JIANG Xiang-hui,YUAN Jing,ZHU Jun-wei,et al.Effect of Post-Harvest Processing and Extraction Methods on Total Saponin Content of Cyclocarya paliurus[J].Northern Horticulture,2014,38(23):120-123.
青钱柳采后加工及提取方法对青钱柳总皂苷含量的影响
- Title:
- Effect of Post-Harvest Processing and Extraction Methods on Total Saponin Content of Cyclocarya paliurus
- 文章编号:
- 1001-0009（2014）23-0120-04
- 分类号:
- S 792.159
- 文献标志码:
- A
- 摘要:
- 以青钱柳为试材，采用直接烘干、烫后烘干、边搓边烘、烫后边搓边烘4种方法，分别在不同温度下烘干后测定药材中总皂苷含量；以药材粉碎与否、料液比、提取时间、提取次数为考察因素，采用L9（34）正交实验方法进行不同提取方法对青钱柳总皂苷的含量的影响分析，研究采后加工及提取方法对青钱柳总皂苷含量的影响。结果表明：不同采后加工方法得到的青钱柳总皂苷含量为2.59%~6.52%，在100℃和80℃下边搓边烘处理的青钱柳药材总皂苷含量最高；料液比和粉碎程度是影响青钱柳总皂苷提取率的关键因素，当样品过40目筛后，样品与甲醇比为1∶20时提取率最低(仅为33.48%）。该研究认为，80℃边搓边烘的青钱柳总皂苷传统加工工艺可加速药材的干燥，且保留了较多的皂苷成分，高强度粉碎和合适的料液比能有效提高青钱柳总皂苷的提取率。
- Abstract:
- Taking Cyclocarya paliuru as material，4 post-harvest processing methods (direct dry，drying after scalding by boiling water，drying while twisting，and drying while twisting after scalding by boiling water) were used to determine the content of total saponin.An orthogonal test L9(34) with crushed degrees，solid-liquid ratio，extraction time and extraction times as factors were designed to analyze the extraction ratio of total saponin in Cyclocarya paliurus,and to reveal the content variation of total saponin in Cyclocarya paliurus by post-harvest processing and extraction methods.The results showed that the content of total saponin was ranged from 2.59% to 6.52% in different samples processed by different processing methods，the total saponin was the highest in Cyclocarya paliurus by drying while twisting at 80℃ and 100℃.Solid-liquid ratio and crushed degrees were the key factor in influencing factors of the extraction yield，after 40 mesh passing sieve and solid-liquid ratio of 1∶20，the total saponin was the lowest，the extraction ratio was only 33.48%.Drying while twisting at 80℃ was the best post-harvest processing method，which could help to dry the fresh herbs and improve the accumulation of total saponin，it could effectively increase extracting rate of total saponin content of Cyclocarya paliurus under high crushing and appropriate solid-liquid ratio.
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备注/Memo
第一作者简介：蒋向辉（1974-），男，博士，副教授，现主要从事药用植物成分等研究工作。E-mail：jxfei789@163.com.