ZHANG Bin,TIAN Li-ping,ZHANG Yi-hui,et al.Determination of Total Flavonoids and Total Saponins in Different Growth Phases and Different Parts of Citrullus lanatus ssp.vulgaris var.megalaspermus Lin et Chao[J].Northern Horticulture,2015,39(09):109-112.[doi:10.11937/bfyy.201509030]
籽瓜不同生育期不同部位总黄酮和总皂苷含量测定
- Title:
- Determination of Total Flavonoids and Total Saponins in Different Growth Phases and Different Parts of Citrullus lanatus ssp.vulgaris var.megalaspermus Lin et Chao
- 文章编号:
- 1001-0009（2015）09-0109-04
- Keywords:
- Citrullus lanatus; different growth phases; different parts; total flavonoids; total saponins; spectrophotometric method
- 分类号:
- S 642.9
- 文献标志码:
- A
- 摘要:
- 以籽瓜为试材，分别以芦丁和葫芦素B为标准品，采用亚硝酸钠-硝酸铝显色法和香草醛-硫酸比色法分别测定籽瓜中总黄酮和总皂苷含量，为籽瓜质量控制提供科学依据。结果表明：总黄酮线性方程为Y=0.0059C-0.0081，R2=0.9994；总皂苷线性方程为Y=0.005C-0.0335，R2=0.9991。数据表明这2种测定方法准确,且重复性好。籽瓜不同部位的总黄酮和总皂苷含量分别在这2种方法下随生长时期变化而变化，根据这些数据可以为籽瓜的综合开发利用提供参考依据。
- Abstract:
- Taking Citrullus lanatus as test material，the contents of total flavonoids and total saponins from Citrullus lanatus was determined by sodium nitrite-aluminum nitrate chromomeric method and vanillin-sulfuric acid method，which provide a scientific basis for the quality control of Citrullus lanatus.To determinate the contents of total flavonoids and total saponins from Citrullus lanatus，the rutin and cucurbitacin B were used as standard product.The results showed that，the linear equations of total flavonoids and total saponins determination were respectively Y=0.0059C-0.0081，R2= 0.9994 and A=0.005C-0.0335，R2=0.9991.Data showed that the contents of total flavonoids and total saponin in different parts of Citrullus lanatus with growth period changes.The method was accurate and reproducible，which provided a theoretical basis for the comprehensive development and utilization of Citrullus lanatus.
参考文献/References:
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备注/Memo
第一作者简介：张彬（1989-），女，硕士研究生，研究方向为新疆特色药用植物资源与GAP规范化种植技术。E-mail：584768851@qq.com.