GAO Ye-hua,GUO Peng-wei,GAO Ri,et al.Study on Rooting and Acclimatization in the Process of Tissue Culture of Aronia melanocarpa [J].Northern Horticulture,2013,37(09):105-108.
黑果腺肋花楸组培苗生根培养及驯化的研究
- Title:
- Study on Rooting and Acclimatization in the Process of Tissue Culture of Aronia melanocarpa
- 文章编号:
- 1001-0009(2013)09-0105-04
- 关键词:
- 激素; NO3-∶NH4+比例; 活性炭; 栽培基质
- Keywords:
- hormone; the rate of NO3-∶NH4+; activated charcoal; growth media
- 分类号:
- S 685.99
- 文献标志码:
- A
- 摘要:
- 以黑果腺肋花楸组培苗为试材，研究了MS培养基浓度、IBA浓度、NO3-/NH4+及活性炭浓度比例对生根的影响，并在不同栽培基质上进行驯化筛选。结果表明：适宜黑果腺肋花楸组培苗生根的培养基为1/2MS（改良）+0.6 mg/L IBA+1.5 g/L活性炭+30 g/L蔗糖+7 g/L琼脂;培养基浓度为1/2MS时适宜其生根，根长达到3.2 cm，根数目达到3.1;生长素IBA浓度为0.6 mg/L时，植株生长健壮，根长为3.4 cm;NO3-∶NH4+为3∶3时，根数目达4.3条，生根率为100.0%。加入活性炭浓度为1.5 g/L时，植株直立健壮、须根多且为白绿色，叶片舒展。腐质土∶沙土=1∶1为适宜黑果腺肋花楸移植苗的基质。室外自然条件下驯化，移植苗已适应了延吉地区气候。
- Abstract:
- Taking the tissue-cultured plantlets of Aronia melanocarpa as material，the effects of MS media concentrations，IBA concentrations，activated charcoal concentrations，rate of NO3-∶NH4+ on rooting of Aronia melanocarpa were studied，and growth media on root growth was acclimatized.The results showed that the best media for rooting of Aronia melanocarpa was 1/2MS+0.6 mg/L IBA+1.5 g/L activated charcoal+30 g/L sucrose+7 g/L agal.1/2MS was the suitable rooting medium with root length 3.2 cm.The hormone treatment of IBA 0.6 mg/L was better for growth with the root length 3.1 cm.When the rate of NO3-∶NH4+ was 3∶3，root amount was 4.3 with rooting rate 100.0%.The activated charcoal concentration of 1.5 g/L was significantly higher than other treatments，and the plant was healthy and strong，with unfolding leaves and much white and green fibrous root.The rate of humus∶sandy soil 1∶1 was the suitable medium for Aronia melanocarpa and the transplants had adapted to the regional climate of Yanji basically.
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备注/Memo
第一作者简介：高晔华（1987-），女，在读硕士，研究方向为园艺植物生物技术及栽培生理。