LU Yanjun.Study on Factors Affecting Callus Initiation From Bruguiera gymnorrhiza (L.) Savigny[J].Northern Horticulture,2016,40(15):92-96.[doi:10.11937/bfyy.201615023]
木榄愈伤组织诱导影响因子研究
- Title:
- Study on Factors Affecting Callus Initiation From Bruguiera gymnorrhiza (L.) Savigny
- Keywords:
- Bruguiera gymnorrhiza; callus induction; explant browning; cytokinins
- 文献标志码:
- A
- 摘要:
- 以木榄幼叶为外植体，进行其愈伤组织诱导的影响因子研究，通过灭菌条件、培养基成分、添加剂种类、外源植物激素浓度试验，比较外植体褐化率与愈伤组织诱导率。结果表明：75%乙醇灭菌30 s后在2%（体积分数v/v）NaClO溶液灭菌20 min是木榄幼叶表面消毒适宜条件；氨基酸培养基是基础培养基；45 ℃热激与添加1.0 mmol?L-1抗坏血酸均可降低褐化率；AA培养基＋0.50 mg?L-1 2,4-D＋0.50 mg?L-1 TDZ＋1.00 mmol?L-1维生素C或AA培养基＋0.50 mg?L-1 2,4-D＋0.50 mg?L-1 CPPU＋1.00 mmol?L-1维生素C均可得大于50%的愈伤诱导率。因此，克服外植体褐化与应用CPPU或TDZ作为细胞分裂素是木榄愈伤组织诱导成功的关键。
- Abstract:
- The factors affecting callus initiation from magrove plant Bruguiera gymnorrhiza was investigated with the young leaves as explant.Sterilization conditions，medium components，kinds of additive，different concentration of plant growth regulator combination were tested and callus induction rate was calculated.The results showed that 75% alcohol 30 seconds with 2% (volumn fraction w/v) NaClO 20 minutes was the best condition of surface sterilization；amino acid (AA) medium was basic medium；browning rate was inhibited by heat-shock at 45 ℃ and ascorbic acid；AA medium+0.50 mg?L-1 2,4-D+0.50 mg?L-1 TDZ+1.00 mmol?L-1 vitamin C or AA medium+0.50 mg?L-1 2,4-D+0.50 mg?L-1 CPPU combination were optimal callus induction medium，in which over 50% of callus induction rate was obtained.Therefore，explant browning prevention and application of CPPU or TDZ as cytokinine were the key to success in callus induction from Bruguiera gymnorrhiza.
参考文献/References:
[1]AL-BAHRANY A M,AL-KHAYRI J M.Micropropagation of grey mangrove Avicennia marina[J].Plant Cell Tissue and Organ Culture,2003,72(1):87-93. [2] MOHAMED A D.Mangrove forests：Valuble resources under the threat of development[J].Ocean Yearbook,1996（12）:247-269. [3]OGITA S,YEUNG E C,SASAMOTO H,et al.Histological analysis in shoot organogenesis from hypocotyl explants of Kandelia candel(Rhizophoraceae)[J].Journal of Plant Research,2005,117(6):457-464. [4]MOORTHY P,KATHIRESAN K.Photosynthetic pigments in tropical mangroves:impacts of seasonal flux of uv-b radiation and other environmental attributes[J].Botany Marina,1997,40(4):341-350. [5]SRINIVASA R,EGANATHAN P,ANAND A,et al.Protocol for in vitro propagation of Excoecaria agallocha L.，a medicinally important mangrove species[J].Plant Cell Reports,1998,17(11):861-865. [6]EGANATHEN P,RAO C.Manual on vegetative and micropropagation of mangroves[M].M S Swaminathan Research Foundation,Chennai，India,2001. [7]AKATSU M,HOSOI Y,SASAMOTO H,et al.Purine metabolism in cells of a mangrove plant,Sonneratia alba，in tissue culture[J].Journal of Plant Physiology,1996,149(1-2):133-137. [8]KAWANA Y,SASAMOTO H.Stimulation effects of salts on