ZHANG Jian-ying,XU Chuan-ling,SHEN Hai-long,et al.Comparative Analysis of Induction and Somatic Embryo genesis of Different Origin in Sorbus pohuashanesis Hedl.[J].Northern Horticulture,2014,38(13):102-105.
不同产地花楸树愈伤组织诱导和体胚发生比较分析
- Title:
- Comparative Analysis of Induction and Somatic Embryo genesis of Different Origin in Sorbus pohuashanesis Hedl.
- 文章编号:
- 1001-0009（2014）13-0102-04
- Keywords:
- Sorbus pohuashanesis; induction of callus; somatic embryogenesis; origin; plant growth regulators
- 分类号:
- S 718.43
- 文献标志码:
- A
- 摘要:
- 以花楸树离体成熟和未成熟合子胚为外植体，研究了不同产地、不同发育时间及不同植物生长调节剂组合对花楸树愈伤组织诱导和体胚发生的影响。结果表明：以成熟合子胚为外植体，MS+2.0 mg/L NAA+1.5 mg/L 6-BA对诱导愈伤组织和体胚发生效果好；不同产地合子胚的体胚诱导率排序为敦化>哈尔滨>五营>山河。以未成熟合子胚为外植体，MS+0.05 mg/L 2,4-D+1.0 mg/L 6-BA愈伤诱导率高，体胚发生在MS+0.2 mg/L 2,4-D+1.0 mg/L 6-BA最适宜；不同产地合子胚的体胚诱导率排序为延吉>哈尔滨>伊春。
- Abstract:
- Taking the mature and immature zygotic embryos of Sorbus pohuashanensis Hedl.as explants, the effect of areas,development time and combinations of plant growth regulators on the induction of somatic embryos and callus induction were studied.The results showed that mature zygotic embryos were used as explants，the effect of MS+2.0 mg/L NAA+1.5 mg/L 6-BA on callus induction and somatic embryogenesis was good.Different origin of somatic embryogenesis differences for Dunhua>Harbin>Wuying>Shanhe.In order to immature zygotic embryos as explants，MS+0.05 mg/L 2,4-D+1.0 mg/L 6-BA callus induction rate was high，somatic embryogenesis in MS+2,4-D 0.2 mg/L+1.0 mg/L 6-BA was the most suitable.Different origin of somatic embryogenesis differences for Yanji>Harbin>Yichun.
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备注/Memo
第一作者简介：张建瑛（1980-），女，吉林松原人，助理研究员，现主要从事林木种苗培育工作。E-mail：zhangjianying1124@126.com.