YANG Hong,YU He-ming,LI Xiao-yan,et al.Study on the Method of Measuring Pollen Viability and Pretreatment of Pollen[J].Northern Horticulture,2015,39(08):36-39.[doi:10.11937/bfyy.201508009]
猕猴桃花粉生活力测定方法及花药处理方法研究
- Title:
- Study on the Method of Measuring Pollen Viability and Pretreatment of Pollen
- 文章编号:
- 1001-0009(2015)08-0036-04
- 分类号:
- S 663.4
- 文献标志码:
- A
- 摘要:
- 花粉生活力测定与花药前期处理对人工授粉有着至关重要的意义。现以中华猕猴桃为试材，研究猕猴桃花粉生活力最佳的测定方法以及不同处理对花粉活力的影响。结果表明：TTC染色法测定猕猴桃幼蕾期花粉生活力低，而离体萌发法测定的花粉萌发率高，建议在生产中最好采用染色法与离体萌发法综合测定花粉生活力；花粉萌发率与花药脱离花瓣时间有关，花蕾期花朵采摘后应立即分离雄蕊与花瓣，以提高花粉萌发率；花粉干燥与环境条件有关，如环境已适宜干燥花粉，建议不要采用其它辅助干燥花粉的方法。
- Abstract:
- Method of measuring pollen viability and pretreatment of pollen have very important significance for artificial pollination.Taking Actinidia chinensis as materials，the best way of measuring pollen viability and the effect of different treatments were studied.The results showed that,for pollen of flower bud，the method of TTC，the pollen viability was low，and the method of germination in vitro and the pollen viability was high.So，the best way of measuring pollen viability was methods of staining and germination.The rate of pollen germination was associated with the time，and for flower bud，the best way was separation of stamens and petals immediately for improving the rate of pollen germination；Pollen drying was associated with environmental condition，if the environment was suitable for drying pollen，it didn’t need to use other drying methods.
参考文献/References:
相似文献/References:
[1]朴 锦,王 坤,范惠明,等.关苍术的开花动态和花蕊形态及花粉活力研究[J].北方园艺,2014,38(12):142.
PIAO Jin,WANG Kun,FAN Hui-ming,et al.Reseach on Flowering Dynamic,Flower Morphology and Pollen Viability of Atractylodes japonica [J].Northern Horticulture,2014,38(08):142.
[2]张 浩,周会玲,张晓晓,等.“金香”猕猴桃果实冷藏最适温度研究[J].北方园艺,2014,38(13):126.
ZHANG Hao,ZHOU Hui-ling,ZHANG Xiao-xiao,et al.The Researches of Optimum Temperature of ‘Jinxiang’ Kiwifruit During Cold Storage[J].Northern Horticulture,2014,38(08):126.
[3]金方伦,敖学熙,张发维,等.中华猕猴桃结果蔓上果实节位与生长发育动态分析[J].北方园艺,2014,38(13):15.
JIN Fang-lun,AO Xue-xi,ZHANG Fa-wei,et al.Analysis on the Growth and Development Dynamic of the Kiwi Fruit[J].Northern Horticulture,2014,38(08):15.
[4]赵俊侠,田小曼.新西兰猕猴桃管理经验对我国果业发展的启示[J].北方园艺,2014,38(11):167.
ZHAO Jun-xia,TIAN Xiao-man.The Inspiration From New Zealand Kiwi Fruit Management Experience on the Chinese Fruit Industry[J].Northern Horticulture,2014,38(08):167.
[5]金方伦,冯世华,张发维,等.不同留果量对中华猕猴桃后熟期果实品质的影响[J].北方园艺,2014,38(10):26.
JIN Fang-lun,FENG Shi-hua,ZHANG Fa-wei,et al.Effect of Different Fruit Retaining on Fruit Quality of Kiwi at Maturation Period[J].Northern Horticulture,2014,38(08):26.
[6]涂美艳,庄启国,马凤仙,等.猕猴桃溃疡病秋冬季综合防控技术[J].北方园艺,2014,38(04):112.
[7]陈永安,刘艳飞,陈 鑫,等.不同施肥措施对猕猴桃叶片营养状况及果实品质与产量的影响[J].北方园艺,2014,38(01):169.
CHEN Yong-an,LIU Yan-fei,CHEN Xin,et al.Effect of Different Fertilizing Ways on the Leaves Nutrition Status,Fruit Quality and Yield of Kiwifruit[J].Northern Horticulture,2014,38(08):169.
[8]严婷,郭军战,徐龙光.文冠果优良单株开花生物学特性研究[J].北方园艺,2014,38(05):58.
YAN Ting,GUO Jun-zhan,XU Long-guang.Research on Flowering Biological Characteristics of Superior Individual Plant of Xanthoceras sorbifoliaBunge[J].Northern Horticulture,2014,38(08):58.
[9]于安民,杨锦芬,詹若挺,等.贮存条件对阳春砂花粉活力的影响[J].北方园艺,2013,37(10):147.
YU An-min,YANG Jin-fen,ZHAN Ruo-ting,et al.Effect of Different Preservation Conditions on Pollen Viability of Amomum villosum Lour.[J].Northern Horticulture,2013,37(08):147.
[10]曲雪艳,魏英,刘科鹏,等.奉新猕猴桃叶片营养及果实品质的测定与分析[J].北方园艺,2014,38(07):24.
QU Xue-yan,WEI Ying,LIU Ke-peng,et al.Measurement and Analysis on Leaves Nutrients and Fruit Quality From Kiwifruit in Fengxin County[J].Northern Horticulture,2014,38(08):24.
备注/Memo
第一作者简介：杨红（1967-），女，硕士，教授，现主要从事生物学等研究工作。E-mail：657073699@qq.com.