CHENG Yumei,ZHAO Dan,KANG Yebin.Molecular and Morphological Identification of Peony Root Rot Pathogen[J].Northern Horticulture,2015,39(16):116-119.[doi:10.11937/bfyy.201516027]
牡丹根腐病病原的形态与分子鉴定
- Title:
- Molecular and Morphological Identification of Peony Root Rot Pathogen
- 文献标志码:
- A
- 摘要:
- 以河南洛阳各大主要牡丹园栽培的牡丹典型根腐病病株为试材,采用组织分离法获得纯菌株,并对所得菌株进行致病性测定、形态学鉴定及rDNA-ITS 序列分析,研究牡丹根腐病的病原菌种类。结果表明:分离获得的4个菌株在PDA培养基上均产生茂密、呈疏松棉絮状的菌丝及黄、红、紫等色素,大型分生孢子镰刀形或椭圆形,无色,多胞;小型分生孢子卵圆形至椭圆形,无色,单胞或双胞。致病性测定表明,4个菌株能侵染离体牡丹根尖使其变黑。在GenBank序列数据库中,F1~F4菌株的DNA序列与编号为HM214456.1和AB498917.1等的F.solaniITS区段DNA序列的同源性为99%~100%。据此,将河南洛阳牡丹根腐病的病原菌鉴定为F.solani。
- Abstract:
- Taking the typical infected plants of peony root rot as test materials,which from in mainly peony garden in Luoyang,Henan Province.The pathogens were isolated by tissue separation,tested according to the standard Koch’s postulation methods,and identified by the methods of microscopy and molecules,the pathogen species of peony root were studied.The results showed that four strains all produced thick,loose cotton wool hypha and yellow,red,violet colors in PDA.Macroconidium were falciform or ellipse,colourless,multi-cell;microconidia were oval to ellipse,colourless,one-celled or bicellular.those strains of Fusarium could infect in vitro peony root and make it black.The DNA sequence homology of F1 to F4 strains and Nos in GenBank sequence database of HM214456.1 and AB498917.1 F.solani was 99%-100%.Accordingly,the pathogen of peony root rot disease was identified as F.solani.
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备注/Memo
第一作者简介:成玉梅(1964-),女,本科,高级实验师,研究方向为农产品分析测试。E-mail:chengym999@163.com. 责任作者:康业斌(1964-),男,博士,教授,研究方向为植物免疫学。E-mail:kangyb999@163.com。基金项目:河南省科技厅重点科技攻关资助项目(102102110090)。