现主要从事微生物资源利用开发等研究工作。E-mail:qianxueting163@163.com.责任作者：陈文强（1956-），男，陕西汉中人，教授，现主要从事微生物资源的保护与利用等研究工作。E-mail:wenqiangc@126.com.基金项目：陕西省科学技术研究发展计划资助项目（2014K01-16-03）；陕西省“13115”科技创新工程计划资助项目(2008ZDGC-04)。