growth in suspension culture of a mangrove plant，Sonneratia alba，compared with another mangrove,Bruguiera sexangula and non-mangrove tobacco BY-2 cells[J].Plant Biotech,2008,25(2):151-155. [9]MIMURA T,MIMURA M,WASHITANI-NEMOTO S,et al.NaCl-dependent growth，ion content and regeneration of calluses initiated from the mangrove plant，Bruguiera sexangula[J].Journal of Plant Research,1997a,110(1):31-36. [10]MIMURA T,MIMURA M,WASHITANI-NEMOTO S,et al.Efficient callus initiation from leaf of mangrove plant，Bruguiera sexangula in amino acid medium:effect of nacl on callus initiation[J].Journal of Plant Research,1997b,110(1):25-29. [11]KAAI F K Y,SASAMOTO H.The relation between recalcitrancy of a mangrove plant，Kandelia obovata，and high endogenous level of abscisic acid[J].Plant Cell Tissue and Organ Culture,2008,94(2):125-130. [12]李莎.植物组织培养中外植体褐变的研究进展[J].陕西林业科技,2008(1):7-10. [13]姚洪军,罗晓芳.植物组织培养外植体褐变的研究进展[J].北京林业大学学报,2004,15(4):426-428. [14]林益明,向平,林鹏.红树林单宁的研究进展[J].海洋科学,2005,29(3):56-63. [15]MAIO A D,LIS H,GERSHONI J M,et al.Identification of glycoproteins that are receptors for peanut agglutinin on immature(cortical)mouse thymocytes[J].Febs Letters,1986,194(1):28-32. [16]MARTIN-DIANA A H,RICO D,BARRY-RYAN C,et al.Effect of heat shock on browning-related enzymes in minimally processed iceberg lettuce and crude extracts[J].Bioscience Biotechnology and Biochemistry,2005,69(9):1677-1685. [17]杨玲,黄绵佳,葛红.热激处理在抑制植物组织褐变中的应用[J].热带农业科学,2007,27(3):62-68. [18]LOAIZA-VELARDE J.Heat shocks applied either before or after wounding reduce browning of lettuce leaf tissue[J].Journal of the American Society for Horticulturalence,2001,126(2):227-234. [19]陈吉裕,张文玲.延龄草无菌培养体系的建立及防褐变措施[J].中国实验方剂学杂志,2016,22(5):40-44. [20]耿会玲,田鹏,麻妙锋.PUD类细胞分裂素的研究与应用[J].动物医学进展,2003,24(2):58-60. [21]PREECE J E,HUETTEMAN C A,ASHBY W C,et al.Micro-and cutting propagation of silver maple.I.results with adult and juvenile propagules[J].Journal of the American Society for Horticultural Science American Society for Horticultural Science,1991,116(1):142-148. [22]刘淼,曹后男,宗成文,等.TDZ对牛皮杜鹃叶片分化及继代增殖的影响[J].西北农业学报,2012,21(12):158-162. [23]LI Z J,TRAORE A,MAXIMOVA S,et al.Somatic embryogenesis and plant regeneration from floral explants of Cacao (Theobroma cacao L.) using thidiazuron[J].In vitro Cellular and Developmental Biology Plant,1998,34(4):293-299. [24]张月琴,陈耀锋,王晶晶,等.TDZ预处理及激素诱导对小麦茎尖丛生芽形成的影响[J].麦类作物学报,2014,34(7):990-996. [25]AHMED M R,ANIS M.Changes in activity of antioxidant enzymes and photosynthetic machinery during acclimatization of micropropagated Cassia alata L.plantlets[J].In vitro Cellular and Developmental Biology Plant,2014,139(1):1-9. [26]高金山,边庆华,张永忠,等.细胞分裂素CPPU的研究进展[J].农药,2006,45(3):151-154.
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备注/Memo
作者简介：鲁彦君（1984-），女，博士，讲师，现主要从事植物逆境生理教学与科研工作。E-mail：luyanjun032@126.com.基金项目：中央高校基本科研业务费专项资金资助项目（2452015044）；西北农林科技大学博士科研启动基金资助项目（2013BSJJ049）。