HUANG Jun-xuan,LIU Yan-jun,LI Jian-ke,et al.Regeneration of Cauliflower by in vitro Flower Bulb[J].Northern Horticulture,2014,38(05):88-90.
以花椰菜花球为外植体的离体再生体系的建立
- Title:
- Regeneration of Cauliflower by in vitro Flower Bulb
- 文章编号:
- 1001-0009（2014）05-0088-03
- Keywords:
- cauliflower; hormone; regeneration; transformation
- 分类号:
- S 635.3
- 文献标志码:
- A
- 摘要:
- 以花椰菜花球切片为外植体，在含有不同激素的培养基上进行再生芽的诱导、伸长和生根培养，从中筛选出最适的培养基配方。结果表明：最适的分化培养基为MS+1.0 mg/L BA+0.5 mg/L IAA；最适的伸长培养基为MS+0.5 mg/L IAA；最适的生根培养基为1/2MS+1.0 mg/L IAA。该试验结果为花椰菜基因转化及其进一步组培研究奠定了基础。
- Abstract:
- Taking cauliflower slices as explants to filter out the most suitable culture medium on medium containing different hormones to induce regeneration buds，elongation and rooting.The results showed that the optimal differentiation medium was MS+1.0 mg/L BA+0.5 mg/L IAA；optimal elongation medium was MS+0.5 mg/L IAA；optimum rooting medium was 1/2MS+1.0 mg/L IAA.The regeneration system may be used for gene transformation and tissue culture research of cauliflower further.
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备注/Memo
第一作者简介：黄俊轩（1973-），男，广东河源人，硕士，副教授，现主要从事园林植物等研究工作。E-mail:huangjunxuan@sina.com.